Production method for sucrose phosphorylase and application of production method

A sucrose phosphorylase and a technology for producing sucrose, applied in the biological field, can solve the problems of low specific enzyme activity of sucrose phosphorylase, difficulty in meeting industrial applications, poor soluble expression effect, etc., and achieve high yield
CN110656077AActive Publication Date: 2020-01-07JIANGNAN UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2020-01-07

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Abstract

The invention discloses a production method for sucrose phosphorylase and an application of the production method, and belongs to the field of biotechnology. The invention provides the production method for sucrose phosphorylase. The method produces the sucrose phosphorylase with high yield and high specific enzyme activity. By the method, recombinant escherichia coli is expressed in a fermentation medium containing L-arabinose, tetracycline and IPTG for 12 hours by induction, allowing enzyme activity of the sucrose phosphorylase in a supernatant of cell disruption liquid up to 32.43U / mL, andspecific enzyme activity of the produced sucrose phosphorylase up to 18.5 U / mg.
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Description

technical field

[0001] The invention relates to a method for producing sucrose phosphorylase and its application, belonging to the field of biotechnology. Background technique

[0002] Sucrose phosphorylase (SPase, EC 2.4.1.7) belongs to the GH13 family and mainly exists in Bifidobacterium longum, Leuconostoc mesenteroides and Pseudomonas saccharophila.

[0003] Sucrose phosphorylase is active on various substrates and can catalyze various substrates to generate corresponding products. Therefore, it has a wide range of applications in industries such as food, cosmetics and medicine. At present, the application of sucrose phosphorylase mainly includes three aspects: (1) use sucrose as a donor, and use rhamnose, xylose, fructose and galactose as acceptors to catalyze the corresponding oligosaccharides. Oligosaccharides will have one more glucose group, which can be used as an additive in food and medicine; (2) catalyze certain unstable substances to synthesize their stable de...

Claims

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