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Method for preparing ZZ genotype Chinese softshell turtle pseudo female individual by using non-hormone compound

A kind of Chinese soft-shelled turtle, genotype technology, applied in the direction of animal husbandry, etc., to achieve the effect of low drug residue, obvious effect and simple operation

Active Publication Date: 2022-04-15
ZHEJIANG WANLI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The problem to be solved by the present invention is how to develop a method for inducing ZZ pseudo-female soft-shelled turtles with green ecology, good sex control effect, little environmental pollution and strong operability

Method used

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  • Method for preparing ZZ genotype Chinese softshell turtle pseudo female individual by using non-hormone compound
  • Method for preparing ZZ genotype Chinese softshell turtle pseudo female individual by using non-hormone compound
  • Method for preparing ZZ genotype Chinese softshell turtle pseudo female individual by using non-hormone compound

Examples

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Embodiment 1

[0024] This example is a method for preparing ZZ genotype Chinese soft-shelled turtle pseudo-female individuals using non-hormonal compounds. The specific agonist 1,3,5-tris(4-hydroxyphenyl)-4-propyl-1H-pyrazole Induced, the chemical formula is C 24 h 22 N 2 o 3 , CAS No.: 263717-53-9, has the characteristics of green ecology, high sex reversal rate, less environmental pollution and strong operability. Specific steps include:

[0025] S1. Dissolve the specific agonist in 98% alcohol solvent, mix well to obtain an inducer with a specific agonist concentration of 2 μg / μL, and the specific agonist is 1,3,5-tris(4-hydroxyphenyl) -4-Propyl-1H-pyrazole. Collect soft-shelled turtle embryos, select well-fertilized embryos, place the selected embryos in sterilized yellow sand, and hatch at a temperature of 25-33°C and a relative air humidity of 70%-90%. When hatching to the 15.5th stage, determine the position of the air cell of the embryo by flashlight, so that the air cell face...

Embodiment 2

[0033] This example is a method for preparing ZZ genotype Chinese soft-shelled turtle pseudo-female individuals using non-hormonal compounds. The specific agonist 1,3,5-tris(4-hydroxyphenyl)-4-propyl-1H-pyrazole Inducing the feminization of ZZ Chinese soft-shelled turtle, the specific steps include:

[0034] S1. Dissolve the specific agonist in the dimethyl sulfoxide solvent, mix well to obtain the inducer with a specific agonist concentration of 2 μg / μL, and the specific agonist is 1,3,5-tris(4-hydroxybenzene base)-4-propyl-1H-pyrazole, the chemical formula is C 24 h 22 N 2 o 3 . Taking the preparation of 1 mL of inducer with a concentration of 2 μg / μL as an example, take 20 μL of the DMSO inducer mother solution with a concentration of 100 mg / mL and add it to 400 μL of PEG300, mix well; add 50 μL Tween-80 to the above system, mix uniform; then continue to add 530 μL of normal saline to dilute to 1 mL, and the content of D MSO in the inducer obtained by this method is 2%...

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Abstract

The invention provides a method for preparing ZZ genotype Chinese softshell turtle pseudo-female individuals by using non-hormone compounds, which comprises the following steps: S1, collecting Chinese softshell turtle embryos, and incubating to the 15.5 period (referring to the development staging standard of Chinese softshell turtle embryos) under the incubation conditions that the temperature is 25-33 DEG C and the relative air humidity is 70%-90%) to obtain the pseudo-female individuals of the ZZ genotype Chinese softshell turtle embryos; 3-5 [mu] L of an inducer is injected into the embryo with a microsyringe, and the effective component of the inducer is a specific agonist 1, 3, 5-tri (4-hydroxyphenyl)-4-propyl-1H-pyrazole; s2, after injecting the inducer, hatching according to the hatching condition same as that in the step S1 until the young turtles come out of shells; s3, when the embryos develop to the 27 stage, collecting gonads, carrying out histological analysis, and identifying the gender of the Chinese softshell turtles; and S4, identifying the ZZ or ZW genotype of the Chinese softshell turtles, and analyzing the number and proportion of ZZ pseudo female softshell turtles. The invention provides a brand new ZZ pseudo female soft-shelled turtle induction method which has the characteristics of being green, ecological, high in sex reversal rate and small in environmental pollution.

Description

technical field [0001] The invention relates to the technical field of aquatic product cultivation, in particular to a method for preparing ZZ genotype Chinese soft-shelled turtle pseudo-female individuals by using non-hormonal compounds. Background technique [0002] Chinese soft-shelled turtle is one of the famous and high-quality aquaculture species in southern my country. During the breeding process, there are typical female and male dimorphism. Studies have confirmed that the sex determination of soft-shelled turtles is hereditary sex determination, which belongs to the ZZ / ZW sex determination category, that is, the female soft-shelled turtle has the ZW chromosome and the male soft-shelled turtle has the ZZ chromosome. In production, male soft-shelled turtles grow fast, have thick skirts, and their economic value is higher than that of females. Studies have confirmed that during embryonic development, that is, the critical period of sex determination, the sex of soft-s...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCY02A40/81
Inventor 葛楚天郭银孙伟李攀钱国英王伟
Owner ZHEJIANG WANLI UNIV