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Preparation method and application of autologous fatty acid protein milk

A technology of fatty acid and protein milk, applied in the field of preparation of autologous fatty acid protein milk, can solve the problem that the utilization value of adipose tissue is not reflected in the clinic, and achieve the effect of promoting repair and reducing pain.

Pending Publication Date: 2022-04-15
广州安立美健康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because adipose tissue contains a variety of active ingredients, such as exosomes and extracellular matrix, which can release a variety of growth factors for tissue repair, the utilization value of adipose tissue is far from being reflected in the clinic

Method used

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  • Preparation method and application of autologous fatty acid protein milk
  • Preparation method and application of autologous fatty acid protein milk
  • Preparation method and application of autologous fatty acid protein milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of preparation method of autologous fatty acid protein milk

[0029] Take 200g of human adipose tissue, first remove the larger cord structure in the adipose tissue, let it stand to remove swelling fluid or centrifuge to remove water, and then mix the adipose tissue with PRP-platelet rich plasma at a solid-liquid ratio of 2:1 mg / mL The tissue mixture was obtained, and the tissue mixture was ground for 3 minutes. The tissue grinding condition was 50 Hz, 40 s, and 3 cycles until the tissue mixture reached a homogeneous milky white color. The ground tissue mixture was placed in an ultrasonic breaker, and the tissue The mixture is placed in a centrifuge tube, and the centrifuge tube is placed in ice cubes for ultrasonic emulsification, and the amplitude of the ultrasonic vibration is set to 50 Hz for ultrasonic emulsification, so as to obtain the autologous fatty acid protein milk product.

Embodiment 2

[0031] A kind of preparation method of autologous fatty acid protein milk

[0032] Take 200g of human adipose tissue, first remove the large cord structure in the adipose tissue, let it stand to remove swelling fluid or centrifuge to remove water, and then mix the adipose tissue and active components at a solid-liquid ratio of 4:7 mg / mL to obtain the tissue mixture,

[0033] The mixing volume ratio of 10% glutathione and 10% tranexamic acid in the active component is 2:5;

[0034] The obtained tissue mixture was ground for 1 min. The tissue grinding condition was 60 Hz, 30 s, and 3 cycles until the tissue mixture reached a homogeneous milky white color. The ground tissue mixture was placed in an ultrasonic breaker, and the tissue mixture was placed in a In a centrifuge tube, the centrifuge tube is placed in ice cubes for ultrasonic emulsification, and the amplitude of ultrasonic vibration is set to 40 Hz for ultrasonic emulsification to obtain autologous fatty acid protein mi...

Embodiment 3

[0036] A kind of preparation method of autologous fatty acid protein milk

[0037] Take 200g of human adipose tissue, first remove the larger cord structure in the adipose tissue, let it stand to remove swelling fluid or centrifuge to remove water, and then mix the adipose tissue with the active component at a solid-liquid ratio of 3:10 mg / mL to obtain the tissue mixture,

[0038] The mixing volume ratio of PRP-platelet-rich plasma, 15% glutathione and 15% tranexamic acid in the active component is 2:3:5;

[0039] The obtained tissue mixture was ground for 3 min, and the tissue grinding condition was 60 Hz, 40 s, 4 cycles, until the tissue mixture reached a homogeneous milky white color, and the ground tissue mixture was placed in an ultrasonic breaker, and the tissue mixture was Put it in a centrifuge tube, place the centrifuge tube in ice cubes for ultrasonic emulsification, set the amplitude of ultrasonic vibration to 50 Hz for ultrasonic emulsification, and obtain the aut...

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Abstract

The invention belongs to the technical field of adipose tissue modification, and particularly relates to a preparation method and application of autologous fatty acid protein milk. The method comprises the following steps: taking adipose tissue, removing a strip structure of fat, standing to remove swelling liquid or centrifugally removing moisture, mixing the adipose tissue and an active component to obtain a tissue mixture, grinding the tissue mixture, and then carrying out ultrasonic emulsification to obtain a chylous product, namely the autologous fatty acid protein milk. The active component comprises one or a mixture of more than one of PRP (Platelet Rich Plasma), glutathione or tranexamic acid. The preparation method comprises the following steps of: breaking the cell structure of the adipose tissue without complete cell structure, and releasing active substances in the adipose tissue cells through grinding and emulsifying operation to obtain a mixture of water, fatty acid and protein, namely obtaining the autologous fatty acid protein milk with a relatively complete extracellular matrix structure. Various growth factors in adipose tissue cells are released, so that tissue repair is carried out.

Description

technical field [0001] The invention belongs to the technical field of adipose tissue modification, and specifically relates to a preparation method and application of autologous fatty acid protein milk. Background technique [0002] As early as 1933, some doctors began to try to insert fat into the hip joint capsule to relieve the pain of the patient's buttocks. However, due to the immature transplantation technology, the patient's pain did not decrease but increased. Later, in 1935, Smith et al. improved the method of fat grafting and fixed the graft in a specific space. This improvement achieved good clinical results. Since then, fat grafting has gradually been applied clinically and has become popular. In the process of fat transplantation, there are more or less absorption problems of fat cells. Besides the first time, the complications of fat transplantation are also problems that need to be avoided in today's fat transplantation, such as death, stroke, vision loss, s...

Claims

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Application Information

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IPC IPC(8): A61K9/10A61K47/42A61K47/12A61K35/19A61K35/16A61K38/06A61K31/195A61P17/00A61P17/02
CPCA61K9/10A61K47/42A61K47/12A61K35/19A61K35/16A61K38/063A61K31/195A61P17/00A61P17/02
Inventor 吴引弟李清扬袁贤琳李升红高海霞
Owner 广州安立美健康生物科技有限公司
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