New application of 2-amino-2 '-fluoro-2'-deoxyadenosine
A technology of deoxyadenosine and amino group, applied in the field of medicine, can solve problems such as the use that has not been seen in relevant reports
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Embodiment 1
[0028] Example 1 Preparation of 2-amino-2'-fluoro-2'-deoxyadenosine (2-FA) hydrogel and its mechanical strength In this example, 2-FA hydrogel was prepared by the following method:
[0029] Step 1, prepare 1.7wt%, 2.5wt%, 5.0wt% 2-FA aqueous solution;
[0030] Step 2, heating to 100°C to obtain a clear and transparent solution;
[0031] Step 3, naturally cooling the solution at room temperature to obtain milky white 2-FA hydrogel. The appearance of the prepared 2-FA hydrogel is as follows figure 1 As shown in h-i (where figure 1 The sample of h also added ThT or methylene blue in step 1 for the convenience of observation).
[0032] The vial with the above-mentioned 2-FA hydrogel was inverted to observe its fluidity, and the results were as follows: figure 1 As shown in a-b, different concentrations of 2-FA can self-assemble to form supramolecular hydrogels, which have no fluidity. Among them, 2-FA at a concentration of 5 wt% remained in a stable solid state for 10 months....
Embodiment 2
[0037] Example 2 Shear thinning injectability of 2-FA hydrogel
[0038] In this example, a rheological test is used to evaluate the self-healing ability of the 2-FA hydrogel prepared in Example 1, so as to study its injectability.
[0039] figure 2 a–b show the changes in the modulus of 2-FA hydrogels under alternating strain changes. It can be seen that with the change of alternating strain, the modulus of 2-FA hydrogel can always return to its initial value. At the same time, the hydrogel showed good shear thinning properties ( figure 2 c).
[0040] figure 2 d-g show that when the injectable experiment is carried out in vitro, the hydrogel gels in the needle tube, and becomes writable after being shear-thinned into a liquid by needle injection. Simultaneously, inject in vial, 2.5wt% hydrogel can become gel after 18min, 5.0wt% hydrogel then can become gel immediately ( figure 2 e-g).
[0041] The above results show that 2-FA hydrogels can be shear-thinned and can q...
Embodiment 3
[0042] Example 3 In vitro biocompatibility of 2-FA
[0043] This example uses live / dead cell staining and alarm Blue detection to evaluate the biocompatibility of the 2-FA hydrogel prepared in Example 1 applied to tooth extraction wounds.
[0044] The specific method is: after digestion, resuspension and counting, 5×10 4 Each / mL MC3T3-E1 or RAW 264.7 cells were seeded in 24-well plates, 1 mL per well. After the cells adhered to the wall, the liquids of the control group and the experimental group were added to each well. Set PBS as the control group, and 0.05mM, 0.1mM, 1mM, 2.5mM, 5mM 2-FA as the experimental group. The above cells were collected at 1, 2, and 3 days of culture, and live / dead cell staining and alarm Blue detection were performed. Live / dead cell staining was performed using a live / dead cell staining kit (Calcein AM, PI method). According to the reagent instructions, two widely used fluorescent probes, Calcein AM and PI, were used to stain the cells. Live cel...
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