30 results about "Halorubrum halophilum" patented technology

The invention discloses a microbial agent containing halophilic denitrifying bacteria YL5-2 and application of the microbial agent. The microbial agent contains the halophilic denitrifying bacteria YL5-2, and the YL5-2 is a novel species of Halovibrio genus, and is preserved in the China General Microbiological Culture Collection Centre with a preservation number of CGMCC NO.16315 on August 20, 2018. The invention further discloses application of the microbial agent in high-salt wastewater treatment. The YL5-2 can be used for biological denitrification treatment of high-salt wastewater with salt content greater than 10%, and denitrification treatment cost of the wastewater is reduced. According to the invention, the microbial agent prepared from the halophilic denitrifying bacteria YL5-2 can be used for degradation, conversion and biological denitrification processes of pollutants under a high-salt condition, and the processes comprise high-salt wastewater treatment, polluted seawatergovernance, saline-alkali land repair, nitrogen nutrition consumption, inhibition on excessive multiplication of alga, water body purification, substrate improvement, and the like.

The invention aims at providing a halomonas sulfidaeris. The strain is named Halomonas sulfidaeris YZ7, and the strain is preserved in China Center for Type Culture Collection of Wuhan University, Wuhan, China in 6th, March, 2013, and the preservation number of the strain is CCTCC NO:M2013070. Halomonas sulfidaeris YZ7 disclosed by the invention has the capability of inhibiting algae blooms, and the strain is simple in nutrient requirement, so that the strain can be developed for an aquaculture microbial preparation and a waste water treatment agent, and the application prospect is wide.

The invention belongs to the field of bioengineering, and particularly relates to a preparation method of recombinant halophilic archaea protease. The method comprises the following steps: constructing a recombinant plasmid by taking a genome of Halostella sp. DL-M4 as a template and taking a pET28a plasmid as an expression vector; transforming escherichia coli cell by recombinant plasmid, inducing expression, centrifugally collecting thalli for cell resuspension and crushing, centrifuging again, taking a supernatant, adding beta-mercaptoethanol to obtain a mixed solution, enabling the mixed solution to pass through a nickel column, combining zymogen with a nickel medium for renaturation, and finally performing secondary impurity protein removal and target protein elution, performing ultrafiltration centrifugal concentration and performing gel filtration chromatography to obtain the recombinant halophilic archaea protease. The method provided by the invention has good universality, simpler purification steps and shorter production cycle, the obtained protease has high purity, and finally the halophilic archaea protease with specific enzyme activity of 1159.15 U/mg is obtained.

The invention belongs to the technical field of bioengineering, and particularly relates to halophilic archaea for degrading nitrite and an application of the halophilic archaea. According to the invention, a new species of Haloarcula is found, and the new species is named as Haloarcula sp. JB33. The strain is aerobic denitrifying bacteria, can grow within the salt concentration range of 5-25% andthe temperature range of 20-52 DEG C, and has good salt tolerance. The optimal growth temperature is 37 DEG C. When the salt concentration is 18% and the nitrite concentration is 100mg/kg-500mg/kg, the strain still normally grows and effectively degrades nitrite, and the optimal degradation rate reaches 98.59% after the strain is degraded for 96 hours. Nitrite degradation effect is obvious. The strain can be used for degrading the nitrite in foods. The halophilic archaea is particularly applied to degradation of the nitrite in high-salt pickled foods such as pickled vegetables and hams.

The invention discloses a preparation method of fermented bean curd. The preparation method comprises the following steps of: S1, preparing soybean milk: soaking soybeans, grinding the soybeans into thick liquid, filtering the thick liquid, boiling the thick liquid, and sieving the thick liquid to obtain bean dregs and soybean milk; S2, preparing a blank: curdling the soybean milk with beneficial bacterium acid water with the acidity of 0.7-1.0 g/100ml, squeezing the curdled soybean milk, and performing medium emulsification to obtain a white blank; S3, spraying seeds: uniformly mixing 5-15% of lactococcus lactis or tetragonococcus halophilus with 85-95% of mucor strains to obtain a beneficial bacterium mixed bacterium solution, inoculating the beneficial bacterium mixed bacterium solution to the white blank, and performing fermentation to obtain a blank after the fermentation is mature; S4, performing pickling: pickling the blank to obtain a salted blank; and S5, performing after fermentation: uniformly mixing 5-10% of tetragonococcus halophilus or lactococcus lactis with 90-95% of semi-finished product juice to obtain beneficial bacterium juice, filling the salted blank with the beneficial bacterium juice to form a semi-finished product, and performing after fermentation. According to the preparation method disclosed by the invention, the quality problems of white points, gas production, juice leakage and the like are controlled in a targeted manner, and fermented bean curd products with stable quality are produced.

The invention discloses a biological flocculant, which belongs to the field of environment-friendly microorganisms and is prepared from the following raw materials in parts by weight: 70 to 90 parts of bacillus halophilus LH-B.0015 fermentation liquor, 5 to 15 parts of bentonite, 1 to 5 parts of diatomite, 1 to 5 parts of ferrous sulfate, 1 to 5 parts of magnesium carbonate and 2 to 5 parts of calcium chloride. The invention also discloses a preparation method and application of the biological flocculant. The salt tolerance range of the halophilic bacillus amyloliquefaciens LH-B.0015 contained in the biological flocculating agent is 1%-30%, and the flocculating agent can be synthesized within the salinity range of 1%-25%. The strain is used for biological flocculation in a high-salt environment, can effectively solve the flocculation problem in the high-salt environment, reduces the treatment cost, and has a good application prospect.

The invention relates to the technical field of functional microorganism screening and application, and particularly provides a strain of novel Tetragenococcus halophilus and application of the novel Tetragenococcus halophilus. The Tetragenococcus halophilus is screened from traditional naturally-fermented soybean paste in Northeast, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 20, 2021, wherein the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 23165. The strain can greatly improve the yield of umami peptide, is beneficial to reducing the production cost of the umami peptide, and promotes the wide application of the umami peptide in the food field.

The invention relates to the field of sewage treatment, and discloses a halophilic brevibacterium linum strain capable of efficiently degrading ammonia nitrogen and application of same, wherein the brevibacterium linum is named as B1, has been preserved in China General Microbiological Culture Collection Center (CGMCC) on July 2, 2019, the preservation number of the brevibacterium linum is CGMCC NO.18298, and the microorganism classification of the brevibacterium linum is named as Brevibacterium lines. The brevibacterium linum B1 has strong ammonia nitrogen degradation capability, can tolerate a high-salt environment, can grow and maintain high ammonia nitrogen removal efficiency under a salinity condition of 1%-10%, can be applied to high-salt sewage ammonia nitrogen degradation treatment, and has the advantage of short treatment time.

The invention provides a method for producing alginate lyase through co-fermentation of halophilic lactic acid bacteria and marine bacteria, which comprises the following steps: culturing a marine bacteria XS1412C strain in advance, inoculating the XS1412C strain into a fermentation culture solution to prepare an XS1412C strain fermentation culture solution, and culturing halophilic lactic acid bacteria in advance to produce alginate lyase. The method comprises the following steps: inoculating halophilic lactic acid bacteria into an XS1412C strain fermentation culture solution for symbiotic fermentation culture to obtain a symbiotic fermentation culture solution, and finally centrifuging the symbiotic fermentation culture solution to obtain an enzyme solution containing alginate lyase. Halophilic lactic acid bacteria capable of generating beneficial substances through fermentation, promoting growth of strains and improving enzyme production capacity of the strains and XS1412C strains capable of secreting alginate lyase are subjected to symbiotic fermentation culture, secretion of alginate lyase can be promoted, enzyme quality can be guaranteed, enzyme activity can be effectively improved, the fermentation process can be simplified, fermentation time can be shortened, and extraction cost can be reduced. The invention belongs to the technical field of marine organisms.

The invention discloses halophilic salinicola sp.W-Y12 and application thereof. The classification name of the halophilic salinicola sp.W-Y12 is Salinicola sp. The strain was preserved in China General Microbiological Culture Collection Center on November 13th, 2018 with a preservation number of CGMCC No.16742. An experiment proves that the halophilic salinicola sp.W-Y12 disclosed by the inventioncan emulsify crude oil and can be used for microbe oil recovery to improve the oil recovery rate; in addition, the halophilic salinicola sp.W-Y12 also can be used for treating and repairing of petroleum pollution and has a good application prospect.

The invention discloses a method for evolution and domestication of halophilic microalgae, and belongs to the field of biomass resource development and utilization. The method for evolution and domestication of the halophilic microalgae comprises the steps that the halophilic microalgae is separated into salt algae colonies, and then activation is carried out; a multi-step adaptive evolution method is used for reducing cell loss of the halophilic microalgae caused by sudden change in osmotic pressure, and repeated domestication is carried out on the microalgae by a LED photobioreactor. According to the method for evolution and domestication of the halophilic microalgae, the salinity setting is adjusted and controlled, the environmental pressure is changed reasonably, and the adaptive evolution of the halophilic microalgae is achieved, so that the lutein production under seawater-like salinity culture conditions is improved. The method for evolution and domestication of the halophilic microalgae has the characteristics of environmental friendliness and is beneficial to the utilization of marine resources and environmental protection, adaptive evolution and domestication of the halophilic microalgae driven by environment stress is carried out by gradually reducing the salinity, so that the level of lutein produced by salt algae is significantly improved, and the good industrial prospect is achieved.

The invention relates to the field of gene engineering, in particular to xylanase Scxyn5 and a coding gene and application thereof. The amino acid sequence of the gene is as shown in SEQ ID NO: 1. The invention provides xylanase Scxyn5 of Schizophyllum commune, the xylanase Scxyn5 is an extremely salt-tolerant/halophilic xylanase, and a high-salt-concentration catalytic system can improve the hydrolytic activity of the xylanase to corn viscose, so that the corn viscose can be degraded by the xylanase Scxyn5, and the corn viscose can be degraded by the xylanase Scxyn5, so that the corn viscose can be degraded by the xylanase Scxyn5. Arabinoxylan with different component force distribution characteristics can be prepared by applying xylanase single enzyme to hydrolyze corn viscose, and a basis is provided for industrially preparing functional arabinoxylan with specific molecular weight by taking corn bran as a raw material.