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Tissue culture method of salix babylonica

A technology of tissue culture and inoculation medium, which is applied in the field of plants, can solve the problems of original vegetation damage and unfavorable protection of original vegetation, and achieve the effect of reducing damage

Pending Publication Date: 2022-05-10
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the yellow willow cuttings required for cutting need to cut a large number of adult wicker, it is easy to cause damage to the original vegetation, which is not conducive to the protection of the original vegetation

Method used

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  • Tissue culture method of salix babylonica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] explant disinfection

[0026] The terminal buds and axillary buds on the young shoots of the year were selected as explants. First take the tender branches of the yellow willow, put the young branches into a beaker, add a few drops of detergent, add water, rinse under tap water until there is no foam, and repeat once. The tap water washing time should not be too long to avoid secondary pollution of the explants. Get the ultra-clean workbench to prepare for disinfection and vaccination. Use scissors to cut the twigs to 3-4cm long and put them in a grinding bottle for disinfection, soak them in 70% alcohol for 1 minute in the ultra-clean bench, rinse them twice with sterile water, and then use 0.2% mercuric chloride (Add two drops of Tween) to disinfect for about 10 minutes, and finally wash 6 times with sterile water.

[0027] culture medium

[0028] Cut off excess leaves and stem segments, keep 1.5-2.0cm stem segments with terminal buds and lateral buds for inoculat...

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Abstract

The invention discloses a tissue culture method of salix babylonica. According to the tissue culture propagation method, mass propagation of the wicker is realized by using fewer plant branches. According to the method, damage to existing vegetation can be reduced, technical support is provided for large-scale production of desertification control materials, and the method has important application value.

Description

technical field [0001] The invention belongs to the field of plants and relates to a method for tissue culture of yellow willow. Background technique [0002] Yellow willow (Salix gordejevii) is a deciduous and clumped shrub of Salix genus Salix, which is generally distributed on the flowing and semi-moving sandy land in the grassland belt. Because yellow willow is adaptable to quicksand and has strong germination ability, it is easy to grow adventitious roots and buds under quicksand burial, forming new branches and root systems. It is easy to reproduce asexually and has strong wind resistance. It is an excellent pioneer sand-fixing species. It is used in artificial sand control. Widely used in the field, the effect is good. Within a certain range, the growth rate of yellow willow clusters is positively correlated with the depth of sand burial. As long as the sand burial does not exceed the canopy, the thicker the sand layer, the more vigorous the growth will be, and event...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
CPCA01H4/001A01H4/008A01G31/00
Inventor 白永飞王扬
Owner INST OF BOTANY CHINESE ACAD OF SCI
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