Application of rabdosia lophanthide in prevention or treatment of alcohol dependence
A technology of alcohol dependence and Xihuangcao, which is applied in the field of biomedicine to achieve the effect of reducing alcohol preference, improving anxiety-like behavior, and preventing alcohol dependence
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Embodiment 1
[0062] Preparation of the water extract of Xihuangcao: Take 100g of dried Xihuangcao whole herb, add 600ml of water to decoct twice, each decocting time is 1h, combine the decoctions, and concentrate to a thick consistency with a relative density of 1.1-1.25 (50-60°C). Ointment, standby (1g Xihuangcao crude drug is equivalent to 0.1g Xihuangcao extract).
[0063] 1. Experimental reagents
[0064] Alcohol: (intraperitoneal injection dose 2g / kg, concentration 15%);
[0065] 0.9% normal saline;
[0066] The water extract of Xihuangcao prepared in Example 1 (modeling is intraperitoneal injection, treatment is intragastric administration).
[0067] 2. Experimental animals and grouping
[0068] C57BL / 6J mice, male, 150, at least 60 mice that can be used for the experiment were screened out by conditional place preference test (CPP), divided into 6 groups, 10 in each group; followed by normal control group (normal saline) , simple Xihuangcao group (Xihuangcao crude drug 1.0g / kg+n...
Embodiment 2
[0103] The preparation method of the ethanol extract of Xihuangcao: take a certain amount of Xihuangcao, add 20 times the amount of 70% ethanol, extract for 25 minutes, filter, and concentrate under reduced pressure.
Embodiment 3
[0105] The present embodiment provides a product for preventing and treating alcohol dependence. The preparation method is as follows: take 100g of the whole herb dry product of Xihuangcao and 100g of sweet-scented osmanthus (dried product), add 1000ml of water to decoct twice, each decoction time is 1h, and the decoctions are combined. Concentrate, that is.
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