Tissue culture method of liquidambar formosana hance

Abstract

The invention discloses a tissue culture method of liquidambar formosana hance. The method comprises the following steps: pretreatment of a liquidambar formosana hance explant, collection of the liquidambar formosana hance explant, disinfection of the liquidambar formosana hance explant, induction culture of the liquidambar formosana hance explant, multiplication culture of a liquidambar formosana hance bud, strong seedling culture of the liquidambar formosana hance bud, and rooting culture of a liquidambar formosana hance seedling. In the whole process, the low-ammonium culture medium is adopted for culture, and the main purpose is to reduce the reversion phenomenon of the liquidambar formosana hance. According to the method, female parent shading treatment is adopted, the inducing survival rate of the liquidambar formosana hance is greatly increased, and more liquidambar formosana hance seedlings can be obtained within a short time. In the whole Liquidambar formosana Hance tissue culture, low-ammonium culture media such as basic formula WPM and Huabao No.1 are adopted, and different vitamins and organic matters are added to form different culture medium formulas, so that the requirements of nursery stocks at different stages on nutrition are met. Vitamin, malic acid, tyrosine and acid hydrolyzed casein are respectively added into each culture medium of the liquidambar formosana hance.

Description

technical field [0001] The invention relates to the technical field of plant propagation, in particular to a method for tissue culture of sweetgum silvery deer. Background technique [0002] In the field of plant propagation technology, tissue culture is one of the artificial vegetative propagation methods of plants. Liquidambar formosana H., also known as Liquidambar formosana H., is a deciduous tall tree of the genus Liquidambar L. in the family Hamamelis. The blank of cultivating tall deciduous trees with ever-colored leaves has great potential and value in garden applications. [0003] Silver Deer Sweetgum is a new plant variety, and there are very few public reports on its reproduction technology. At the same time, this tree species is a deciduous tree species. The grafting of deciduous tree species is traditionally grafted during the dormant period. Grafting in the middle dormant period is adopted; however, grafting in the dormant period is limited by factors such as...

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Problems solved by technology

[0010] In the prior art, there is no special publication on the tissue culture of Liquidambar Yinlu. In theory, the tissue culture of Liquidambar Yinlu should be an effective solution to this problem. Effective means of breeding

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