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Fermentation process and culture medium of acremonium terricola

A fermentation medium and a fermentation process technology are applied in the field of the fermentation process and culture medium of Acremonium terrestris, and can solve the problems of unsuitability for large-scale industrial production, low content of adenosine in culture, low content of active ingredients, etc. The effect of promoting rapid mass reproduction, simple fermentation process and increasing content

Pending Publication Date: 2022-05-06
张小朋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that in the prior art, the fermentation time of Acremonium acremonium is long, the adenosine content of the culture is low, the process of obtaining the culture in the later stage is cumbersome, the cost and energy consumption are high, and the content of active ingredients is low, there are many defects, and it is not suitable for large-scale factories. Production needs to be improved urgently

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Acremonium acremonium was fermented using the following process:

[0033] (1) Acremonium acremonium was inoculated into the slant medium, and cultured at 25.0±2°C for 6 days to obtain the slant strain; the slant medium used was PDA solid medium;

[0034] (2) The slant strains were cultured in shake flasks and cultured in primary shake flasks, then transferred to secondary shake flasks for seed culture, and cultured at 25.0±2°C for 3 days to obtain secondary shake flask culture fluid;

[0035] (3) Inoculate the secondary shake flask culture medium with 2% inoculation amount into the fermenter containing the primary seed medium for fermentation. During the fermentation process, the culture temperature was controlled at 25.0±2°C, the stirring speed was controlled at 220r / min, and the air The flow rate is controlled at 1:1V / V / min, cultured for 1d, and the first-grade seed culture solution is obtained;

[0036] (4) After the pH of the first-level seed culture solution drops...

Embodiment 2

[0050] Adopt same substratum, i.e. the substratum formula of experimental group 4 among the embodiment 1, different fermentation conditions ferment Acremonium acremonium, concrete experimental grouping is as follows:

[0051] 1. Experimental group 7: adopt the fermentation condition described in embodiment 1.

[0052] 2. Experimental group 8:

[0053] (1) Acremonium acremonium was inoculated into the slant medium, and cultured at 25.0±2°C for 6 days to obtain the slant strain, and the slant medium used was PDA solid medium;

[0054] (2) Culture the slant bacteria in the primary shake flask, then transfer to the secondary shake flask for seed cultivation, and cultivate at 25.0±2°C for 3 days to obtain the secondary shake flask culture solution;

[0055] (3) Inoculate the secondary shake flask culture medium with 2% inoculation amount into the fermenter containing the primary seed medium for fermentation. During the fermentation process, the culture temperature was controlled a...

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Abstract

The invention discloses a fermentation process and a culture medium of acremonium terricola, which not only can promote the growth of the acremonium terricola, but also can significantly increase the content of metabolites such as adenosine and the like and improve the use value of the acremonium terricola culture by scientifically adjusting the content of each nutrient substance in the fermentation culture medium and accurately controlling the fermentation process.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a fermentation process and a culture medium of acremonium acremonium. Background technique [0002] Acremonium acremonium is a kind of Cordyceps fungus, which is mostly parasitic in Cordyceps, and can synthesize a variety of pharmacologically active substances. Its mycelia and cultures contain many types of pharmacologically active ingredients. After testing, these pharmacologically active ingredients have Antitumor activity and the function of enhancing immune activity in animals, so it can be used in the preparation of feed additives, medicines and health foods. [0003] Acremonium acremonium culture is a product obtained by artificial fermentation of Acremonium acremonium isolated from Cordyceps guni. It has functional components similar to Cordyceps guni, such as cordycepin, cordycepic acid, polysaccharide, adenosine, etc., artificially fermented The cost ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/40C12R1/645
CPCC12N1/14C12P19/40
Inventor 张小朋李晓军黄福岚赵佳余有龙王丁相
Owner 张小朋
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