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Method for detoxification of lily through somatic embryogenesis

An embryogenesis and somatic cell technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of complicated shoot tip culture operation, easy browning of shoot tip, incomplete detoxification, etc. Cultivation time, good detoxification effect, and the effect of mass reproduction

Active Publication Date: 2015-09-16
CHONGQING LANDSCAPE & GARDENING RES INST +1
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  • Abstract
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  • Claims
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Problems solved by technology

The shoot tip culture operation is complicated, the shoot tip is prone to browning after inoculation, and the survival rate is low
Therefore, the traditional detoxification method needs to carry out physical or chemical inhibitory treatment on the test materials. The success of detoxification is related to the size of the artificially excised meristem. It is greatly affected by environmental and human factors, and the detoxification is not complete. The problem

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  • Method for detoxification of lily through somatic embryogenesis

Examples

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Effect test

Embodiment 1

[0024] The method for lily detoxification through the somatic embryogenesis pathway comprises the following steps:

[0025] 1) Embryogenic callus induction: Lily scales cultivated under sterile conditions were used as explants, inoculated on solid medium for embryogenic callus induction, and cultured for 28 days to induce embryogenic callus.

[0026] Among them, the composition of embryogenic callus induction medium is: MS medium + PIC 1 ~ 3mg L -1 +Agar 7g·L -1 + sucrose 30g·L -1 , PH value is 5.8. PIC is one of the plant growth regulators, its full name in English is Picloram, and its Chinese name is picloram.

[0027] The embryogenic callus induction medium is to add 7g·L -1 Agar and 30g·L -1 Sucrose, maintain a pH of 5.8, and add the following ingredients:

[0028] PIC (mg·L -1 ) BA (mg·L -1 ) NAA (mg·L -1 ) Effect Recipe 1 1 0 0 Fewer calluses formed at the base of the scales, accompanied by a small amount of differentiation into smal...

Embodiment 2

[0035] In this example, step 1) and step 2) are the same as in Example 1, and step 3) is the subculture of spherical somatic embryos in a bioreactor: inoculate the spherical somatic embryos obtained in step 2) in Example 1 The first-generation subculture was carried out in a bioreactor added with liquid proliferation medium, and the mass-volume concentration of spherical somatic embryos inoculated in each bioreactor was 0.5-5% (w / v), that is, each Add 100ml of liquid proliferation medium to a bioreactor to inoculate 0.5~5g of spherical somatic cell embryos. In this embodiment, 1 g of spherical somatic cell embryos was inoculated in each bioreactor, and the air flow of the bioreactor was adjusted to 20 ml min after inoculation. -1 , the temperature was kept at 24±2°C, and cultured for 14 days under dark and light culture conditions to obtain the first generation of subcultured somatic cell embryos.

[0036] The newly produced spherical somatic embryos were screened out from th...

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Abstract

The invention discloses a method for detoxifying lily by means of somatic embryogenesis. The method comprises the following steps: (1) performing embryonic callus induction, namely inducing embryonic calluses by taking lily scales, cultured under an aseptic condition, as explant; (2) performing selective subculture on embryonic calluses, namely selecting a new spherical somatic embryo, produced by the embryonic calluses, as a subculture material; and (3) performing subculture of the spherical somatic embryo, namely inoculating a solid proliferation culture medium or a liquid proliferation culture medium with the spherical somatic embryo for performing subculture till no virus is detected in the subcultured spherical somatic embryo. The operating method is simple, repeated subculture is performed on a to-be-detoxified material by an ordinary tester, and the requirements on test conditions and operating workers are low. Through rapid proliferation of the somatic embryo, complete detoxification can be realized, and a good detoxification effect is achieved.

Description

technical field [0001] The invention relates to the technical field of lily detoxification breeding, in particular to a method for detoxification of lily through somatic embryogenesis. Background technique [0002] As an auspicious flower, lily is deeply loved by people because of its meaning of a hundred years of harmony. Perfume lily is the queen of lilies. Because of its pleasant fragrance, it is the best among fresh cut flowers and the first choice for gifting. In recent years, lily cut flowers are a rookie after the world's top five cut flowers (rose, carnation, chrysanthemum, gladiolus, gerbera), and are currently one of the most popular cut flowers in the world. However, the biggest problem in the lily planting process is the degeneration of lily varieties - virus is one of the main reasons. In lilies, there are more than ten kinds of common viruses such as Cucumber Mosaic Virus (CMV), Tulip Breaking Virus (TBV), Lily Symptomless Virus (LSV), etc. These viruses It ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李玲莉孔立生刘华敏
Owner CHONGQING LANDSCAPE & GARDENING RES INST
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