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Method for testing skin sensitization in human cell line activation test

A skin sensitization, human cell line technology, applied in biological testing, preparation of test samples, measurement devices, etc., can solve the problems of low reliability and low safety

Pending Publication Date: 2022-05-13
启真(广州)科技投资中心(有限合伙)
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Problems solved by technology

[0003] For the problems raised by the background technology, the purpose of the present invention is to propose a human cell line activation test for the test method of skin sensitization, which is an in vitro detection method with high stability and reliability, and can be applied to skin sensitization. The detection of allergens solves the problems of low reliability, low safety and the need for animal experiments in traditional skin sensitization detection methods

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  • Method for testing skin sensitization in human cell line activation test
  • Method for testing skin sensitization in human cell line activation test
  • Method for testing skin sensitization in human cell line activation test

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Embodiment Construction

[0043] Such as figure 1 Shown, a kind of human cell line activation test is used for the test method of skin sensitization, comprises the following steps:

[0044] Step S1, cell culture and cell stability testing: THP-1 suspension cells were incubated at 37°C, 5% CO 2 Under the conditions of cell culture, use 1640 complete medium to culture in the incubator, observe the state of the cells, recover the cells and pass the cell stability test for testing;

[0045] Step S2, CV75 concentration confirmation: the cells were divided into 2×10 5 The cell / mL density was cultured in the cell culture incubator for 48 hours, centrifuged, and fresh 1640 complete medium was added to adjust the cell density to 2×10 6 cells / mL, draw 500 μL cell suspension and 500 μL test substance working solution and inoculate them on a 24-well plate at a volume ratio of 1:1. The test substance working solution consists of a series of test substance stock solutions with 8 concentration points After dilutio...

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Abstract

The invention relates to the technical field of sensitization testing, in particular to a method for testing skin sensitization in a human cell line activation test. A method for testing skin allergenicity by a human cell line activation test comprises the following steps: step S1, cell culture and cell stability detection: THP-1 suspension cells are cultured in an incubator by using a 1640 complete medium under the cell culture conditions of 37 DEG C and 5% CO2, the cell state is observed, and the cells are recovered and are used for testing after passing cell stability detection; and S2, confirming the concentration of CV75: culturing the cells in a cell culture box for 48 hours at the density of 2 * 10 < 5 > cells / mL, and centrifuging. The human cell line activation test method for skin sensitization is an in-vitro detection method with high stability and reliability, can be applied to detection of skin sensitization substances, and solves the problems that a traditional skin sensitization detection method is low in reliability and safety and needs animal experiments for detection.

Description

technical field [0001] The invention relates to the technical field of sensitization testing, in particular to a test method for skin sensitization in a human cell line activation test. Background technique [0002] Allergic contact dermatitis is a delayed allergic reaction triggered by skin contact with sensitizing substances, which involves a series of reaction processes. Skin sensitization testing is an important part of the safety evaluation of skin contact products. In recent years, with the advancement of science and technology and the introduction of the "3R" principle of animal welfare and animal protection, as well as the strengthening of international supervision and the establishment of animal ethics principles How to reduce, optimize, and replace animal experiments has become the focus of special attention all over the world. Most of the current domestic standards are still at the level of animal experiments. Whether it is from the scientific point of view of de...

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Application Information

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IPC IPC(8): G01N15/10G01N21/64G01N33/50G01N1/30
CPCG01N15/10G01N21/6428G01N33/5091G01N1/30
Inventor 钟晓明袁园胥萍
Owner 启真(广州)科技投资中心(有限合伙)
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