Method for producing alginate lyase through co-fermentation of halophilic lactic acid bacteria and marine bacteria

A technology of alginate lyase and marine bacteria, which is applied in the field of marine biology, can solve the problems of high industrialization cost, complex production process, and long fermentation cycle, and achieve the effects of low cost, short fermentation time, and simple process operation

Pending Publication Date: 2022-03-25
威海长青海洋科技股份有限公司
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  • Application Information

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Problems solved by technology

[0006] The purpose of this application is to provide a method for producing alginate lyase by co-fermentation of halophilic lactic acid bacteria and marine bacteria. Complex technical issues with high industrialization costs

Method used

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  • Method for producing alginate lyase through co-fermentation of halophilic lactic acid bacteria and marine bacteria
  • Method for producing alginate lyase through co-fermentation of halophilic lactic acid bacteria and marine bacteria
  • Method for producing alginate lyase through co-fermentation of halophilic lactic acid bacteria and marine bacteria

Examples

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Effect test

Embodiment 1

[0039] (1) Pre-cultivation of the marine bacteria XS1412C strain: inoculate a single colony of the marine bacteria XS1412C strain into the culture medium, and shake and pre-cultivate it at 26° C. for 7 hours to obtain a highly active marine bacteria XS1412C strain.

[0040] The composition of the culture solution is: sodium alginate 2%, potassium dihydrogen phosphate 0.1%, ammonium chloride 0.2%, peptone 5%, and the treatment condition is: adding filtered seawater to dissolve.

[0041](2) Preparation of marine bacterium XS1412C strain fermentation culture medium: inoculate the marine bacterium XS1412C strain cultivated in step (1) into the fermentation medium according to the inoculum size of 3%, and ferment at 30° C. for 36 hours to prepare marine bacterium XS1412C Strain fermentation broth.

[0042] The composition of the fermentation culture liquid is: peptone 5%, glucose 5%, sodium alginate 2%, potassium dihydrogen phosphate 0.1%, ammonium chloride 0.4%, magnesium sulfate ...

Embodiment 2

[0057] The difference between this implementation method and Example 1 is that in step (2), the culture temperature of the fermentation broth of the marine bacteria XS1412C strain is 25° C., and the other steps are the same.

Embodiment 3

[0059] The difference between this implementation method and Example 1 is that in step (2), the culture temperature of the fermentation broth of the marine bacteria XS1412C strain is 35° C., and the other steps are the same.

[0060] see figure 1 and figure 2 , are respectively the enzyme activity change diagram and the growth curve of the bacterial strain in the fermentation broth of the marine bacteria XS1412C bacterial strain under different culture temperatures, as can be seen from the above examples 1, 2, and 3, when the temperature is too low, the bacterial strain grows slowly and the enzyme activity Also lower, when the temperature is too high, although the bacterial strain grows faster in a short period of time, the continuous higher temperature inhibits the growth of the bacterial strain, which in turn affects its enzyme production. is 30°C.

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Abstract

The invention provides a method for producing alginate lyase through co-fermentation of halophilic lactic acid bacteria and marine bacteria, which comprises the following steps: culturing a marine bacteria XS1412C strain in advance, inoculating the XS1412C strain into a fermentation culture solution to prepare an XS1412C strain fermentation culture solution, and culturing halophilic lactic acid bacteria in advance to produce alginate lyase. The method comprises the following steps: inoculating halophilic lactic acid bacteria into an XS1412C strain fermentation culture solution for symbiotic fermentation culture to obtain a symbiotic fermentation culture solution, and finally centrifuging the symbiotic fermentation culture solution to obtain an enzyme solution containing alginate lyase. Halophilic lactic acid bacteria capable of generating beneficial substances through fermentation, promoting growth of strains and improving enzyme production capacity of the strains and XS1412C strains capable of secreting alginate lyase are subjected to symbiotic fermentation culture, secretion of alginate lyase can be promoted, enzyme quality can be guaranteed, enzyme activity can be effectively improved, the fermentation process can be simplified, fermentation time can be shortened, and extraction cost can be reduced. The invention belongs to the technical field of marine organisms.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and relates to a method for producing alginate lyase by co-fermentation of halophilic lactic acid bacteria and marine bacteria. Background technique [0002] Alginate is the sodium salt of alginic acid, that is, sodium alginate, also known as sodium alginate, is a kind of water-soluble acidic polysaccharide widely present in various brown algae. Alginate has a wide range of applications. It is an important component of the cell wall of brown algae. It has the characteristics of large molecular weight, high degree of polymerization, high viscosity and high fibrousness. Therefore, the degree of absorption and utilization by the human body is poor, which greatly limits its use. scope. People use scientific methods to decompose alginate into effective components that can be absorbed and utilized, such as decomposing it into small molecular substances through enzymatic reactions. Alginate ol...

Claims

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Application Information

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IPC IPC(8): C12N9/88C12N1/20C12R1/15
CPCC12N9/88C12Y402/02003C12Y402/02011C12N1/20
Inventor 李洋常丽荣姚艳艳陈晓丽卢龙飞王振华
Owner 威海长青海洋科技股份有限公司
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