Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

New application of 2-amino-2 '-fluoro-2'-deoxyadenosine

A technology of deoxyadenosine and amino group, applied in the field of medicine, can solve problems such as the use that has not been seen in relevant reports

Active Publication Date: 2022-03-01
SICHUAN UNIV
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no relevant report on the use of 2-amino-2'-fluoro-2'-deoxyadenosine in tooth extraction wound packing materials

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • New application of 2-amino-2 '-fluoro-2'-deoxyadenosine
  • New application of 2-amino-2 '-fluoro-2'-deoxyadenosine
  • New application of 2-amino-2 '-fluoro-2'-deoxyadenosine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of 2-amino-2'-fluoro-2'-deoxyadenosine (2-FA) hydrogel and its mechanical strength In this example, 2-FA hydrogel was prepared by the following method:

[0029] Step 1, prepare 1.7wt%, 2.5wt%, 5.0wt% 2-FA aqueous solution;

[0030] Step 2, heating to 100°C to obtain a clear and transparent solution;

[0031] Step 3, naturally cooling the solution at room temperature to obtain milky white 2-FA hydrogel. The appearance of the prepared 2-FA hydrogel is as follows figure 1 As shown in h-i (where figure 1 The sample of h also added ThT or methylene blue in step 1 for the convenience of observation).

[0032] The vial with the above-mentioned 2-FA hydrogel was inverted to observe its fluidity, and the results were as follows: figure 1 As shown in a-b, different concentrations of 2-FA can self-assemble to form supramolecular hydrogels, which have no fluidity. Among them, 2-FA at a concentration of 5 wt% remained in a stable solid state for 10 months....

Embodiment 2

[0037] Example 2 Shear thinning injectability of 2-FA hydrogel

[0038] In this example, a rheological test is used to evaluate the self-healing ability of the 2-FA hydrogel prepared in Example 1, so as to study its injectability.

[0039] figure 2 a–b show the changes in the modulus of 2-FA hydrogels under alternating strain changes. It can be seen that with the change of alternating strain, the modulus of 2-FA hydrogel can always return to its initial value. At the same time, the hydrogel showed good shear thinning properties ( figure 2 c).

[0040] figure 2 d-g show that when the injectable experiment is carried out in vitro, the hydrogel gels in the needle tube, and becomes writable after being shear-thinned into a liquid by needle injection. Simultaneously, inject in vial, 2.5wt% hydrogel can become gel after 18min, 5.0wt% hydrogel then can become gel immediately ( figure 2 e-g).

[0041] The above results show that 2-FA hydrogels can be shear-thinned and can q...

Embodiment 3

[0042] Example 3 In vitro biocompatibility of 2-FA

[0043] This example uses live / dead cell staining and alarm Blue detection to evaluate the biocompatibility of the 2-FA hydrogel prepared in Example 1 applied to tooth extraction wounds.

[0044] The specific method is: after digestion, resuspension and counting, 5×10 4 Each / mL MC3T3-E1 or RAW 264.7 cells were seeded in 24-well plates, 1 mL per well. After the cells adhered to the wall, the liquids of the control group and the experimental group were added to each well. Set PBS as the control group, and 0.05mM, 0.1mM, 1mM, 2.5mM, 5mM 2-FA as the experimental group. The above cells were collected at 1, 2, and 3 days of culture, and live / dead cell staining and alarm Blue detection were performed. Live / dead cell staining was performed using a live / dead cell staining kit (Calcein AM, PI method). According to the reagent instructions, two widely used fluorescent probes, Calcein AM and PI, were used to stain the cells. Live cel...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of medicines, and particularly relates to a novel application of 2-amino-2 '-fluoro-2'-deoxyadenosine. According to the invention, the application of the 2-amino-2 '-fluoro-2'-deoxyadenosine or the hydrogel formed by the 2-amino-2 '-fluoro-2'-deoxyadenosine to the preparation of the tooth extraction wound packing material is proposed for the first time. The 2-amino-2 '-fluoro-2'-deoxyadenosine or the hydrogel formed by the 2-amino-2 '-fluoro-2'-deoxyadenosine has the characteristics of rapid degradation, inhibition of activities of macrophages and osteoclasts and antibacterial property, so that the 2-amino-2 '-fluoro-2'-deoxyadenosine can promote healing of tooth extraction wounds. The 2-amino-2 '-fluoro-2'-deoxyadenosine hydrogel disclosed by the invention can be used for preparing a tooth extraction wound packing material and has a very good application prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a new application of 2-amino-2'-fluoro-2'-deoxyadenosine. Background technique [0002] Tooth extraction is one of the most important operations in oral and maxillofacial surgery, and its main purpose is to extract impacted teeth, teeth with pulp, periodontal disease, and orthodontic teeth for the purpose of prevention or treatment. Impacted teeth are the most common indications, among which the incidence of impacted third molars is 18%-32% higher. However, some complications are common after tooth extraction, including dry socket (0.5%-32.5%), infection of extraction wound (0.9%-4.2%), and bleeding (0.2%-1.5%). [0003] At present, the commonly used clinical filling materials for tooth extraction wounds include gelatin sponge and oxidized cellulose. Both have the effect of promoting blood clot formation and accelerating hemostasis. However, these filling material...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/7076A61K9/06A61P1/02
CPCA61K31/7076A61K9/06A61K9/0024A61P1/02
Inventor 赵行尹一佳王峥韩向龙陈谦明
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com