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Efficient column chromatography system

A chromatography system and high-efficiency column technology, applied in separation methods, chemical instruments and methods, solid adsorbent liquid separation, etc., can solve the problems that column chromatography equipment cannot realize automatic operation, low purity and poor stability of the obtained products, etc. Achieve the effects of saving labor costs and time costs, avoiding manual replacement of chromatography columns, and convenient operation

Pending Publication Date: 2022-03-01
LUNAN PHARMA GROUP CORPORATION
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the common technical problems that the existing column chromatography equipment cannot realize automatic operation, long time consumption, low yield, poor stability and low purity of the obtained product, the technical problem to be solved by the present invention is to provide an automatic loading and elution High efficiency column chromatography system

Method used

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  • Efficient column chromatography system
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Effect test

Embodiment 1

[0053] Embodiment 1 High-efficiency column chromatography system structure and operation mode

[0054] as attached figure 1 As shown, a high-efficiency column chromatography system of the present invention is mainly composed of a chromatography column 3, a deacidification column 4, a liquid storage unit 10, a temporary storage unit 20, and a collection unit 30. The liquid storage unit 10 passes through the first high-pressure The constant flow pump inlet pipe 101 communicates with the first high-pressure constant-flow pump 1, and the first high-pressure constant-flow pump 1 communicates with the chromatography column 3 through the first high-pressure constant-flow pump outlet pipe 102, and the chromatography column 3 passes through the first online The detector liquid inlet pipe 201 communicates with the first online detector 5, and the first online detector 5 communicates with the temporary storage unit 20 through the first online detector liquid outlet pipe 202, and the temp...

Embodiment 2

[0062] Example 2 The research on the purification of Ginkgo biloba extract by column chromatography integrated system

[0063] 1 material

[0064]Ginkgo biloba extract was provided by Lunan Houpu Pharmaceutical Co., Ltd. Drug Research and Development Center (Ginkgo biloba was purchased from Shandong Tancheng Shengqiang Ginkgo Co., Ltd.); LX-158 and LX-02 macroporous resins (Xi’an Lanxiao Technology New Materials Co., Ltd. ); 36×920mm protein compression column and 200mL microfluidic reactor pump (Shanghai Saifanke Separation Technology Co., Ltd.).

[0065] 2 methods

[0066] 2.1 Purification of Ginkgo biloba extract by conventional column chromatography equipment

[0067] Column packing: Take a clean protein compression column and pack it tightly and evenly with LX-158 macroporous resin (column volume is about 540mL), (this step can be omitted for regenerating the chromatography column).

[0068] Column flushing: set the pump flow rate of the microflow reactor to 35mL / min, ...

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Abstract

The invention discloses an efficient column chromatography system, and belongs to the field of medical equipment. The device mainly comprises a chromatographic column (3), a deacidification column (4), a liquid storage unit (10), a temporary storage unit (20) and a collection unit (30), wherein the liquid storage unit (10), a first high-pressure constant flow pump (1), the chromatographic column (3), a first online detector (5), the temporary storage unit (20), a second high-pressure constant flow pump (2), the deacidification column (4), a second online detector (6) and the collection unit (30) are communicated through communicating pipes. The device is scientific in structure, convenient to operate and wide in application range, automatic sample loading, elution and multi-step dynamic continuous operation can be achieved, the chromatography efficiency and the yield and purity of target products are greatly improved, labor and materials are saved, and the device has wide application and popularization prospects.

Description

technical field [0001] The invention relates to a high-efficiency column chromatography system, which is suitable for the separation and purification of active ingredients of traditional Chinese medicine and natural medicine, and belongs to the field of medical equipment. Background technique [0002] Column chromatography is a separation and purification technology widely used in biology, medicine, food and other fields. It is a technical means to separate the mixture according to the different distribution coefficients of the components in the mixture in the stationary phase and mobile phase. Depending on the mechanism of column chromatography, it also includes various forms such as adsorption chromatography, partition chromatography, ion exchange chromatography, and gel filtration chromatography. However, in the application process of existing column chromatography, there are common problems such as low column utilization efficiency, poor stability, and low production cap...

Claims

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Application Information

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IPC IPC(8): B01D15/10
CPCB01D15/10
Inventor 郝大伟关永霞冯巧巧许辉
Owner LUNAN PHARMA GROUP CORPORATION
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