Determination method for gene drift frequency of non-selectable marker transgenic rice

Abstract

The invention provides a method for measuring the gene drift frequency of non-selectable marker transgenic rice, which comprises the following steps: acquiring and planting transgenic rice and gene drift receptors, calculating the germination rate of the gene drift receptors, taking seeds, and culturing to obtain hybrid seedlings after the seeds are taken; designing a specific molecular marker according to the exogenous gene, and sequentially carrying out mixed pool detection, grouping detection and plant-by-plant detection on the hybrid seedlings through the specific molecular marker; and calculating the gene drift frequency. By adopting the regional module mixed pool sampling method, the workload is reduced, and the working efficiency is improved. The method is not influenced by selection markers, work can be carried out after harvest in season, and the actual detection quantity is obviously lower than the detection quantity required by a traditional detection method. In conclusion, the method can solve the problems that traditional transgenic rice gene drift probability calculation is large in workload and long in time consumption, and the gene drift frequency is influenced by the group scale, the full-bloom stage wind direction and the sampling space distribution.

Description

technical field [0001] This application relates to the field of ecological security assessment, in particular to a method for determining the gene drift frequency of non-selectable marker transgenic rice. Background technique [0002] It has been more than 20 years since the commercial planting of genetically modified crops in the world, and its planting area is increasing year by year and has created huge economic benefits. At present, the main transgenic crops planted in the world are corn (Zeamays), soybean (Glycinemax), cotton (Gossypium hirsutum) and rapeseed (Brassicanapus), etc. The transgenic traits of these crops mainly include herbicide resistance, virus resistance and insect resistance. In addition to genetically modified cotton, my country is also gradually promoting the commercial planting of major food crops, and has successively issued safety certificates for self-developed genetically modified rice, genetically modified soybeans and genetically modified corn....

AI Technical Summary

Problems solved by technology

[0004] This application provides a method for determining the gene drift frequency of non-selectable marker-free transgenic rice, which can solve the problem of the heavy workload and time-consuming calculation of the gene drift probability of traditional transgenic rice, and the gene drift frequency is also affected by the population size, the wind direction of the blooming period and the sampling space distribution. problem of influence

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