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Detection reagent strip for interleukin-6 and application thereof

An interleukin and detection reagent technology, which is applied in the field of interleukin-6 detection reagent strips, can solve the problems of difficult routine maintenance of machines, low degree of automation, poor quantitative accuracy, etc., so as to improve detection safety and accuracy, Short detection time, the effect of avoiding contamination

Pending Publication Date: 2022-03-22
SUZHOU SYM BIO LIFESCI CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Among them, although the ELISA method has been used clinically for nearly two decades, it still has some fatal shortcomings, such as poor quantitative accuracy, long operation time, low degree of automation, and susceptibility to interference; the chemiluminescence method has high sensitivity and good specificity, but The general chemiluminescent machine has a complex liquid cleaning system, and the machine is generally relatively large. Even if it is made smaller by optimizing the design, it also makes the daily maintenance of the machine difficult.

Method used

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  • Detection reagent strip for interleukin-6 and application thereof

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Embodiment 1

[0028] This embodiment provides an interleukin-6 detection reagent strip, the detection reagent strip includes a number of holes arranged side by side; the holes are respectively provided with a magnetic rod cover for setting a magnetic rod, a magnetic rod coated with an antibody At most one of beads, labeling reagents, magnetic bead wash solution or pre-excitation solution.

[0029] The magnetic bar cover is matched with a special up-suction magnetic bead transfer gun. When performing interleukin-6 detection, the magnetic bar can go deep into the liquid to quickly extract the magnetic beads, and the magnetic bar cover is a disposable consumable, which can It prevents magnetic beads from contaminating the magnetic rod, and at the same time makes the elution of magnetic beads easier; each reagent is sealed and stored in the corresponding hole using heat-sealing film technology, which effectively prevents reagents from crossing holes and thus solves the transportation problem.

...

Embodiment 2

[0033] This embodiment provides a detection method using a detection reagent strip as described in Embodiment 1, the steps comprising:

[0034] S1. Add the sample to the No. 4 well, and at the same time transfer the coated antibody magnetic beads and the labeled reactant to the No. 4 well, and combine them into an immune complex through an immune reaction, and the immune complex is Magnetic microspheres coated with anti-interleukin-6 monoclonal antibody-interleukin-6 antigen-anti-interleukin-6 monoclonal antibody labeled with acridinium ester;

[0035] S2. Transfer the immune complexes to the No. 7-9 wells sequentially, so as to remove unbound antigens or the labeled reactants by washing;

[0036] S3. Transfer the immune complex to the No. 10 hole, add an excitation solution, and measure the luminescence value of the chemiluminescent reaction, and the luminescence value is positively correlated with the concentration of interleukin-6 in the sample.

Embodiment 3

[0038] This embodiment provides a detection kit containing the detection reagent strip as described in Embodiment 1, and also includes a calibrator.

[0039] Wherein, the calibrator is BSA solution containing interleukin-6 antigen.

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Abstract

The invention relates to a detection reagent strip for interleukin-6. The detection reagent strip comprises a plurality of hole sites which are arranged side by side, and at most one of a magnetic bar sleeve for sleeving a magnetic bar, a coated antibody magnetic bead, a labeling reactant, a magnetic bead cleaning solution or a pre-excitation solution is arranged in each hole site. According to the interleukin-6 detection reagent strip provided by the invention, a chemiluminescence technology is combined with immune magnetic particles, and a reaction system close to a homogeneous phase is provided; compared with the prior art, the detection sensitivity and specificity are higher, and better performance parameters are achieved.

Description

technical field [0001] The invention relates to the field of immune analysis, in particular to a reagent strip for detecting interleukin-6 and its application. Background technique [0002] Interleukins are a class of cytokines produced by and act on various cells. Initially, it refers to cytokines that are produced by leukocytes and play a regulatory role among leukocytes. Now it refers to a class of cytokines whose molecular structure and biological function have been basically clarified, and which have an important regulatory role and are named uniformly. It belongs to cytokines like blood cell growth factor. The two coordinate and interact with each other to complete hematopoietic and immune regulation functions. Interleukins play an important role in transmitting information, activating and regulating immune cells, mediating T and B cell activation, proliferation and differentiation, and in inflammatory responses. [0003] Interleukin-6 is a pleiotropic cytokine that...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/543
CPCG01N33/6869G01N33/577G01N33/54326G01N2333/5412G01N2800/7095
Inventor 董宁张巍
Owner SUZHOU SYM BIO LIFESCI CO LTD
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