Method for determining earthy smell substances in freshwater fish
A method of determination, a technology of earthy smell, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unsatisfactory sensitivity and selectivity, unsatisfactory effect, complicated devices, etc., to avoid interference of impurities and easy simultaneous determination , the effect of high accuracy
Pending Publication Date: 2022-05-20
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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[0003] In the past 30 years, many methods have been developed to determine the GSM and 2-MIB in the water environment, but there are relatively few researches on the detection methods of earthy odor substances in freshwater fish at home and abroad, although the human nose can measure the GSM and 2-MIB in the water environment. Geosmin and 2-methylisoborneol can be detected within a certain range, but only semi-quantitative data can be provided, and the problem of sensory overload is prone to occur; the electronic nose with a special sensor can detect geosmin and 2-methylisoborneol, However, the sensitivity and selectivity in complex matrices such as fish cannot meet the requirements; the microwave distillation-solid phase microextraction method is not ideal due to the complexity of the device and low recovery rate
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Embodiment 1
[0048] The method for the determination of earthy smell substances geosmin and 2-methylisoborneol in freshwater fish by n-hexane extraction-solid phase extraction concentration-gas chromatography mass spectrometry comprises the following steps:
[0049] Crucian carp, remove the scales, viscera and skin, take the muscle along the back, homogenize and mix well.
[0050] The first step, extraction: Weigh 5g of crucian carp minced meat, add 15mL of n-hexane to fully shake, ultrasonic for 15min, centrifuge at 4500r / min for 8min, take the supernatant into a 50mL centrifuge tube; then add 10mL of n-hexane to the fish, fully shake, and ultrasonically After 15 minutes, centrifuge at 4500r / min for 8 minutes, take the supernatant and put it into a 50mL centrifuge tube.
[0051] The second step, purification: add 0.5g of C18 powder, 0.5g of graphitized carbon black, and 2g of anhydrous magnesium sulfate into the centrifuge tube of the first step, shake for 7min, and centrifuge at 2500r / mi...
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The invention discloses a method for determining earthy smell substances in freshwater fish. The method comprises the following steps: 1, extracting the earthy smell substances in freshwater fish paste twice by adopting normal hexane to obtain an extracting solution; step 2, adding C18 powder, graphitized carbon black and anhydrous magnesium sulfate into the extracting solution obtained in the step 1, fully oscillating, and centrifuging to achieve a purification effect; 3, purifying and concentrating the sample extracting solution by adopting a silica gel solid-phase extraction column to obtain an eluent; and 4, determining the content of the earthy substances in the eluent obtained in the step 3 by using a gas chromatography-mass spectrometry method. The method for determining the earthy smell substances in the freshwater fish is simple and easy to operate, higher in accuracy and better in stability.
Description
technical field [0001] The invention belongs to the technical field of food detection, and relates to a method for measuring geosmin (GSM) and 2-methylisoborneol (2-MIB), which are earthy smell substances in freshwater fish. Background technique [0002] my country's freshwater fish production is very large, accounting for 65% of the world's total, and plays a key role in the effective supply of aquatic products in the market. However, because freshwater fish generally has an unpleasant earthy smell, it seriously affects the taste and economic value of freshwater fish and its processed products, and restricts the healthy development of freshwater fish farming and processing industries. The substances that produce earthy smell are mainly Geosmin and 2-methylisoborneol. In view of this situation, it is urgent to establish an effective detection method for earthy odor substances in freshwater fish to provide technical support for further research. [0003] In the past 30 year...
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Login to View More IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/062Y02A40/81
Inventor 田良良胥亚夫孔聪蔡友琼方长玲沈晓盛
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI