Application of Mitoquinone in preparation of anti-coronavirus infection medicine

A technology of coronavirus and medicine, applied in the field of application in the preparation of anti-coronavirus infection medicine

Pending Publication Date: 2022-05-17
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For the lung caused by coronavirus, respiratory and intestinal diseases, nervous system symptoms and myocarditis and other related inflammatory diseases lack effective prevention and treatment drugs, the invention provides a kind of application of Mitoquinone in the preparation of anti-coronavirus infection drugs, in order to achieve The purpose of effectively inhibiting the activity of coronavirus 3C-like protease 3Clpro

Method used

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  • Application of Mitoquinone in preparation of anti-coronavirus infection medicine
  • Application of Mitoquinone in preparation of anti-coronavirus infection medicine
  • Application of Mitoquinone in preparation of anti-coronavirus infection medicine

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Time-dependent fluorescence intensity of substrate peptides metabolized by 3C-like proteases under different inhibitor concentrations

[0032] The specific implementation process is:

[0033] 1) The SARS-Cov-2 3C-like protease and substrate peptide stock solutions are stored in a -80°C refrigerator;

[0034] 2) Thaw the SARS-Cov-2 3C-like protease in a cryopreservation plate (-4 to 4°C) at room temperature, take 1uL and dilute it in 98uL borate borax buffer (PH=7.4), and add it to the detection plate;

[0035] 3) Add 1uL of different concentrations of inhibitors (Mitoquinone, concentrations of 0, 0.05mM, 0.1mM, 0.25mM, 0.5mM, 1mM, 2.5mM) into the solution obtained in the above step (2);

[0036] 4) Add 1uL of the same concentration of substrate peptide (0.5mM) to the solution obtained in the above step (3), incubate at 37°C with a fluorescent microplate reader, and monitor the fluorescence emission at 342nm excitation and 496nm with a fluorescent microplate reader , d...

Embodiment 2

[0039] Determination of Compound Mitoquinone's Inhibitory Ability to SARS-Cov-2 3C-like Protease

[0040] The specific implementation process is as follows:

[0041] 1) The SARS-Cov-2 3C-like protease and substrate peptide stock solutions are stored in a -80°C refrigerator;

[0042] 2) Thaw the SARS-Cov-2 3C-like protease in a cryopreservation plate (-4 to 4°C) at room temperature, take 1uL and dilute it in 98uL borate borax buffer (PH=7.4), and add it to the detection plate;

[0043] 3) Add 1uL of different concentrations of inhibitors (Mitoquinone, concentrations of 0, 0.05mM, 0.1mM, 0.25mM, 0.5mM, 1mM, 2.5mM) into the solution obtained in the above step (2);

[0044]4) Add 1uL of the same concentration of substrate peptide (0.5mM) to the solution obtained in the above step (3), incubate at 37°C with a fluorescent microplate reader, and monitor the fluorescence emission at 342nm excitation and 496nm with a fluorescent microplate reader , detect while incubating, incubate f...

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PUM

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Abstract

The invention discloses an application of Mitoquinone in preparation of an anti-coronavirus infection drug and an application of Mitoquinone in preparation of an inhibitor drug of coronavirus 3C-like protease 3Clpro, the Mitoquinone has a strong inhibition effect on the enzymatic activity of the target 3C-like protease 3Clpro of the coronavirus, has the potential of being developed into a drug for treating and preventing the coronavirus, especially an anti-new coronavirus, and can be used for preparing the inhibitor drug of the coronavirus 3C-like protease 3Clpro. A new thought is provided for treating and preventing coronavirus, especially new coronavirus.

Description

technical field [0001] The invention relates to the technical field of medicinal chemistry, in particular to the application of Mitoquinone in the preparation of anti-coronavirus infection drugs. Specifically, Mitoquinone acts as a coronavirus 3C-like protease inhibitor. Background technique [0002] Coronaviruses belong to single positive-strand RNA viruses, and the coronavirus family mainly includes novel coronavirus (SARS-CoV-2), SARS coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU. Coronaviruses commonly cause respiratory and intestinal disease, neurological symptoms, and myocarditis. In 2003, severe upper respiratory disease (SARS) broke out, and about 8000 people were infected all over the world, and the mortality rate was as high as 10%. In 2014, the Middle East Respiratory Syndrome (MERS) broke out in many countries around the world, and the mortality rate was higher than that of SARS-CoV, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/662A61P31/14
CPCA61K31/662A61P31/14
Inventor 韩克利张洪苓贾燕王艺颖
Owner SHANDONG UNIV
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