Unlock instant, AI-driven research and patent intelligence for your innovation.

Mesenchymal stem cell culture medium and culture medium optimization method based on DOE experimental design

A mesenchymal stem cell and culture medium technology, applied in the field of stem cell culture, can solve the problems of low number of living cells, serum-free culture of umbilical cord mesenchymal stem cells, poor effect of mesenchymal stem cell expansion, etc.

Pending Publication Date: 2022-04-12
北京唐颐惠康生物医学技术有限公司 +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the basal medium supplemented with fetal bovine serum is most commonly used for in vitro expansion of mesenchymal stem cells; there are also serum-free cultures of human umbilical cord mesenchymal stem cells without fetal bovine serum; this type of medium is used for mesenchymal stem cells. In the case of stem cells, there is also the problem of poor expansion of mesenchymal stem cells, such as low number of viable cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mesenchymal stem cell culture medium and culture medium optimization method based on DOE experimental design
  • Mesenchymal stem cell culture medium and culture medium optimization method based on DOE experimental design
  • Mesenchymal stem cell culture medium and culture medium optimization method based on DOE experimental design

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0043] The application will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0044] 1. This application optimizes the mesenchymal stem cell culture medium based on the DOE experimental design.

[0045] The experimental raw materials and reagents are shown in Table 1, and the instruments and equipment are shown in Table 2.

[0046] Table 1 Experimental materials and reagents

[0047]

[0048] Among them, the starting water QDS and starting water QDY were donated by Jinghuan Particle Energy Technology Development Co., Ltd. The product model of the starting water QDS is Q / SHTM0001S, its product performance parameter is PH: 7.3, and its energy is low. The magnetization intensity of the composite material used is 7A / m, the magnetization time is 120h, and the density of water is 1.002g / cm 3 , The diameter of the small molecule water cluster is 1.2nm. The product model of starting water QDY is Q / XGTS01S-2012, its product perf...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of stem cell culture, and particularly discloses a mesenchymal stem cell culture medium and a culture medium optimization method based on DOE experimental design. The mesenchymal stem cell culture medium comprises the following components: a basic culture medium, at least one of starting water QDS and starting water QDY is added into the basic culture medium, the volume percentage content of the starting water QDS in the basic culture medium is 3%-12%, and the volume percentage content of the starting water QDY in the basic culture medium is 3%-12%. The mesenchymal stem cell culture medium disclosed by the invention can be used for culturing mesenchymal stem cells and has the advantage of obtaining more living cells.

Description

technical field [0001] This application relates to the technical field of stem cell culture, and more specifically, it relates to a medium for mesenchymal stem cells and a method for optimizing the medium based on DOE experimental design. Background technique [0002] Mesenchymal stem cells (MSCs) have self-renewal ability, and can differentiate into hematopoietic cells, bone cells, adipocytes, chondrocytes, muscle cells, etc. under specific culture conditions, and can also differentiate into neural cells across the germ layer boundary. cells, liver cells, islet cells, etc. Mesenchymal stem cells are mainly isolated from bone marrow at first, and then can also be obtained from tissues such as bone, fat, amniotic membrane, placenta, umbilical cord, umbilical cord blood, dental pulp, tendon and skeletal muscle. Mesenchymal stem cells have the advantages of a wide range of sources, easy isolation, rapid expansion, and low immunogenicity and immune regulation capabilities, so t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/0775
Inventor 曹毓琳田猛滕睿頔
Owner 北京唐颐惠康生物医学技术有限公司