In-vitro preparation method of fertile Chinese rose pollen
A technology for rose and pollen, applied in the fields of botanical equipment and methods, gardening methods, gardening tools/equipment, etc., can solve the problems of affecting application, lack of flowering, and complicated operation.
Active Publication Date: 2022-04-12
SHANGHAI CHENSHAN BOTANICAL GARDEN
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Problems solved by technology
In nature, the production of pollen is limited by seasons, and the parents have problems such as not meeting the flowering period and short pollen lifespan (Shang Xiaoqian. Research on cryopreservation of peony pollen [D]. Beijing Forestry University, 2005.), which seriously affects its haplotype. Applications in sports breeding and hybrid breeding
Cryogenic preservation is a new technology developed in recent decades. It is considered to be the only way for long-term preservation of pollen, but there are problems such as cumbersome operation and reduced pollen viability (Li Zedi. Evaluation of the effect of ultra-low temperature preservation of garden plant pollen and its physiological mechanism Research [D]. Beijing Forestry University, 2019.), the test-tube flowering of roses can obtain breeding materials all year round while breaking the restrictions of natural conditions, physiological isolation and parental flowering period.
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[0036] The specific implementation manner of the present invention is described in detail below, but it does not constitute a limitation to the present invention.
[0037] 1. Preparation of explant material
[0038] Take the rose branches of semi-lignified fairy mirror species that grow robustly in spring, wash them with running water for 0.5 hours, wash them with 5‰ potassium permanganate shock for 5 minutes, place them on a clean bench, treat them with 75% ethanol for 30 seconds, and culture them with 50% plant tissue Antibacterial protective agent (Plant Preservative Mixture, PPM) soaked 5-15min (Table 1), inoculated to MS medium supplemented with 3mg / L 6-BA, 0.3mg / LNAA, 0.1-0.5%PPM (V / V). Cultivate for 2 weeks, and count the material contamination and growth status of each treatment. The results showed that with the prolongation of PPM treatment time, the cleaning rate gradually increased, and the cleaning rate of PPM15 min treatment reached 98%, and the survival rate gra...
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According to the method, a semi-lignified Chinese rose branch is used as an explant, the Chinese rose is induced to bloom in a bottle through the steps of high-frequency synchronous adventitious bud induction, adventitious bud elongation, Chinese rose reproductive transformation, fertile pollen induction culture and the like, functional pollen is generated, and the in-vitro preparation method of the fertile Chinese rose pollen is established. The method provided by the invention can meet the growth conditions of the Chinese roses in nature, so that the Chinese roses grow without space-time factors, materials are provided for test tube hybridization, and the breeding cycle is accelerated; meanwhile, the produced fertile pollen can be used as a starting material for haploid culture, and an excellent material is provided for Chinese rose breeding.
Description
technical field [0001] The invention belongs to the field of plant tissue culture and breeding, in particular to a method for preparing fertile rose pollen in vitro. Background technique [0002] Rose (Rosa chinensis Jacq.), a plant belonging to the Rosaceae (Rosaceae Juss.) genus Rosa (Rosa L.), known as the "Queen of Flowers", one of China's top ten famous flowers, one of the world's four major cut flowers (Chinese rose Development Report (2nd Edition) [J]. Agricultural Science and Technology and Information (Modern Garden), 2014, 11(05): 44-47+1-43.), has high ornamental value and economic value (Ma Xuefan. Rose Research on hybrid breeding [J]. Farm Staff, 2019(17): 18.). With the development of the times, the breeding direction of roses mainly focuses on the external morphology and the ornamental characteristics of varieties, such as color, plant type and flower fragrance (Hu Qingpo, Li Xuefeng, Wu Qiong, Li Xinwei, Meng Yuanyuan, Liu Wei. Modern Rose Breeding Research[...
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IPC IPC(8): A01H4/00
Inventor 徐子岩朱木兰周慧晶郑珂瑗
Owner SHANGHAI CHENSHAN BOTANICAL GARDEN