Method for determining earthy smell substances in freshwater fish

Abstract

The invention discloses a method for determining earthy smell substances in freshwater fish. The method comprises the following steps: 1, extracting the earthy smell substances in freshwater fish paste twice by adopting normal hexane to obtain an extracting solution; step 2, adding C18 powder, graphitized carbon black and anhydrous magnesium sulfate into the extracting solution obtained in the step 1, fully oscillating, and centrifuging to achieve a purification effect; 3, purifying and concentrating the sample extracting solution by adopting a silica gel solid-phase extraction column to obtain an eluent; and 4, determining the content of the earthy substances in the eluent obtained in the step 3 by using a gas chromatography-mass spectrometry method. The method for determining the earthy smell substances in the freshwater fish is simple and easy to operate, higher in accuracy and better in stability.

Description

technical field [0001] The invention belongs to the technical field of food detection, and relates to a method for measuring geosmin (GSM) and 2-methylisoborneol (2-MIB), which are earthy smell substances in freshwater fish. Background technique [0002] my country's freshwater fish production is very large, accounting for 65% of the world's total, and plays a key role in the effective supply of aquatic products in the market. However, because freshwater fish generally has an unpleasant earthy smell, it seriously affects the taste and economic value of freshwater fish and its processed products, and restricts the healthy development of freshwater fish farming and processing industries. The substances that produce earthy smell are mainly Geosmin and 2-methylisoborneol. In view of this situation, it is urgent to establish an effective detection method for earthy odor substances in freshwater fish to provide technical support for further research. [0003] In the past 30 year...

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Problems solved by technology

[0003] In the past 30 years, many methods have been developed to determine the GSM and 2-MIB in the water environment, but there are relatively few researches on the detection methods of earthy odor substances in freshwater fish at home and abroad, although the human nose can measure the GSM and 2-MIB in the water environment. Geosmin and 2-methylisoborneol can be detected within a certain range, but only semi-quantitative data can be provided, and the problem of sensory overload is prone to occur; the electronic nose with a special sensor can detect geosmin and 2-methylisoborneol, However, the sensitivity and selectivity in complex matrices such as fish cannot meet the requirements; the microwave distillation-solid phase microextraction method is not ideal due to the complexity of the device and low recovery rate

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