Self-assembly method based on amino acid and near-infrared dye and anti-tumor application

Abstract

Compared with the traditional tumor treatment method, the gas therapy as an emerging and promising cancer treatment method is widely concerned. Although a few NO donors have obtained certain effects in the aspect of tumor treatment at present, the NO release strategies are still limited in application and even bring a lot of side effects. According to the present invention, the endoplasmic reticulum-targeted self-assembly multifunctional nano-composite FL (at) IR783 using Fmoc-L-arginine (Fmoc-L-Arg,) as the NO precursor is designed, such that the endoplasmic reticulum-targeted self-assembly multifunctional nano-composite FL (at) IR783 is provided; firstly, IR783 is endocytosed by tumor cells to reach ER in a targeted mode, then IR783 generates a large amount of ROS mainly containing < 1 > O2 under excitation of near infrared rays, and guanidyl of L-arginine is induced to be oxidized to generate NO. NO released in cells can inhibit activation of tumor-related platelets, enhance vascular permeability, increase accumulation of nanoparticles in tumors and improve the anti-tumor effect on one hand; on the other hand, unnecessary systematic bleeding can be prevented. In-vitro experiments show that FL at IR783 is mainly gathered on endoplasmic reticulum in cells, after near-infrared laser irradiation, not only is endoplasmic reticulum stress caused, but also generated < 1 > O2 is utilized to induce generation of NO, and then growth of tumor cells is inhibited. In a word, the research result shows that the multifunctional nano-composite has good application potential in tumor treatment.

Description

technical field [0001] The invention relates to a preparation method for self-assembly of amino acids and near-infrared dyes to form a multifunctional nanometer treatment system. Specifically, it relates to a nanocomposite that utilizes amino acids and near-infrared dyes to self-assemble and disperse in different solvents to form different states. Moreover, the nanocomposite can be used for drug delivery and tumor treatment, and belongs to the fields of nanomaterials and regenerative medicine. Background technique [0002] Gas therapy has received a lot of attention as an emerging and promising approach to cancer treatment. Nitric oxide (NO), synthesized by the nitric oxide synthase (NOS) enzyme, acts as a key signaling molecule in various physiological and pathological processes. NO has been found to play an important role in the cardiovascular system, respiratory system, nervous system, immune system, antibacterial therapy, wound healing and other biomedical fields, espe...

AI Technical Summary

Problems solved by technology

However, the application of these NO release strategies remains limited, which often involve complex synthesis, unresponsive release, and lack of specific targets, leading to excess harmful NO in normal tissue blood vessels.

Images