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Glutamic oxalacetic transaminase determination reagent, preparation method of reagent ball and determination chip

A technology for aspartate aminotransferase and reagents, which is applied in biochemical equipment and methods, determination/inspection of microorganisms, etc., can solve problems such as insufficient detection accuracy and limited reference significance, and achieve the effects of improving stability and accuracy.

Pending Publication Date: 2022-04-08
GENRUI BIOTECH INC
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Problems solved by technology

[0003] However, most of the reagents currently used clinically for the determination of alanine aminotransferase are not accurate enough, and have relatively limited reference significance for clinical use.

Method used

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  • Glutamic oxalacetic transaminase determination reagent, preparation method of reagent ball and determination chip
  • Glutamic oxalacetic transaminase determination reagent, preparation method of reagent ball and determination chip
  • Glutamic oxalacetic transaminase determination reagent, preparation method of reagent ball and determination chip

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preparation example Construction

[0048] The embodiment of the present application also provides a preparation method of aspartate aminotransferase assay reagent ball, please refer to figure 1 , including the following steps:

[0049] S10: adding the buffer solution to a preset amount of water;

[0050]Specifically, a certain milliliter of distilled water can be added to a beaker, and then a certain amount of buffer components can be weighed, added to the distilled water, and stirred until completely dissolved.

[0051] S20: After the buffer solution is completely dissolved in water, add L-aspartic acid and α-ketoglutaric acid in sequence, and adjust the pH value to the first preset pH value to obtain the first solution;

[0052] Add L-aspartic acid and α-ketoglutaric acid in sequence and make them dissolve completely, and adjust the pH value of the solution after the dissolution is complete.

[0053] In some embodiments, the first preset pH value is 7.5-7.7.

[0054] S30: adding an excipient and a stabiliz...

Embodiment 1

[0078] The above-mentioned aspartate aminotransferase assay reagent can be the following components: tris buffer solution 50mmol / L, L-aspartic acid 800mmol / L, α-ketoglutarate 100mmol / L, trehalose 10g / L, bovine Serum albumin 5g / L, Triton X-1000.1g / L, reduced nicotinamide adenine dinucleotide 0.5mmol / L, malate dehydrogenase 5U / L, D-lactate dehydrogenase 1U / L , the pH value of the aspartate aminotransferase assay reagent is 7.6.

Embodiment 2

[0080] The above-mentioned aspartate aminotransferase assay reagent can be the following components: tris buffer solution 160mmol / L, L-aspartic acid 710mmol / L, α-ketoglutarate 80mmol / L, trehalose 4g / L, bovine Serum albumin 1.2g / L, Triton X-100 2.5g / L, reduced nicotinamide adenine dinucleotide 0.3mmol / L, malate dehydrogenase 3U / L, D-lactate dehydrogenase 6U / L, the pH value of the aspartate aminotransferase assay reagent is at 7.9.

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Abstract

The embodiment of the invention relates to the technical field of detection reagents, in particular to a glutamic oxalacetic transaminase determination reagent, a preparation method of a reagent ball and a determination chip. The glutamic oxalacetic transaminase determination reagent provided by the embodiment of the invention comprises a buffer solution, L-asparaginic acid, alpha-ketoglutaric acid, an excipient, a stabilizer, a surfactant, reduced nicotinamide adenine dinucleotide, malate dehydrogenase and D-lactic dehydrogenase. The glutamic oxalacetic transaminase determination reagent contains an effective stabilizer component, and the stability of enzyme and reduced nicotinamide adenine dinucleotide in the determination reagent is improved, so that the stability is better when the concentration of glutamic oxalacetic transaminase is detected, and the accuracy of a determination result is improved.

Description

technical field [0001] The embodiment of the present application relates to the technical field of detection reagents, in particular to a reagent for assaying aspartate aminotransferase, a method for preparing reagent balls, and an assay chip. Background technique [0002] Aspartate aminotransferase is widely distributed in various organs and tissues, mainly concentrated in the liver, heart and kidney. Aspartate aminotransferase has the highest content in cardiomyocytes. When cardiomyocytes are damaged, a large amount of enzymes are released into the blood, increasing the serum content. It is usually used as an auxiliary examination for myocardial infarction and myocarditis in clinical practice. [0003] However, most of the reagents currently used clinically for the determination of alanine aminotransferase are not high enough in detection accuracy, and have relatively limited reference significance for clinical use. Contents of the invention [0004] The embodiment of t...

Claims

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Application Information

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IPC IPC(8): C12Q1/52
Inventor 牟健周慧欣陈明
Owner GENRUI BIOTECH INC
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