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Dactylis glomerata vernalization gene DgPAPS4 and application thereof

A technology of gene and Dactylis, which is applied in the field of cloning the primer set of the gene from plant DNA, can solve the problems of large variation in Dracula flowering period, lack of Dracula species, and limited application, so as to reduce the breeding workload and improve breeding efficiency , the effect of shortening the breeding period

Inactive Publication Date: 2022-04-05
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the flowering period of Dactylis chinensis varies greatly, and there is a lack of varieties of Dactylis chinensis suitable for flowering period in actual production, which seriously limits its application in production
Years of research on the flowering period of Dactylis chinensis has been conducted with little effect

Method used

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  • Dactylis glomerata vernalization gene DgPAPS4 and application thereof
  • Dactylis glomerata vernalization gene DgPAPS4 and application thereof
  • Dactylis glomerata vernalization gene DgPAPS4 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] This example describes a vernalization gene DgPAPS4 that can be expressed in Dactylis chinensis to regulate the flowering time of Dactylis chinensis. The vernalization gene DgPAPS4 comprises a nucleotide sequence selected from the following group:

[0036] A. The nucleotide sequence shown in Seq ID NO.1 of the sequence table.

[0037] B. Nucleotide sequence complementary to the sequence described in A. Or a nucleotide sequence with more than 70% homology, such as a nucleotide sequence with more than 80% homology, a nucleotide sequence with more than 90% homology, or a nucleotide sequence with more than 95% homology .

[0038] C. The nucleotide sequence encoding the amino acid sequence shown in Seq ID NO.2 of the sequence table.

Embodiment 2

[0040] This example describes a protein whose amino acid sequence is shown in Seq ID NO.2. The sequence is encoded by the vernalization gene DgPAPS4(A) in Example 1.

Embodiment 3

[0042] This example describes the primer pair for cloning the vernalization gene PAPS4 of Dactylis chinensis. The base sequences of the primer pair are respectively the upstream primer F shown in the sequence table Seq ID NO.3: 5'-atggtaccatgacgacctctaataagccgatat-3', the sequence table Downstream primer R shown in Seq ID NO.4: 5'-atcgctagcctacaaactaatgcatggccatttc-3'. Using the pair of primers, the nucleotide sequence shown in the sequence table Seq ID NO.1 can be extracted from the DNA of Dactylis chinensis.

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Abstract

The invention discloses a dactylis glomerata vernalization gene DgPAPS4 and application thereof, the vernalization gene DgPAPS4 is expressed in a plant to adjust the flowering time of the plant, the gene DgPAPS4 comprises a nucleotide sequence selected from the following groups: A, a nucleotide sequence shown in a sequence table Seq ID NO.1; b, a nucleotide sequence complementary to the sequence of A, or a nucleotide sequence with more than 70% of homology; c, a nucleotide sequence of an amino acid sequence as shown in Seq ID NO.2 in a coding sequence table; the invention also provides an expression vector containing the vernalization gene DgPAPS4, a host cell containing the overexpression vector, and an application of the vernalization gene DgPAPS4. The vernalization gene DgPAPS4 provided by the invention can promote flowering of plants with vernalization by regulating and controlling expression of vernalization pathway related genes, facilitates cultivation of plant varieties with vernalization in different ripening stages, reduces breeding workload, reduces breeding scale, shortens breeding age limit, improves breeding efficiency, and has a wide application prospect. And the breeding of plant varieties with vernalization is accelerated.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and relates to a gene that affects plant vernalization and flowering, a protein encoded by the gene, an expression vector and a host cell containing the gene, and a primer set for cloning the gene from plant DNA , also relates to the use of the gene. Background technique [0002] Dactylis glomerata L., also known as Orchard grass (Orchardgrass), belongs to Gramineae (Gramineae) Pooideae subfamily (Pooideae) Dactylis (Dactylis), is a perennial cool-season bushes widely cultivated worldwide. grass. Dactylis has the characteristics of fast growth, high biomass, high sugar content, strong shade tolerance and wide adaptability. As the top four perennial grasses in economic value, Dactylis is important for the production of meat and dairy products of herbivores in temperate regions of the world. In addition to being an excellent forage grass, Dactylis is also one of the important mixed-sowin...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/84A01H5/02A01H6/46
CPCC07K14/415C12N15/827Y02A40/146
Inventor 张新全杨忠富黄琳凯聂刚冯光燕李丹丹汪霞周冀琼
Owner SICHUAN AGRI UNIV
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