Method for preparing collagen with triple-helix structure through spray drying

A collagen and spray dryer technology, applied in the field of protein preparation, can solve problems such as restricting the use in the field of bio-3D printing, and achieve the effects of good resolubility, reduced energy consumption, and good storage stability

Active Publication Date: 2022-03-01
GUANGZHOU TRAUER BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing collagen powder is difficult to reconstitute under neutral conditions, which limits its use in the field of 3D bioprinting

Method used

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  • Method for preparing collagen with triple-helix structure through spray drying
  • Method for preparing collagen with triple-helix structure through spray drying
  • Method for preparing collagen with triple-helix structure through spray drying

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The collagen stock solution used is prepared from pig Achilles tendon with a purity of 99.8%, a concentration of 8 mg / mL, and an rpn value of triple helix structure integrity of 0.12;

[0061] Spray drying: the inlet air temperature is 60±1°C, the feed speed is 30rpm, the outlet air temperature is 50-58°C, and the obtained powder is collected.

Embodiment 2

[0069] The collagen stock solution used is prepared from bovine Achilles tendon with a purity of 99.8%, a concentration of 15 mg / mL, and an rpn value of triple helix structure integrity of 0.12;

[0070] Spray drying: inlet air temperature: the inlet air temperature is 50±1°C, the feed speed is 25 rpm, the outlet air temperature is 30-40°C, and the obtained powder is collected.

Embodiment 3

[0078] The collagen stock solution used was prepared from rat tail with a purity of 99.8%, a concentration of 20 mg / mL, and an rpn value of triple helix structure integrity of 0.12;

[0079] Spray drying: the inlet air temperature is 40±1°C, the feed speed is 18 rpm, the outlet air temperature is 20°C-30°C, the obtained powder is collected, and sterilized by irradiation.

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Abstract

The invention belongs to the field of protein preparation, and discloses a method for preparing collagen with a triple-helix structure through spray drying. In the research process, the inventor finds that the triple helix structure of the collagen is basically not damaged by controlling the collagen stock solution to be subjected to spray drying under specific conditions, the collagen obtained by drying is powdery, the particle size is concentrated at 1-2.5 microns, the water content is lower than 15%, and the collagen has better storage stability and good redissolvability. The collagen stock solution obtained by redissolving keeps a triple helix structure, the content of hydroxyproline is not less than 9%, and the biological activity of the collagen stock solution is basically not reduced compared with that of the collagen stock solution before spray drying. The collagen powder prepared by some examples of the invention meets the injection-grade requirement. In some examples of the invention, the bio-ink of a 3D printing material can be prepared by adding the matrix strength enhancer.

Description

technical field [0001] The invention belongs to the field of protein preparation, and relates to a drying process of collagen, in particular to a method for preparing collagen with a triple helix structure by spray drying. Background technique [0002] Collagen is a family of secreted proteins containing at least 28 genetically different types. It mainly serves as the structural support function of the body, has a unique triple helical configuration, and has certain biological functions. Collagen can realize biological functions by interacting with various cell membrane receptors and cytokines through the domains on its triple helix structure, regulate cell adhesion, migration, signal transduction and proliferation, and have a strong repair effect. Therefore, the loss of collagen triple helix structure will lead to the loss of some of its biological activity. [0003] Collagen has good biocompatibility and unique biological functions. It is a biomedical material that has be...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/14
CPCC07K14/78
Inventor 陈淡嫦殷率杨剑易静楠叶春婷
Owner GUANGZHOU TRAUER BIOTECH
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