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Probe, kit and detection method for rapidly detecting bombyx mori nuclear polyhedrosis virus

A technology of nuclear polyhedron and detection method, which is applied in the field of probes for rapid detection of silkworm nuclear polyhedrosis virus, can solve the problems of cumbersome fluorescent quantitative PCR technology detection and high inspection facility conditions, and achieve the reduction of reagent mixing and sampling steps, Detect fast, time-saving effects

Active Publication Date: 2022-03-25
广西壮族自治区蚕业技术推广站
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, the commonly used methods to detect whether silkworms have karyotype polyhedrosis are PCR nucleic acid amplification technology and fluorescent quantitative PCR technology, but PCR nucleic acid amplification technology has the problem of high inspection facilities, and fluorescent quantitative PCR technology has the problem of cumbersome detection

Method used

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  • Probe, kit and detection method for rapidly detecting bombyx mori nuclear polyhedrosis virus
  • Probe, kit and detection method for rapidly detecting bombyx mori nuclear polyhedrosis virus
  • Probe, kit and detection method for rapidly detecting bombyx mori nuclear polyhedrosis virus

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Embodiment Construction

[0040] The present invention will be further described in detail below in conjunction with the embodiments, so that those skilled in the art can implement it with reference to the description.

[0041]

[0042] 1. Synthesis of probes and primers

[0043] Since the length of the probe sequence needs to be between 40-50bp, and the quenching group and the fluorescent group are respectively connected to a pair of thymine nucleotides, the pair of oligo-Ts are separated by 1-5 bases , one of the heterozygous 1-5 bases is replaced by tetrahydrofuran, pre-designed in the full-length 1755bp of the BmNPV ie-1 gene, the probe starts from 1469-1508bp, a total of 40bp, quencher group and fluorescent group The groups are respectively connected at positions 1493 and 1497, and the 149th thymine is replaced by tetrahydrofuran to form the desired detection probe (BmNPV-probe). The nucleotide sequence of BmNPV-probe is: TCAAAAACGAAGAGCGGTTGACTA / i6FAMdT / [THF]GC / iBHQ1dT / AAGAAAAACGA, based on t...

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Abstract

The invention discloses a probe for rapidly detecting a bombyx mori nuclear polyhedrosis virus. The bombyx mori nuclear polyhedrosis virus can be rapidly detected through an RAA technology. The invention further discloses a kit for rapidly detecting the bombyx mori nuclear polyhedrosis virus, and the kit is rapid in detection and high in sensitivity. The invention further discloses a detection method of the kit for rapidly detecting the bombyx mori nuclear polyhedrosis virus, the steps of reagent mixing and sample adding are reduced, operation is convenient, and time is saved.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically, to a probe, a kit and a detection method for rapidly detecting the silkworm nuclear polyhedrosis virus. Background technique [0002] Bombyx mori karyopolyhedrosis is the oldest viral silkworm disease, which occurs in all ages and developmental stages of silkworms. At present, the commonly used methods for detecting whether silkworms have karyotype polyhedrosis are PCR nucleic acid amplification technology and fluorescent quantitative PCR technology, but PCR nucleic acid amplification technology has the problem of high inspection facilities, and fluorescent quantitative PCR technology has the problem of cumbersome detection. Contents of the invention [0003] It is an object of the present invention to solve at least the above-mentioned problems and to provide at least the advantages which will be described later. [0004] Another object of the present invention is to provide a ...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/6844C12N15/11C12R1/93
CPCC12Q1/701C12Q1/6844C12Q2521/507C12Q2563/107Y02A50/30
Inventor 唐亮潘志新唐明艳蒋满贵董桂清王霞黄深惠陈小青赵烨芸
Owner 广西壮族自治区蚕业技术推广站
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