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Silencing method of aleyrodid carotenoid synthetic gene

A carotene and gene synthesis technology, which is applied in the field of whitefly carotenoid synthesis gene silencing, and the field of silencing the whitefly carotenoid synthesis gene affecting the yellowing of Bemisia tabaci, which can solve the problems of interfering with the visual ability of insects and the like

Active Publication Date: 2022-03-04
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, by interfering with the visual ability of insects, it can provide a theoretical basis for the pollution-free control of pests, but there is currently no method for controlling whitefly based on affecting the visual ability of whiteflies.

Method used

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  • Silencing method of aleyrodid carotenoid synthetic gene
  • Silencing method of aleyrodid carotenoid synthetic gene
  • Silencing method of aleyrodid carotenoid synthetic gene

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Experimental program
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Embodiment Construction

[0031] Silencing the carotenoid synthesis gene in Bemisia tabaci affects the yellowing of Bemisia tabaci:

[0032] 1. Obtain the 2mDNA1 silencing expression vector

[0033] 1.1 Acquisition of B. tabaci gene target fragments

[0034] The gene target fragment Bt GGPS1 Has the nucleotide sequence shown in SEQ ID NO: 1:

[0035] atggatgtga acggctgtgt ctacaaaagc aaaactggcg ataaggatca agatgaaatt

[0036] ttactgcaac ccttcacgta catcctgcaa gttccaggaa agcaaatcag aggaaaatta

[0037] gcgcaggctt tcaatgtttg gctaaatatt ccagatgaga agttggccgt tgttggggat

[0038] attgttttgt tgctgcacaa ctccagtttg ttaattgatg acattcaaga taactcaatt

[0039] ttaaggagg gcatccctgt tgctcattct atctacggag tggccagcac catcaatgcc

[0040]gctaattatg ttctttttat tgcccttgaa agggtgctca gccttggtca tccagaggca

[0041] acgtctgtgt atacagaaca gctcttgaag ttgcaccgag gacagggcat ggaaatttat

[0042] tggcgagaca attatctatg tccttccgaa gaagaatacc gaactatgac tatgtgtaaa

[0043] actggtggat tattcatgct tgctatcagg ttgatgcagc tctttagtga c...

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Abstract

The invention relates to a aleyrodid carotenoid synthetic gene silencing method which comprises the following steps: (1) constructing a silencing expression vector to obtain agrobacterium containing a 2mDNA1-GGPS1 silencing expression vector; (2) inoculating a healthy plant with the agrobacterium containing the 2mDNA1-GGPS1 silent expression vector according to an agrobacterium infection method, and carrying out 2mDNA1 specific detection to obtain a VIGS plant carrying the 2mDNA1 silent vector; (3) feeding aleyrodid on the VIGS plant carrying the 2mDNA1 silencing vector; the aleyrodid with the 2mDNA1-GGPS1 silent expression vector is obtained, the expression of the carotenoid synthetic gene GGPS1 of the aleyrodid with the 2mDNA1-GGPS1 silent expression vector is inhibited, and the carotenoid content is reduced; the method is used for interfering the visual ability of the aleyrodid and achieving the aim of preventing and controlling the aleyrodid.

Description

technical field [0001] The invention relates to the control of agricultural insects and pests, in particular to a method for silencing whitefly carotenoid synthesis gene, that is, a method for silencing the carotenoid synthesis gene of Bemisia tabaci to influence the yellowing of Bemisia tabaci. Background technique [0002] Bemisia tabaci are tiny insects belonging to the order Hemiptera, the family Albaeidae and the genus Bacillus. With the rapid development of global trade, Bemisia tabaci is widely spread around the world, damaging economic crops such as vegetables and flowers, as well as food crops such as cassava and sweet potato. Bemisia tabaci can harm host plants in both the adult and nymph stages. cause significant economic losses. [0003] At present, the control of Bemisia tabaci is mainly dependent on chemical control in production practice. However, due to the abuse and irregular use of chemical pesticides, the problem of whitefly resistance is becoming more an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/84C12N15/66C12N15/54A01H5/00A01H6/82C12N15/85A01K67/033
CPCC12N15/8205C12N15/8286C12N9/1085C12N15/8509A01K67/0339C12Y205/01029C12N2800/105A01K2217/075A01K2227/706
Inventor 黄艳贞栾军波卢月王天玉
Owner SHENYANG AGRI UNIV
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