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High-yield cellobiase strain as well as construction method and application thereof

A cellobiase and strain technology, which is applied in the field of bioengineering to achieve the effects of improving cellulase system and improving cellobiase activity

Pending Publication Date: 2022-04-01
ZHONGRONG TECH CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, how to obtain a strain with high cellobiase production is a difficult problem

Method used

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  • High-yield cellobiase strain as well as construction method and application thereof
  • High-yield cellobiase strain as well as construction method and application thereof
  • High-yield cellobiase strain as well as construction method and application thereof

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Embodiment 1

[0053] The present embodiment provides a method for constructing a high-yield cellobiase strain, comprising the following steps:

[0054] 1. The promoter PpgdA gene and the cellobiase coding gene CB constitute an expression element integrated into the pGPD-hph vector;

[0055] 2. Integrating the PpgdA gene, CB gene and hph hygromycin gene combination elements into the pPK2 vector;

[0056] 3. Using Agrobacterium tumefaciens to mediate the transformation of Trichoderma reesei and obtain a Trichoderma reesei engineering strain with high cellobiase production.

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Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a high-yield cellobiase strain as well as a construction method and application thereof. A promoter PpgdA gene and a cellobiase coding gene CB form an expression element, and the expression element is integrated on a PGPD-hph vector; the method comprises the following steps: integrating a promoter PpgdA gene, an hph hygromycin gene and a CB gene combined element onto a pPK2 vector; agrobacterium tumefaciens is used for mediating conversion of trichoderma reesei to obtain a trichoderma reesei engineering strain with high yield of cellobiase, namely the strain with high yield of cellobiase. Compared with the prior art, the method has the advantages that the cellobiase gene is integrated into the trichoderma reesei, so that the activity of the cellobiase in the trichoderma reesei is greatly improved, the cellulase system of the trichoderma reesei is improved, and the enzymatic saccharification of renewable resources is greatly developed.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a strain with high cellobiase production and its construction method and application. Background technique [0002] Cellulose, hemicellulose, and lignin account for 30-40%, 15-30%, and 10-20% of the fibrous raw materials, respectively. As the main polysaccharide product of plant photosynthesis, cellulose is the most abundant renewable natural resource on earth. It is estimated that the energy contained in cellulose on the earth is approximately equivalent to the energy contained in 640 billion tons of petroleum, and the renewability of cellulose is incomparable to mineral energy such as petroleum. As an industry with great development potential, cellulosic ethanol has attracted more and more attention and attention at home and abroad. The production process of cellulosic ethanol mainly includes raw material pretreatment, hydrolysis, fermentation and distillat...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/80C12N15/65C12N15/90C12N15/56C12N1/21C12N1/15C12P19/14C12P19/02C12R1/885
Inventor 杨何宝郭世奇吴超勇代淑梅
Owner ZHONGRONG TECH CORP LTD
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