Method for determining earthy smell substances in freshwater fish

A method of determination, a technology of earthy smell, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unsatisfactory sensitivity and selectivity, unsatisfactory effect, complicated devices, etc., to avoid interference of impurities and easy simultaneous determination , the effect of high accuracy

Pending Publication Date: 2022-05-20
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

[0003] In the past 30 years, many methods have been developed to determine the GSM and 2-MIB in the water environment, but there are relatively few researches on the detection methods of earthy odor substances in freshwater fish at home and abroad, although the human nose can measure the GSM and 2-MIB in the water environment. Geosmin and 2-methylisoborneol can be detected within a certain range, but only sem

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  • Method for determining earthy smell substances in freshwater fish
  • Method for determining earthy smell substances in freshwater fish
  • Method for determining earthy smell substances in freshwater fish

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[0047] Example 1

[0048] The method of n-hexane extraction-solid phase extraction concentration-gas chromatography-mass spectrometry for the determination of earthy smell substances geosmin and 2-methylisobornol in freshwater fish, comprising the following steps:

[0049] Crucian carp, descaled, gutted, and skinned, take the muscle along the back, homogenize and mix well.

[0050] The first step, extraction: Weigh 5 g of minced crucian carp meat, add 15 mL of n-hexane, fully shake, ultrasonicate for 15 minutes, centrifuge at 4500 r / min for 8 minutes, take the supernatant into a 50-mL centrifuge tube; then add 10 mL of n-hexane to the fish, fully shake, ultrasonically Centrifuge at 4500r / min for 15min for 8min, take the supernatant and add it to a 50mL centrifuge tube.

[0051] The second step, purification: add 0.5g C18 powder, 0.5g graphitized carbon black, and 2g anhydrous magnesium sulfate to the centrifuge tube in the first step, shake for 7 minutes, and centrifuge at 25...

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Abstract

The invention discloses a method for determining earthy smell substances in freshwater fish. The method comprises the following steps: 1, extracting the earthy smell substances in freshwater fish paste twice by adopting normal hexane to obtain an extracting solution; step 2, adding C18 powder, graphitized carbon black and anhydrous magnesium sulfate into the extracting solution obtained in the step 1, fully oscillating, and centrifuging to achieve a purification effect; 3, purifying and concentrating the sample extracting solution by adopting a silica gel solid-phase extraction column to obtain an eluent; and 4, determining the content of the earthy substances in the eluent obtained in the step 3 by using a gas chromatography-mass spectrometry method. The method for determining the earthy smell substances in the freshwater fish is simple and easy to operate, higher in accuracy and better in stability.

Description

technical field [0001] The invention belongs to the technical field of food detection, and relates to a method for measuring geosmin (GSM) and 2-methylisoborneol (2-MIB), which are earthy smell substances in freshwater fish. Background technique [0002] my country's freshwater fish production is very large, accounting for 65% of the world's total, and plays a key role in the effective supply of aquatic products in the market. However, because freshwater fish generally has an unpleasant earthy smell, it seriously affects the taste and economic value of freshwater fish and its processed products, and restricts the healthy development of freshwater fish farming and processing industries. The substances that produce earthy smell are mainly Geosmin and 2-methylisoborneol. In view of this situation, it is urgent to establish an effective detection method for earthy odor substances in freshwater fish to provide technical support for further research. [0003] In the past 30 year...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/062Y02A40/81
Inventor 田良良胥亚夫孔聪蔡友琼方长玲沈晓盛
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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