Micro-liquid sample introduction device for centrifugal tube and use method of micro-liquid sample introduction device

Abstract

The invention discloses a micro-liquid sample introduction device for a centrifugal tube and a use method thereof, the micro-liquid sample introduction device comprises a base and an upper cover, the base is detachably connected with the upper cover; a stepped hole is formed in the base in the axis direction, the centrifugal tube is sleeved and fixed in the stepped hole, a plunger is arranged on one side of the upper cover, a sealing ring is fixed on the outer surface of the plunger, and the sealing ring and the inner wall of the centrifugal tube are extruded to deform, so that the centrifugal tube is sealed; the upper cover is further provided with a gas path channel communicated with the axial cavity, one end of the gas path connector is connected with a pressure source, the other end of the gas path connector is communicated with the cavity, and the micro sample injection needle is fixed in the cavity in the axial direction; the sample introduction device can be used for directly transferring a sample to be detected in a consumable, so that the automation level is improved, the operation difficulty, the volume and the cross contamination risk are reduced, and the technical problem in the field of trace sample transfer is solved.

Description

technical field [0001] The invention relates to the field of trace liquid analysis equipment, in particular to a trace liquid sampling device for a centrifuge tube and a use method thereof. Background technique [0002] Currently, the methods used for micro-fluid loading in vitro diagnostics include manipulators, lateral flow chromatography, capillary electrophoresis, centrifugal force, syringe pumps, peristaltic pumps, pressure pumps and other methods, the core of which is to provide power to drive the liquid to a designated position. Among them, the manipulator is used to adjust the position of the sample loading tube, or push the piston and squeeze the liquid storage chamber to load the sample. Lateral chromatography is only suitable for chromatographic reactions in test strips, capillary electrophoresis is mostly suitable for charged samples such as nucleic acids, and centrifugal force is limited to butterfly chips, so it has a specific scope of application and applicati...

AI Technical Summary

Problems solved by technology

Common gas pressurization usually puts the solution in a pressure vessel that is used repeatedly for pressurization. The volume of the pressure vessel is often much larger than the volume of the solution to be tested, and considering the requirements of sealing, there are complex sealing designs, which lead to its installation and the increased difficulty of using

like figure 1 and figure 2 The conventional gas pressurization device shown has obvious hysteresis in pressure switching due to its large volume, and the accuracy is not high

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