Quantitative phase imaging method and system based on multi-focal multiplexing lens

Abstract

The invention discloses a quantitative phase imaging method and system based on a multifocal multiplexing lens, and belongs to the technical field of optical imaging and microscopy. The method comprises the following steps: step 1, generating an incident laser beam, collimating the incident laser beam to form a collimated laser beam, and irradiating the collimated laser beam on a sample to be detected; 2, performing optical microscopic amplification on the illuminated sample to be detected, and adjusting an imaging view field on an imaging surface; 3, relaying the image with the adjusted imaging view field through a 4F system, modulating the image through a liquid crystal spatial light modulator loaded with a multifocal multiplexing lens phase model, and imaging the image on a CCD camera, the liquid crystal spatial light modulator being arranged on the Fourier surface of the 4F system; and 4, recovering the phase of the to-be-detected sample from the image imaged on the CCD camera through a light intensity transmission equation. According to the method and the system, simultaneous imaging of multiple out-of-focus intensity images of a single frame can be achieved on one CCD camera, high flexibility is achieved, and high-speed real-time phase imaging of dynamic samples such as biological cells can be achieved.

Description

technical field [0001] The invention belongs to the field of optical imaging and microscopy technology, and more specifically relates to a quantitative phase imaging method and system based on a multi-focus multiplexing lens. Background technique [0002] Quantitative phase recovery based on the light intensity transmission equation is a non-interferential phase imaging method, so it does not require complex optical path structure and phase unwrapping calculations, and is widely used in optical measurement and biomedical imaging and other fields. To solve the light intensity transmission equation, it is necessary to obtain multiple defocused intensity maps to estimate the axial intensity difference. Traditional axial scanning is generally realized by moving the CCD, but mechanical movement will not only introduce additional errors but also reduce the phase imaging system. Acquisition speed. [0003] For this reason, some researchers have proposed to use special optical devi...

AI Technical Summary

Problems solved by technology

The number of out-of-focus images collected by the former has a large limit, depending on the hardware configuration, and its de-focus distance is inconvenient to adjust flexibly. Although the latter can control the spatial light modulator to adjust the de-focus amount, its single-acquisition The total number of out-of-focus images is only 2, which is easy to reduce the accuracy of intensity axial difference

Images