40 results about "Malate quinone oxidoreductase" patented technology

The invention relates to mutants and alleles of the coryneform bacterium mqo gene which encodes malate quinone oxidoreductases which contain any amino acid apart from L-serine at position 111, or a comparable position, in the amino acid sequence, and to processes for fermentatively preparing amino acids, preferably L-lysine, L-tryptophan and L-proline, using bacteria which comprise these alleles.

The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.

Provided are an engineered strain for synthesizing a dibasic organic acid and preparation and application of same. The engineered strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and / or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid, as compared with the origin strain of the engineered strain, the producing capability for producing the dibasic organic acid is improved. The dibasic organic acid comprises malic acid, succinic acid, fumaric acid, oxaloacetic acid, glutaric acid, and adipic acid; the expression product of the positive regulator gene comprises aspartate aminotransferase, glutamic acid-aspartate transporter, C4-dicarboxylic acid transporter, pyruvate carboxylase and malate dehydrogenase, glucose transporter; the expression product of the negative regulatory gene comprises succinyl-CoA synthase, and malic acid-alpha ketoglutarate transporter, and the original strain comprises myceliophthora thermophila, thielavia terrestris, aspergillus, and rhizopus.

The invention provides malic dehydrogenase PbMDH derived from Pseudomonas beteli, as well as a coded gene and an application of the malic dehydrogenase. Cloned plasmid with the malic dehydrogenase PbMDH nucleotide sequence can be transferred into engineering bacteria by transduction, transformation and conjugal transfer, the malic dehydrogenase PbMDH can be efficiently expressed by adjusting expression of the coded gene, and an effective way is provided for production of the malic dehydrogenase. The marine-derived malic dehydrogenase is resistant to salt and high temperature and has importantindustrial application prospects.

The invention relates to the field of in vitro diagnosis, in particular to a low-temperature and high-efficiency carbon dioxide kit using a cycle enzyme method. The kit is composed of NADH analog cyclic regeneration system and carbon dioxide determination reagent. The NADH analog cyclic regeneration system consists of 6-phosphate-fructose dehydrogenase 5-50U / L, hexokinase 50-500U / L, fructose 1- 5g / L, adenosine triphosphate disodium salt 0.5-2.5g / L, carbon dioxide determination reagent consists of Tris-hydrochloric acid 0.1mol / L (pH=8), stabilizer 1g / L, oxaloacetic acid 0.3g / L, composed of phosphoenolpyruvate carboxylase 0.1g / L, potassium phosphoenolpyruvate 0.6g / L, low temperature malate dehydrogenase 0.05g / L, Triton-100 1g / L. The carbon dioxide measuring kit of the present invention can be used at a lower temperature to detect the carbon dioxide concentration in a high-salt sample.

The invention discloses a heat shock protein MaltHSP20‑11 of Monochamus alternatus and its coding gene and application, belonging to the field of heat shock proteins. The full length of the heat shock protein HSP20 gene of Monochamus alternatus provided by the invention is 531 bp, encoding 176 amino acids. The expression characteristics of this gene under heat stress conditions were obtained by fluorescence quantitative method, and high temperature can obviously induce the up-regulation of this gene, indicating that this protein plays an important role in the resistance of Monochamus alternata larvae to high temperature stress. Finally, a prokaryotic expression vector was constructed, and the purified heat shock protein MaltHSP20‑11 of Monochamus alternata was successfully obtained through in vitro expression. to you + The heat shock protein MaltHSP20 of Monochamus alternatus purified by resin column was verified for molecular chaperone activity, which showed that the protein could effectively protect the heat coagulation of malate dehydrogenase. Therefore, this application has extremely high application value, and also provides a molecular basis for the research on the heat resistance of Monochamus alternatus.

The invention provides a malate dehydrogenase related to high light efficiency and stress resistance of wheat, a gene encoding the enzyme and a plant expression vector containing the gene. The invention also provides a method for cultivating transgenic plants with high light efficiency and stress resistance by using the gene of the invention.