Cytochrome P450 and application thereof in degradation of pesticide residues

Abstract

The invention discloses a cytochrome P450, the amino acid sequence of the cytochrome P450 is shown as SEQ ID NO: 2, the P450 gene has an overexpression phenomenon in populations with low-level to medium-level resistance to organophosphorus in the field, and the gene can be used as a molecular marker to assist in early detection and early warning of whether the field tetranychus urticae populations have drug resistance to organophosphorus insecticides or not. And the detection process is simple and rapid. The gene can metabolize chlorpyrifos and pirimiphos-methyl in vitro, and is expected to metabolize other organophosphorus insecticides, so that engineering bacteria expressed by the gene can realize degradation of fruits, vegetables and the like with organophosphorus insecticide pesticide residues, pretreatment of wastewater and sewage, treatment of soil polluted by pesticides and the like, and have industrial development and application potential. The cloned P450 gene participating in drug resistance can also be used as a target to design an inhibitor of a specific detoxifying enzyme in a targeted manner, and a thought is provided for research and development of novel medicaments in the future.

Description

technical field [0001] The invention belongs to the fields of biotechnology and environmental protection, and in particular relates to a cytochrome P450 and its application in degrading pesticide residues. Background technique [0002] Two-spotted spider mite Tetranychus urticae Belonging to the order Acarinae of the order Arachnida, it is a major agricultural pest mite distributed worldwide. Tetranychus urticae has a complex diet and can feed on more than 1,100 species of host plants, including vegetables, fruit trees, flowers, and peanuts. Tetranychus urticae like to cluster on the back of plant leaves, and do damage by sucking plant juice in three developmental stages: adults, larvae, and nymphs. First, white spots are formed around the main veins of leaves, which in turn causes leaves to turn white and affects plant photosynthesis. In severe cases, the leaves turn yellow and dry, and even the whole plant dies, which greatly affects the yield and quality of crops. At p...

AI Technical Summary

Problems solved by technology

Tetranychus urticae like to cluster on the back of plant leaves, and do damage by sucking plant juice in three developmental stages: adults, larvae, and nymphs. First, white spots are formed around the main veins of leaves, which in turn causes leaves to turn white and affects plant photosynthesis. In severe cases, the leaves turn yellow and dry, and even the whole plant dies, which greatly affects the yield and quality of crops.

After Tetranychus urticae developed resistance to organophosphorus insecticides, the P450 genes involved in detoxification and metabolism with definite physiological functions were rarely disclosed. The lack of these key genes involved in the formation of resistance also hindered the development of new pesticides and Development of pest resistance management strategies

Based on this, the present invention has discovered that a P450 gene CYP392D8 is overexpressed in the population of resistant spider mite, and the gene can be metabolized in vitro after heterologous expression and protein purification. The P450 gene involved in the resistance of Tetranychus urticae to organophosphorus insecticides has been reported. At the same time, it also provides a theoretical basis for the design of new pesticide targets

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