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Test method for acute toxicity of rice pest control chemicals to Procambarus clarkii

A technology of Crayfish and acute toxicity, which is applied in the field of rice pest control, can solve the problems of rice yield reduction and crayfish yield impact, etc., and achieves improved mixing precision, work efficiency and experimental result accuracy, and experimental efficiency. Effect

Active Publication Date: 2022-06-24
NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the latest statistics from the "China Crayfish Industry Development Report (2018)", the national Procambarus clarkii aquaculture area has grown to 800,000 km 2 , with an output of 1.2 million tons (but the average output is only 100kg / 667m 2 ), the reason for this phenomenon is that the rice pest control pesticides will have a certain impact on the yield of crayfish, and if no rice pest control pesticides are applied, the rice yield will decrease

Method used

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  • Test method for acute toxicity of rice pest control chemicals to Procambarus clarkii
  • Test method for acute toxicity of rice pest control chemicals to Procambarus clarkii
  • Test method for acute toxicity of rice pest control chemicals to Procambarus clarkii

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The method for testing the acute toxicity of rice pest control agents to Procambarus clarkii comprises the following steps:

[0030] S1. Cultivation of Procambarus clarkii: The test Procambarus clarkii was cultivated in fresh water for 3 days with feed containing 42% crude protein, 7.5% crude fat, and 23% sodium, and the balance was carbohydrates. Dead shrimp, the body weight of Procambarus clarkii after cultivating 3d is 13g, and body length is 6cm;

[0031] S2. Water quality improvement: the test water was aerated for 24 hours, the water hardness was adjusted to 147mg / L, the water pH was 7.82, and the improved test water was obtained;

[0032]S3. Preparation of the test solution: Weigh an appropriate amount of the test medicament and dissolve it with distilled water and set the volume to a 100mL volumetric flask. , 4mL and 10mL of the mother solution were added to 1L of the improved test water obtained in step S2, and stirred for 15-30min to obtain the test agent who...

Embodiment 2

[0036] Present embodiment provides the used test device of step S4 formal test on the basis of embodiment 1, as Figure 2-5 As shown, the test device includes 2 sets of mixing test containers 1 at the bottom and 4 sets of single-component test containers 6 at the top. The tops of the 2 sets of mixing test containers 1 are connected by a set of connecting rods 2. Rod 3, the end of telescopic rod 3 is provided with 4 groups of connecting rods 4, the angle between two adjacent groups of connecting rods 4 is 90°, and the end of each group of connecting rods 4 is provided with a fixing ring 5 for fixing the single-component test container 6 , the bottom of the single-component test container 6 is provided with a water baffle 7 on the side opposite to the telescopic rod 3, and the water baffle 7 is slidably connected with the chute 61 provided at the bottom of the single-component test container 6, and the chute 61 is provided with a water outlet 62, the end of the water retaining p...

Embodiment 3

[0043] The difference between this example and Example 1 lies in that the culture conditions of Procambarus clarkii in step S1 are different.

[0044] S1. Cultivation of Procambarus clarkii: The test Procambarus clarkii was cultivated in fresh water for 3 days with feed containing 40% crude protein, 7% crude fat, and 20% sodium, and the balance was carbohydrates. Dead shrimp, Procambarus clarkii after 3 days of cultivation have a body weight of 11 g and a body length of 5.8 cm.

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Abstract

The invention discloses a method for testing the acute toxicity of rice pest control agents to Procambarus clarkii, relates to the technical field of rice pest control, and comprises the following steps: S1, cultivation of Procambarus clarkii; S2, water quality improvement; S3, preparation of a test solution ; S4, formal experiment; S5, data analysis. The method for testing the acute toxicity of the rice disease and pest control agent of the present invention to Procambarus clarkii is under specified conditions, with 168h as the test period, Procambarus clarkii is exposed to a certain concentration of the test substance aqueous solution, according to each treatment group crayfish The impact on the viability and survival rate, compared with the blank control group, is there any difference. Prevention and control agents with high safety were preliminarily screened out.

Description

technical field [0001] The invention relates to the technical field of rice pest control, in particular to a method for testing the acute toxicity of rice pest control agents to Procambarus clarkii. Background technique [0002] The scientific name of crayfish is Procambarus clarkii. It is an aquatic animal of Crustacea, Decapod, and Crayfish. It is a freshwater economical shrimp. It is widely welcomed by people because of its delicious meat. Because of its omnivorous nature, fast growth rate and strong adaptability, it forms an absolute competitive advantage in the local ecological environment. The national Procambarus clarkii farming areas are mainly concentrated in the middle and lower reaches of the Yangtze River such as Hubei, Hunan, Jiangxi, Anhui, and Jiangsu. In recent years, areas rich in water resources in Zhejiang, Sichuan, Guangxi, Shandong, Fujian, Henan and other provinces have developed rapidly. The farming modes mainly include co-cultivation of rice and shr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50
Inventor 王宏伟田丰朱昱璇蔡小宇徐漫闫新利廖建华
Owner NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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