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Application of tetrahedral framework nucleic acid in medicine for treating epilepsy

A tetrahedron, drug technology, applied in the field of biomedicine, can solve problems such as side effects, singleness, and patient depression

Pending Publication Date: 2022-03-11
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These drugs can indeed achieve better clinical effects, but the mechanism and target of drug action are mostly concentrated on neurons, and they are relatively single, often requiring combination drugs and long-term medication. Drug tolerance and related side effects, and long-term administration of these drugs to varying degrees inhibit the excitability of the central nervous system, leading to depression or suicide in patients is rarely reported

Method used

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  • Application of tetrahedral framework nucleic acid in medicine for treating epilepsy
  • Application of tetrahedral framework nucleic acid in medicine for treating epilepsy
  • Application of tetrahedral framework nucleic acid in medicine for treating epilepsy

Examples

Experimental program
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Embodiment 1

[0036] Embodiment 1, the synthesis of DNA tetrahedron

[0037] Dissolve the four DNA single strands (S1, S2, S3, S4) in TM Buffer (10mM Tris-HCl, 50mM MgCl2, pH=8.0), the final concentration of the four DNA single strands is 1μM, mix well, and rapidly heat to 95 Keep the temperature at ℃ for 10 minutes, then quickly cool down to 4℃ and keep it for more than 20 minutes to obtain tetrahedral framework nucleic acid.

[0038] The sequences of the four single strands are as follows:

[0039]

[0040]

[0041] Synthesized tetrahedral framework nucleic acid, PAGE gel electrophoresis ( figure 1 ) and capillary electrophoresis ( figure 2 ) It can be seen that the size of the tetrahedral framework nucleic acid is about 180KD, and it can be considered that the tetrahedral framework nucleic acid has been successfully synthesized. Tetrahedral particle size is about 20.12nm ( image 3 ), the potential is -6.29mV ( Figure 4 ). Using a transmission electron microscope, the tetra...

experiment example 1

[0043] Experimental example 1. Therapeutic effect of tetrahedral framework nucleic acid on demyelinating disease model

[0044] 1. Experimental method

[0045]Model animals: Use Pilocarpine to induce ICR mice to prepare a model of status epilepticus, and explore the effect and possible mechanism of tetrahedral framework nucleic acid regulating gliosis, neuroinflammation, and neuronal excitability. A total of 38 10-week-old ICR male mice were taken. Divided into normal group 9, tetrahedron control group 9, model group 10, treatment group 10. The treatment group started to inject tetrahedral framework nucleic acid (1000nM) into the tail vein 4 hours after the seizure, once every 24 hours, for 14 consecutive days. The tetrahedron control group was given an equal dose of tetrahedral framework nucleic acid for daily injection, and the normal group and model group were given an equal dose of normal saline for daily injection.

[0046] Detection of related indicators: On the secon...

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Abstract

The invention provides application of tetrahedral framework nucleic acid in a medicine for treating epilepsy. The tetrahedral framework nucleic acid is formed by four single-stranded DNAs through base complementary pairing; the sequences of the four single-stranded DNAs are respectively selected from the sequences as shown in SEQ ID NO.1-4 in a one-to-one manner. The tetrahedral framework nucleic acid can inhibit down-regulation of glutamine synthetase in inflammatory glial cells by relieving proliferation of glial cells in a brain hippocampus and neuroinflammation caused by release of inflammatory factors, so that metabolism of excitatory glutamic acid is facilitated, and accumulation of glutamic acid and nervous excitability caused by accumulation of glutamic acid are relieved. Therefore, the tetrahedral framework nucleic acid can effectively control abnormal discharge of epilepsy and has potential clinical application prospects.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the use of tetrahedral framework nucleic acid in drugs for treating epilepsy. Background technique [0002] Epilepsy is a kind of nervous system disease whose main clinical manifestation is involuntary twitching or twisting of all or part of the limbs caused by abnormal discharge of neurons. In severe cases, it is often accompanied by loss of consciousness, and even endangers the life of the patient. Most patients often turn to epilepsy. Patients with chronic epilepsy have seriously affected their daily work, life and social communication. At present, there are about 7 million epilepsy patients in the world, and their treatment is mainly based on drug therapy. However, about 1.5 million epilepsy patients have tolerance or poor response to the existing antiepileptic drugs, and finally have to undergo surgery . At the same time, long-term medication of antiepileptic drugs n...

Claims

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Application Information

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IPC IPC(8): A61K31/7088A61P25/08
CPCA61K31/7088A61P25/08
Inventor 林云锋蔡潇潇
Owner SICHUAN UNIV
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