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Swine atrophic rhinitis inactivated vaccine and preparation method thereof

A technology for atrophic rhinitis and inactivated vaccines, which is applied in the field of porcine atrophic rhinitis inactivated vaccines and its preparation, can solve the problems of poor versatility of toxins, and achieve the effects of improving immunogenicity, reducing incomplete absorption, and shortening production time

Pending Publication Date: 2022-03-08
JIANGSU NANNONG HI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the defect of existing vaccine, provides a kind of porcine atrophic rhinitis inactivated vaccine and preparation method thereof, to solve the technical problem that relevant vaccine in the prior art is poor to different strains and the toxin that produces

Method used

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  • Swine atrophic rhinitis inactivated vaccine and preparation method thereof
  • Swine atrophic rhinitis inactivated vaccine and preparation method thereof
  • Swine atrophic rhinitis inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Preparation of porcine atrophic rhinitis inactivated vaccine

[0035] 1 Source and identification of the bacterial species in the embodiment of the present invention

[0036] 1.1 Source of strains

[0037] Porcine toxin-producing Pasteurella multocida ZXT+Pm strain deposit number CCTCC M 20211427

[0038] ZXT-Pm, Bordetella bronchiseptica AHBb strain with deposit number CCTCC M 20211379 were isolated, identified, stored and supplied by the Key Laboratory of Bacteriology, Ministry of Agriculture, Nanjing Agricultural University. The recombinant expression strain Rosetta-rPMT-N deposit number CCTCC M 20211371, and the recombinant expression strain Rosetta-rPMT-C deposit number CCTCC M 20211372 were constructed, identified, kept and supplied by the Key Laboratory of Bacteriology, Ministry of Agriculture, Nanjing Agricultural University.

[0039] 1.2 Identification of strains

[0040] 1.2.1 Porcine D-type toxin-producing Pasteurella multocida strain ZXT+Pm

[...

Embodiment 2

[0072] The preparation of embodiment 2 porcine atrophic rhinitis inactivated vaccine 2

[0073] The strain and the inactivation of the strain, the expression and purification of rPMT-N and rPMT-C are the same as in Example 1.

Embodiment 3

[0074] The preparation of embodiment 3 porcine atrophic rhinitis inactivated vaccine 3

[0075] The strain and the inactivation of the strain, the expression and purification of rPMT-N and rPMT-C are the same as in Example 1.

[0076] The ratio of each antigen in Example 2 and Example 3 is shown in Table 1 below.

[0077] Table 1 The composition ratio of porcine atrophic rhinitis inactivated vaccine

[0078]

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Abstract

The invention discloses a swine atrophic rhinitis inactivated vaccine and a preparation method thereof. The vaccine is prepared by the following steps: mixing swine toxicant-producing pasteurella multocida, swine bordetella bronchiseptica, toxicant-producing pasteurella multocida PMT toxin N-terminal and C-terminal proteins with a carbomer water adjuvant in a certain proportion; the vaccine has good immunogenicity, can effectively prevent progressive and non-progressive atrophic rhinitis of pigs, and improves the productivity of pigs. The swine atrophic rhinitis inactivated vaccine prepared by the invention contains ZXT + Pm and AHBb antigen components, and rPMT-N and rPMT-C are added, so that swine atrophic rhinitis caused by ZXT + Pm and AHBb is prevented, osteolysis generated by toxin protein secreted by ZXT + Pm is also avoided, and the immune protection effect on swine atrophic rhinitis is enhanced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an inactivated vaccine for porcine atrophic rhinitis and a preparation method thereof. Background technique [0002] Atrophic rhinitis (Progressive atrophic rhinitis, PAR) is a type of chronic respiratory disease, characterized by extensive expansion of the nasal cavity and atrophy of mucosa, submucosa and bone tissue, and is highly contagious, also known as infectious atrophic rhinitis or chronic atrophic rhinitis. Atrophic rhinitis is recognized as one of the important infectious diseases that seriously endanger the production and development of intensive pig farms. PAR is mainly caused by the infection of toxin-producing Pasteurella multocida and Bordetella bronchiseptica, and often occurs secondary infection with other respiratory diseases, such as swine mycoplasma disease, pig blue ear disease, swine panting disease, etc. The disease has a wide range of epidemics and occurs gen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/102A61K39/02A61K39/39A61P11/02A61P29/00
CPCA61K39/102A61K39/0208A61K39/39A61P11/02A61P29/00A61K2039/55511A61K2039/521A61K2039/552A61K2039/70Y02A50/30
Inventor 董彦鹏李玉峰胡静雅肖澄周琳余姣靳蒙蒙车巧林巫泉
Owner JIANGSU NANNONG HI TECH
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