25 results about "Halorubrum halophilum" patented technology

The invention discloses a method for recovering fungus expanded halophilic aerobic granular sludge by using iron ions, and belongs to the field of biological sewage treatment. The principle of the method is as follows: by adding iron ions and utilizing different biological effects caused by the iron ions on bacteria and fungi, the proliferation of the bacteria is promoted, and the proliferation of the fungi is inhibited, so that the number of the fungi is reduced, and the recovery of the compact and stable structure of the fungus expansion halophilic aerobic granular sludge is realized. According to the invention, the halophilic aerobic granular sludge on which fungi overgrow can recover compact and compact granules in a short time, so that stable operation of the system and rapid treatment of high-salinity wastewater are ensured. The iron ion concentration utilized by the method does not cause ecological risk or environmental pollution to a sludge treatment system, and the method is low in cost, simple to operate, safe, efficient, stable and reliable.

The invention discloses a method for improving alkalinity and recovering fungal type expanded halophilic aerobic granular sludge, and belongs to the technical field of sewage treatment. According to the basic principle of the method, bicarbonate is added, the alkalinity of inlet water is improved, the substrate competitive capacity of bacteria (autotrophic bacteria) and fungi can be improved by utilizing the alkalinity, the metabolic activity of filamentous fungi is inhibited, the population number of the fungi is remarkably reduced, and the bulking state of halophilic aerobic granular sludge induced by the filamentous fungi is successfully reversed; the recovery of the structure and the function of the decomposed halophilic aerobic granular sludge is realized. In this way, the sludge in which fungi grow in an outbreak mode can be recovered into a particle structure within a short time, and the good performance of sewage treatment is recovered again. Adding bicarbonate to improve alkalinity is a non-toxic and pollution-free chemical regulation mode, and the method is environment-friendly and convenient to operate. The method is remarkable in effect and low in cost.

The application discloses a method for recovering fungal expanded salt-tolerant aerobic granular sludge by using iron ions, and belongs to the field of wastewater biological treatment. The principle of the application is that by adding iron ions, different biological effects caused by the iron ions on bacteria and fungi are utilized, the proliferation of bacteria is promoted, and the proliferation of fungi is inhibited, so that the number of fungi is reduced, and the recovery of the compact and stable structure of the fungal expanded salt-tolerant aerobic granular sludge is realized. The application can make the salt-tolerant aerobic granular sludge with excessive fungal growth recover the compact and dense granules in a short time, and ensure the stable operation of the system and the rapid treatment of high-salinity wastewater. The method utilizes the iron ion concentration which does not cause ecological risks or environmental pollution to the sludge treatment system, and has the advantages of low cost, simple operation, safety, high efficiency, stability and reliability.

This invention discloses a halophilic fast-growing bacillus strain that degrades chlorinated persistent organic pollutants and its applications. The strain is *Celeribacter baekdonensis* TBC005, with accession number GDMCC No:65236. The *Celeribacter baekdonensis* TBC005 of this invention exhibits excellent degradation ability against chlorinated persistent organic pollutants. After 14 days of anaerobic culture, it shows significant degradation of C... 10 H 16 The degradation rates of Cl6, TECP, and TCPP reached 21.5%, 16.7%, and 16.9%, respectively; after 35 days of cultivation, the degradation rates of C... 10 H 16 The degradation rates of Cl6, TECP, and TCPP can reach 50.7%, 59.2%, and 61.3%, respectively, and they can be applied to the environmental pollution control and bioremediation of chlorinated persistent organic pollutants.

The present invention relates to a method for treatment of poly hydroxy alkanoate (PHA) containing post-consumer product, the method comprising contacting a post-consumer product with a polypeptide that can catalyze degradation of the PHA, the contact taking place at a temperature at least 40° C. and in the presence of salt at a concentration of 1 M or greater. In a specific embodiment, the polypeptide is a wild-type PHA depolymerase expressed by a halophilic microorganism or a modified PHA depolymerase that includes one or more single-site mutations as compared to the wild-type PHA depolymerase. In another specific embodiment, the polypeptide comprising a modified poly hydroxy butyrate (PHB) depolymerase comprising one or more single-site mutations as compared to SEQ ID NO: 1, and the modified PHB depolymerase having a solubility of 10 mg / L or greater. The present invention also relates to a host cell transformed to express a polypeptide that catalyzes degradation of a PHA in the presence of salt at a concentration of 1 M or greater, wherein the host cell is selected from an E. coli cell or a halophilic microorganism.

The invention discloses a halophilic genetically engineered bacterium for synthesizing beta-carotene as well as a construction method and application of the halophilic genetically engineered bacterium. The halophilic genetically engineered bacterium H.volcanii is constructed by cloning a lycopene beta-cyclase gene Hma-LCYb coded by a halophilic archaea marisportui genome to an expression vector pTA1228 and then transforming the lycopene beta-cyclase gene Hma-LCYb into a halophilic archaea Haloferx volcanii cell. The engineering bacterium can be used for synthesizing beta-carotene, and the yield of the engineering bacterium is up to 0.26 mg / g of dry cell weight. The halophilic genetically engineered bacterium H.volcanii disclosed by the invention solves the problems of strain function deficiency, insufficient yield and safety in the production of natural beta-carotene, has high efficiency, economical efficiency and environmental protection property, and has remarkable industrial application value.

To provide a novel strain of Tetragenococcus halophilus having superior capability for producing ornithine and citrulline, thereby enabling the efficient production of ornithine and citrulline that are beneficial amino acids, and their application in the manufacture of food compositions, supplements, pharmaceuticals and the like suitable for maintaining or promoting health, including stimulation of growth hormone secretion, regulation of sleep-wake cycles, enhancement of liver function, rejuvenation of fatigued skin, reduction of mental stress, and recovery from physical fatigue.SOLUTION: Provided is a strain of Tetragenococcus halophilus KA12022 (Deposit Number: NITE P-03886).SELECTED DRAWING: Figure 4

The present invention relates to a microbiome composition of the fermentation culture supernatant of the halophilic Bacillus polyfermenticus KMU01 strain, which has anti-obesity effects. It has been confirmed that the fermentation culture supernatant of the strain deposited under accession number KCTC11751BP inhibits fat production and accumulation and reduces blood cholesterol levels. Therefore, the composition can be usefully used as a composition for preventing, treating, or ameliorating obesity; or as a composition for reducing body fat (visceral fat) or blood cholesterol.

The application provides a kind of based on hybrid deep learning architecture Halophilic protein prediction method and system, it is related to bioinformatics analysis technical field, the method comprises: obtaining the protein sequence to be predicted;Use protein language model to extract its global semantic embedding feature;At the same time, based on the specific reduction amino acid alphabet, the sequence is re-encoded, and the reduction dipeptide frequency in three interval modes is counted to generate a local composition feature vector;After splicing two kinds of features, input the hybrid deep learning model composed of one-dimensional convolutional neural network and light weight Transformer encoder in series for processing, and finally output the prediction result by full connection layer.The application significantly improves the accuracy, generalization ability and computing efficiency of halophilic protein identification by fusing multi-scale sequence features and hierarchical hybrid network architecture, and provides an effective tool for intelligent mining of large-scale extreme environment enzyme resources.

The invention discloses a halophilic fast-growing bacillus for degrading chlorinated persistent organic pollutants and application of the halophilic fast-growing bacillus. The bacterial strain is a halophilic fast-growing bacterium TBC005, and the preservation number of the halophilic fast-growing bacterium TBC005 is GDMCC No: 65236. The invention further discloses a preparation method of the halophilic fast-growing bacterium TBC005. The halophilic fast-growing bacillus TBC005 disclosed by the invention has an excellent degradation capability on chlorinated persistent organic pollutants, and the degradation rates of C10Cl6, TECP (Tetraethyl Cyclohexyl Phosphate) and TCPP (Transmission Control Protocol) can respectively reach 21.5 percent, 16.7 percent and 16.9 percent after the halophilic fast-growing bacillus TBC005 is cultured for 14 days under an anaerobic condition; after the strain is cultured for 35 days, the degradation rates of C10Cl6, TECP and TCPP can reach 50.7%, 59.2% and 61.3% respectively, and the strain can be applied to the fields of environmental pollution abatement and bioremediation of chlorinated persistent organic pollutants.

The invention provides a halophilic protein prediction method and system based on a hybrid deep learning architecture, and relates to the technical field of information analysis, and the method comprises the steps: obtaining a to-be-predicted protein sequence; extracting global semantic embedding features by using a protein language model; meanwhile, recoding the sequence based on a specific reduced amino acid alphabet, and counting reduced dipeptide frequencies in three interval modes to generate a local composition feature vector; and after the two types of features are spliced, inputting the spliced features into a mixed deep learning model formed by connecting a one-dimensional convolutional neural network and a lightweight Transform encoder in series for processing, and finally outputting a prediction result by a full connection layer. According to the method, by fusing the multi-scale sequence features and the hierarchical hybrid network architecture, the accuracy, generalization ability and calculation efficiency of halophilic protein recognition are remarkably improved, and an effective tool is provided for intelligent mining of large-scale extreme environment enzyme resources.

The invention discloses a photoaerobic organism complete mixing reactor for synthesizing purple membrane protein by enriching halophilic microorganisms. The photoaerobic organism complete mixing reactor comprises a biological reaction area and a bottom light management area, the biological reaction area is used for completing microbial cell proliferation, metabolism regulation and control and purple membrane protein synthesis, and providing a limit environment with constant temperature, controllable dissolved oxygen, uniform nutrition and sufficient illumination for halophilic microbial flora so as to realize accurate control of high-density culture and efficient induction of thalli; the bottom light management area is used for efficiently, uniformly and controllably providing light energy with the wavelength and intensity of 200-700nm to the biological reaction area, and the light energy is used as an environmental signal for inducing halophilic microorganisms to synthesize purple membrane protein. According to the reactor, the halophilic microbial flora for efficiently synthesizing the purple membrane protein can be obtained, an aerobic / illumination synergistic condition required by the purple membrane protein synthesis process is provided, sufficient salt and nutrient substances are given to the halophilic microbial flora, and a reactor operation mode for stably and efficiently synthesizing the purple membrane protein at low cost is realized.

The invention provides a screening method for rapidly identifying tetracoccus halophilus and application of the screening method, and belongs to the technical field of microorganisms. According to the application, specific screening and identification of the tetragenococcus halophilus are realized, genome extraction and on-machine sequencing of strains are not needed, the screening efficiency is improved, and the screening cost is reduced; based on specific gene amplification of biogenic amine generation key enzyme, screening of strains which do not generate biogenic amine is carried out, so that the screening efficiency is further improved, and efficient screening of industrial excellent-character tetracoccus halophilus strains is realized. By adopting the method disclosed by the invention, the identification of the tetracoccus halophilus and the screening of the tetracoccus halophilus which does not generate histamine and tyramine can be simultaneously carried out, high-throughput detection is realized, the labor cost and the time cost of gene extraction and sequencing are effectively reduced, and the problem that the performance and the quality of traditional fermented food are unstable is solved.

The present application relates to a kind of halophilic halophanum and its application.The strain name is halophilic halophanum ( Salinicola halophilus )A3, is preserved in China Microorganism Strain Preservation Management Committee (CGMCC), and the preservation address is China Beijing City Chaoyang District Beichen West Road No. 1 Yard No. 3 Institute of Microbiology, Chinese Academy of Sciences, and the preservation date is July 30, 2025, and the preservation number is CGMCC No.35460.The halophilic halophanum ( Salinicola halophilus ) provided by the present application can be used for the degradation of toluene in high-salt environment.

The application discloses a construction method of a halophilic genetically engineered bacterium with improved bacterioruberin yield, which comprises the following steps: PCR amplification of a gene; enzyme digestion of the PCR product and an expression vector; ligation of the enzyme-digested PCR product and the expression vector; transformation of the ligation product into an E. coli competent cell to obtain a recombinant plasmid; transformation of the recombinant plasmid into a halophilic archaea competent cell; verification and selection of the recombinant halophilic bacterium with successful transformation for induction and culture to make the halophilic bacterium synthesize bacterioruberin. The application constructs a halophilic genetically engineered bacterium expressing lycopene synthetase by using genetic engineering technology. Compared with an initial bacterium, the constructed halophilic genetically engineered bacterium can improve the yield of bacterioruberin, and the yield of bacterioruberin synthesized by the constructed halophilic genetically engineered bacterium is further improved when tryptophan is added for induction. Therefore, the constructed halophilic genetically engineered bacterium can obviously improve the synthesis amount of bacterioruberin, thereby providing an important bacterium for promoting the industrialization application of bacterioruberin.

The present invention relates to the technical field of microbial bacillus, and specifically provides a halophilic bacillus DY299 with the ability to inhibit biofilm and its application in the field of anti-MRSA microbial control agents, which is classified and named Bacillus halotolerans and has been deposited in the China Center for Type Culture Collection with a deposit number of CCTCC NO: M 20242371. The halophilic bacillus DY299 of the present invention has excellent ability to inhibit Staphylococcus aureus biofilm; the halophilic bacillus DY299 of the present invention has broad application prospects and huge economic value in the fields of microorganisms, biomedicine, etc.

The present invention relates to halophilic microorganisms. In particular, halophilic microorganisms that expresses citramalate synthase. A plasmid encoding an antitoxin-toxin system, and a halophilic microorganism comprising said plasmid are also described, and methods of producing halophilic organisms are also described herein.

The invention relates to the technical field of microbial bacillus, and particularly provides halophilic bacillus DY299 with biological membrane inhibition capability and application of the halophilic bacillus DY299 in the field of anti-MRSA microbial control agents, the halophilic bacillus DY299 is classified and named as Bacillus halotolans and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20242371. The halophilic bacillus DY299 disclosed by the invention has excellent capability of inhibiting a staphylococcus aureus biological membrane; the halophilic bacillus DY299 disclosed by the invention has a wide application prospect and a huge economic value in the fields of microorganisms, biological medicines and the like.

The invention relates to a method for preparing soybean paste. By introducing the step of high-temperature soy sauce mash inactivation in the fermentation process and then adding tetracoccus halophilus with the preservation number of CGMCC No. 26096, the brewing process of the soybean paste disclosed by the invention can obviously reduce the risk of white spots, and is particularly suitable for cooking noodles with soybean paste; the comprehensive sensory indexes of the fried bean paste noodles are improved from multiple dimensions of coloring speed, color effect, stability, sauce flavor and the like.

The invention relates to the technical field of microorganisms. Specifically, the invention relates to tetracoccus halophilus with the characteristic of low acetic acid yield, which can reduce the content of acetic acid, ensure the content of lactic acid in soy sauce mash, further promote the alcoholic fermentation of soy sauce yeast and contribute to greatly increasing the content of characteristic flavor substances in soy sauce. The invention also relates to a method for preparing soy sauce or sauce. The method comprises the step of adding the tetracoccus halophilus disclosed by the invention before soy sauce mash fermentation or in the soy sauce mash fermentation process.

Provided are a tetragenococcus halophilus with an inhibitory effect on bacillus cereus and a use thereof. The preservation number of the tetragenococcus halophilus is GDMCC 64945. The tetragenococcus halophilus is isolated from fermented bean curd, and colonies of the tetragenococcus halophilus are milky white, small and round on a solid medium; The tetragenococcus halophilus is Gram-positive, and when observed under a microscope, the morphology thereof is mostly diplococci or tetrads, with no chain-like formations. The strain is sensitive to eight antibiotics such as ampicillin, gentamicin and kanamycin, and has no drug resistance gene and virulence gene. A bacterial suspension of the strain has a good inhibitory effect on a bacillus cereus strain isolated from the fermented bean curd, and when the strain is applied to a fermented bean curd fermentation system, the growth of bacillus cereus in the system can be effectively inhibited, and the content of enterotoxin in the system is reduced.

The present invention relates to a microbiome composition of a fermentation culture supernatant of a halophilic Bacillus polyfermentus KMU01 strain having an anti-obesity effect, and a method for preparing the microbiome composition of the fermentation culture supernatant of the halophilic Bacillus polyfermentus KMU01 strain having the anti-obesity effect. The fermentation culture supernatant of the strain preserved with preservation number KCTC11751BP has been confirmed to inhibit fat production and accumulation and to reduce the content of blood cholesterol, and thus be used as a composition for preventing, treating or ameliorating obesity, or as a composition for reducing body fat (visceral fat) or blood cholesterol.

The present invention relates to the field of functional microorganism screening and application technology, and specifically provides a halophilic tetragenococcus (Tetragenococcus halophilus) with high protease activity and high production of bioactive peptides and its application. The halophilic tetragenococcus was screened from the traditional naturally fermented soybean paste in Northeast China, and the preservation number is CGMCC No.27586. The γ-glutamyl transferase activity of the strain is 7.361±0.003U / mL, the ability to scavenge DPPH free radicals reaches 57.32%, the ability to scavenge hydroxyl free radicals reaches 56.03%, and the protease activity is as high as 342.71±0.48U / mL, which are all higher than the standard strain ATCC 33315. The strain has high protease activity, high production of bioactive peptides, and has the potential for enhancing freshness while having good antioxidant capacity, and can be applied to the production of fermented functional foods.

The invention relates to the technical field of directional breeding and metabolism regulation of microorganisms in extreme environments, in particular to a halophilic microorganism mutant strain breeding method for efficiently fermenting seafood seasonings, which comprises the following steps: firstly, collecting samples from extreme environments such as deep-sea hydrothermal ports and the like, and putting the samples into an enrichment culture medium containing a growth promoting agent, special polysaccharide and marine microorganism source antibacterial peptide for culturing; separating single colonies by using a gradient dilution coating method; preparing a suspension from the single colony, sequentially carrying out plasma and chemical mutagenesis, and applying a low-intensity magnetic field; carrying out primary screening and secondary screening, and respectively screening by utilizing a fluorescent marker and a flavor enhancer; and finally, detecting the genetic stability of the passage strain through multiple technologies, and determining a final mutant strain. The breeding method has obvious advantages; the fermentation efficiency can be greatly improved, and the fermentation time is shortened; the genetic stability is guaranteed, and the production is controllable; pollution risk is reduced, food safety is guaranteed, and integration of marine organisms and food industry is promoted.

Provided are an engineered halophilic microorganism and a construction method and use therefor. By means of knockout or knockdown of one or more than two genes on a transposon and encoding a transposase, a stable halophilic microorganism is obtained, which has good growth condition, can stably synthesize polyhydroxy alkanoates, and can effectively avoid spontaneous mutation of the strain.