A kind of malate dehydrogenase pbmdh and its coding sequence and application

A technology of malate dehydrogenase and sequence, which is applied in the field of genetic engineering and enzyme engineering, and can solve the problems of uncommon mining of malate dehydrogenase
CN109337879BActive Publication Date: 2021-01-26XIAMEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
XIAMEN UNIV
Publication Date
2021-01-26

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Abstract

The invention provides malic dehydrogenase PbMDH derived from Pseudomonas beteli, as well as a coded gene and an application of the malic dehydrogenase. Cloned plasmid with the malic dehydrogenase PbMDH nucleotide sequence can be transferred into engineering bacteria by transduction, transformation and conjugal transfer, the malic dehydrogenase PbMDH can be efficiently expressed by adjusting expression of the coded gene, and an effective way is provided for production of the malic dehydrogenase. The marine-derived malic dehydrogenase is resistant to salt and high temperature and has importantindustrial application prospects.
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Description

technical field

[0001] The present invention relates to the technical fields of genetic engineering and enzyme engineering, and relates to a malate dehydrogenase PbMDH and its coding sequence and application, in particular to a malate dehydrogenase of marine-sourced Pseudomonas beteli PbMDH and its coding genes and applications. Background technique

[0002] Malate dehydrogenase (EC: 1.1.1.37) widely exists in animals, plants and various organisms, and is highly conserved. It plays an important role in metabolism such as circulation and amino acid synthesis. There are different approaches to the classification of malate dehydrogenase. Classified according to its distribution in eukaryotic cells, malate dehydrogenase can be mainly divided into cytoplasmic malate dehydrogenase, mitochondrial malate dehydrogenase and chloroplast malate dehydrogenase, which exist in the cytoplasm, mitochondria and In chloroplasts, in addition, subcellular localization, malate dehydrogenase wa...

Claims

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