Preparation method of 18-alpha glycyrrhetinic acid

Abstract

The invention discloses a preparation method of 18-alpha glycyrrhetinic acid, which comprises the following steps: by taking licorice decoction pieces as a raw material, firstly carrying out enzymolysis treatment by using a compound enzyme, then decocting and concentrating to obtain a licorice concentrated solution rich in glycyrrhizic acid, and then carrying out enzymolysis under the catalytic action of a culture product of a bacillus subtilis subsp. Natto strain of a high-yield glucuronidase enzyme system to obtain the 18-alpha glycyrrhetinic acid. Continuously converting glycyrrhizic acid in the liquorice concentrated solution into 18-beta glycyrrhetinic acid to obtain a reaction solution; a culture product of a rhizopus oryzae strain of a high-yield glycyrrhetinic acid isomerase enzyme system is compounded with catalase to prepare a rhizopus oryzae mycelium-catalase co-immobilized cell catalyst, under the catalytic action of the rhizopus oryzae mycelium-catalase co-immobilized cell catalyst, the reaction liquid reacts to generate a crude product of 18-alpha glycyrrhetinic acid, and the crude product is purified to obtain the 18-alpha glycyrrhetinic acid. On the premise that the liver protection curative effect of the glycyrrhetinic acid is reserved, the side effects of 18-beta glycyrrhetinic acid caused by long-term application are removed, so that the 18-beta glycyrrhetinic acid can be safely and effectively applied to clinical treatment of various liver diseases, and the safety of clinical medication is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a preparation method of 18-alpha glycyrrhetinic acid. Background technique [0002] Glycyrrhiza licorice is a kind of food and medicine dual-purpose plant. It is widely used in food, feed, cosmetics and daily chemicals as flavoring agent, thickener, auxiliary agent, additive, preservative, antioxidant, etc., but its The most important use is in the field of medicine. [0003] There is a saying in Chinese medicine that there are "ten directions and nine herbs", that is, most of the compound prescriptions of Chinese medicine are inseparable from licorice, and it is known as the "national guy" of Chinese medicine; the main chemical component of licorice, glycyrrhizic acid and its active component, glycyrrhetinic acid, It is widely used in Chinese patent medicine and modern Chinese medicine. It is particularly pointed out that among the traditional Chinese medicine compounds a...

AI Technical Summary

Problems solved by technology

[0004] Unfortunately, the active pharmaceutical ingredient of glycyrrhizic acid (pentacyclic triterpene glycosides), the main liver-protecting active substance in various preparations of licorice, is 18-β glycyrrhetinic acid (glycon), although it has significant and stable However, if it is used for a long time, it will cause side effects such as high blood pressure, hypokalemia, sodium and water retention (collectively referred to as the biological activity of adrenocorticotropic hormone [ACTH]), and even lead to serious conditions such as heart failure, which is life-threatening. Thus limiting its clinical application in chronic liver disease

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