Molecular marker of rice gelatinization temperature gene ALK as well as primer and application of molecular marker

A molecular marker, gelatinization temperature technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial determination/inspection, etc., can solve the problems of difficult phenotype, rough, time-consuming and laborious, and achieve accurate detection Time saving and good primer specificity
CN114480720APending Publication Date: 2022-05-13CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
Publication Date
2022-05-13

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Abstract

The invention discloses a molecular marker of a rice gelatinization temperature gene ALK as well as a primer and application of the molecular marker. Nucleotide sequences of the molecular marker are shown as SEQ ID NO.3 and SEQ ID NO.4. The nucleotide sequence of the high-alkali elimination rice amplification product is as shown in SEQ ID No.3, the nucleotide sequence of the medium-alkali elimination rice amplification product is as shown in SEQ ID No.4, and the heterozygous genotype rice material is simultaneously amplified into bands as shown in SEQ ID No.3 and SEQ ID No.4. According to the invention, the identification of the rice molecular marker assisted breeding high / medium alkali elimination value material can be rapidly detected by using a simple and reliable method.
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Description

technical field

[0001] The invention relates to a molecular marker of a rice gelatinization temperature gene, in particular to a molecular marker of a rice gelatinization temperature gene ALK, a primer and application thereof. Background technique

[0002] Rice quality mainly includes four aspects: milling quality, appearance quality, nutritional quality and cooking and eating quality. The evaluation indicators of these four quality traits cover a series of physical and biochemical characteristics. The genetic factors of rice itself are the main reason for determining these characters. Therefore, it is of great significance to study a series of genetic mechanisms of rice quality. So far, scientists have mapped QTLs for multiple genes related to rice quality, some of which have been cloned and functionally verified. Most of these cloned genes and QTLs have been developed into molecular markers. Molecular marker-assisted selection technology can improve the breeding efficien...

Claims

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