Test method for acute toxicity of rice pest control chemicals to Procambarus clarkii
A technology of Crayfish and acute toxicity, which is applied in the field of rice pest control, can solve the problems of rice yield reduction and crayfish yield impact, etc., and achieves improved mixing precision, work efficiency and experimental result accuracy, and experimental efficiency. Effect
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Embodiment 1
[0029] The method for testing the acute toxicity of rice pest control agents to Procambarus clarkii comprises the following steps:
[0030] S1. Cultivation of Procambarus clarkii: The test Procambarus clarkii was cultivated in fresh water for 3 days with feed containing 42% crude protein, 7.5% crude fat, and 23% sodium, and the balance was carbohydrates. Dead shrimp, the body weight of Procambarus clarkii after cultivating 3d is 13g, and body length is 6cm;
[0031] S2. Water quality improvement: the test water was aerated for 24 hours, the water hardness was adjusted to 147mg / L, the water pH was 7.82, and the improved test water was obtained;
[0032]S3. Preparation of the test solution: Weigh an appropriate amount of the test medicament and dissolve it with distilled water and set the volume to a 100mL volumetric flask. , 4mL and 10mL of the mother solution were added to 1L of the improved test water obtained in step S2, and stirred for 15-30min to obtain the test agent who...
Embodiment 2
[0036] Present embodiment provides the used test device of step S4 formal test on the basis of embodiment 1, as Figure 2-5 As shown, the test device includes 2 sets of mixing test containers 1 at the bottom and 4 sets of single-component test containers 6 at the top. The tops of the 2 sets of mixing test containers 1 are connected by a set of connecting rods 2. Rod 3, the end of telescopic rod 3 is provided with 4 groups of connecting rods 4, the angle between two adjacent groups of connecting rods 4 is 90°, and the end of each group of connecting rods 4 is provided with a fixing ring 5 for fixing the single-component test container 6 , the bottom of the single-component test container 6 is provided with a water baffle 7 on the side opposite to the telescopic rod 3, and the water baffle 7 is slidably connected with the chute 61 provided at the bottom of the single-component test container 6, and the chute 61 is provided with a water outlet 62, the end of the water retaining p...
Embodiment 3
[0043] The difference between this example and Example 1 lies in that the culture conditions of Procambarus clarkii in step S1 are different.
[0044] S1. Cultivation of Procambarus clarkii: The test Procambarus clarkii was cultivated in fresh water for 3 days with feed containing 40% crude protein, 7% crude fat, and 20% sodium, and the balance was carbohydrates. Dead shrimp, Procambarus clarkii after 3 days of cultivation have a body weight of 11 g and a body length of 5.8 cm.
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