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Human sperm morphological staining reagent and staining method

A dyeing method and dyeing reagent technology, applied in the field of human sperm detection, can solve problems such as complicated operation, and achieve the effect of simple operation and short time-consuming.

Pending Publication Date: 2022-04-08
SHANGHAI KEHUADIAGNOSITIC MEDICAL PRODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0015] 1. This product needs to be used in conjunction with an optical microscope (40-100X magnification).

[0016] 2. Shake the bottle before use.

[0017] 3. Take the same amount of sperm staining solution and sperm sample, 50 μL of SVS stain and 50 μL of sperm sample, and mix them evenly in Eppendorf test tubes.

[0018] 4. Leave at room temperature for 15-30 seconds.

[0019] 5. Prepare slides.

[0020] 6. Use a pipette to place a 20 μL droplet on the marked microscope slide and draw a line in the middle of the slide with the droplet.

[0021] 7. Cover this slide with another microscope slide. When the droplet is evenly dispersed in the middle of the two slides, pull the two slides apart horizontally, and there will be two prepared slides. piece.

[0022] 8. Air dry both slides and test. It is also possible to use DPX or other equivalent mounting medium and coverslips, stack the slides and store them for subsequent testing.

[0023] 9. After covering with oil droplet...

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Abstract

The invention relates to the field of human sperm detection, in particular to a human sperm morphological staining reagent and a staining method.The human sperm staining solution SVS comprises a 0.1-1 M sodium chloride solution, a 20-100 mM eosin solution, a 100-200 g / L nigrosine solution, a 0.01-1% formaldehyde solution and a 1 M sodium hydroxide solution, and the pH value is adjusted to be 7.3-9.5 through a hydrochloric acid solution. The method is a one-step method and is simple and efficient, all dyeing requirements can be met by only one bottle of reagent, dyeing can be completed only in 30-60 seconds, the sperm morphology of the human sperm dyeing agent is observed under a microscope after air drying, the operation is simple, and the consumed time is short.

Description

technical field [0001] The invention relates to the field of human sperm detection, in particular to a human sperm morphology staining reagent and a staining method. Background technique [0002] The invention is applied in the medical industry, suitable for the current assisted reproductive technology, and can be used for dyeing sperm, and then performing morphological detection of sperm. Morphological detection of sperm is an important indicator for evaluating sperm function and male fertility. The development of it has received increasing clinical attention. [0003] Existing sperm staining methods, such as modified Papanicolaou staining method and Papanicolaou staining method, have complicated formulas and long operation time; Diff-Quik method, which was originally a conventional rapid staining method for leukocyte classification detection, is easy and fast to operate , but its raw materials are expensive and difficult to purchase. [0004] The Chinese invention patent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N1/28
Inventor 张献新安淑英吴迪朱德新
Owner SHANGHAI KEHUADIAGNOSITIC MEDICAL PRODS