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IncRNA (long non-coding ribonucleic acid) for regulating and controlling soluble sugar content of plant and application of lncRNA

A sugar content, soluble technology, applied in the field of molecular biology, can solve the problem that the function of lncRNA needs to be studied and so on

Pending Publication Date: 2022-03-08
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of lncRNAs, especially the functions of lncRNAs related to plant metabolism, need to be studied

Method used

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  • IncRNA (long non-coding ribonucleic acid) for regulating and controlling soluble sugar content of plant and application of lncRNA
  • IncRNA (long non-coding ribonucleic acid) for regulating and controlling soluble sugar content of plant and application of lncRNA
  • IncRNA (long non-coding ribonucleic acid) for regulating and controlling soluble sugar content of plant and application of lncRNA

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Experimental program
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Effect test

Embodiment 1

[0057] Embodiment 1, the research of MtCIR2 gene

[0058] In order to reveal the molecular basis of alfalfa metabolism from the level of non-coding RNA, the inventors used high-throughput sequencing technology and bioinformatics analysis methods to identify the lncRNA of Medicago truncatula. It was found that the lncRNA MtCIR2 gene of alfalfa can promote the synthesis of soluble sugar in plants.

[0059] 1. Construction of MtCIR2 expression vector

[0060] 1.1 Cloning of MtCIR2 gene

[0061] Genomic DNA of wild type Medicago truncatula was extracted by CTAB method.

[0062] Using the wild-type DNA of Medicago truncatula as a template, Takara Prime HSDNA polymerase for MtCIR2 gene amplification. MtCIR2 gene (the nucleotide sequence is as shown in sequence 1 in the sequence listing, and the long-chain non-coding RNA whose coding sequence is shown in sequence 2 in the sequence listing) amplification primers are as follows:

[0063] Forward Primer: 5′-TC GGCGCGCC AGGCATAAT...

Embodiment 2

[0168] Example 2, Application of MtCIR2 Gene in Regulating Plant Soluble Sugar Synthesis

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Abstract

The invention discloses a long-chain non-coding RNA (Ribonucleic Acid) named as MtCIR2. The long-chain non-coding RNA is shown as A1) or A2): A1) a long-chain non-coding RNA of which the nucleotide sequence is a sequence 2 in a sequence table; a2) a long-chain non-coding RNA which is obtained by substitution and / or deletion and / or addition of one or more nucleotides of the nucleotide sequence shown in the sequence 2 in the sequence table, has 90% or more of identity with the long-chain non-coding RNA shown in A1) and is related to the content of soluble sugar in the plant. Transgenosis experiments of transferring the MtCIR2 gene into medicago truncatula R108, medicago sativa, medicago falcata and arabidopsis col-0 prove that compared with a receptor plant, the soluble sugar content of a transgenic plant expressing the MtCIR2 gene is remarkably improved, which indicates that the MtCIR2 gene is a gene related to the soluble sugar content of the plant.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to an lncRNA for regulating plant soluble sugar content and application thereof. Background technique [0002] Legumes are important economic, energy and food crops. However, most of the cultivated leguminous plants have large genomes and complex structures, lack of effective genetic transformation systems, and are difficult to carry out molecular biological analysis. The annual Medicago truncatula has a close genetic relationship with most legumes, and has the advantages of diploid homozygosity, small genome, short growth cycle, easy artificial propagation and gene transformation, and genome sequencing, making it a good candidate for molecular biology research in legumes. Pattern plants. [0003] Genome and transcriptome sequencing studies have found that more than 75% of the human genome and more than 50% of the Arabidopsis genome can be transcribed into RN...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/82A01H5/00A01H6/20A01H6/54
CPCC12N15/113C12N15/8245
Inventor 张文浩赵敏桂任立飞田睿孙晓涵
Owner INST OF BOTANY CHINESE ACAD OF SCI
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