Application of sodium tanshinone IIA sulfonate in preparation of anti-pancreatic fibrosis medicine
A technology for pancreatic fibrosis and tanshinone, applied in the field of medicine
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Embodiment 1
[0042] Example 1: Therapeutic effect of tanshinone IIA sodium sulfonate on cerulein-induced chronic pancreatitis and pancreatic fibrosis model mice (in vivo experiment)
[0043] The methods of grouping mice and making models are as follows:
[0044] C57BL / 6N male mice, 6-8 weeks old, body weight 20±2g, provided by Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd. The used sodium tanshinone IIA sulfonate was produced by Shanghai No. 1 Biochemical Pharmaceutical Co., Ltd., trade name: Nuoxinkang.
[0045] The temperature of the rearing room is 18-22°C, the relative humidity is 60%-75%, and the mice are fed with SPF grade chow.
[0046] Random grouping, 3 rats in each group: normal group, model group (Cae), low-dose group (Cae+STS 5mg / kg·d), middle-dose group (Cae+STS 10mg / kg·d), high-dose group (Cae+STS 20 mg / kg·d).
[0047] Specific administration method (Fig. 1A): In the normal group, 0.1 ml of PBS buffer was injected intraperitoneally, once a day. The mice in t...
Embodiment 2
[0061] Example 2: Tanshinone IIA sodium sulfonate can inhibit the activity of human pancreatic stellate cells (HPSCs) (in vitro experiment).
[0062] The specific method is as follows:
[0063] The HPSCs used in this study were purchased from Shanghai Hexu Biotechnology Co., Ltd. The used sodium tanshinone IIA sulfonate was produced by Shanghai No. 1 Biochemical Pharmaceutical Co., Ltd., trade name: Nuoxinkang. The CCK-8 reagent used was produced by DOJINDO, Japan. TGF-β1 cytokines were purchased from American R&D Company.
[0064] (2) Research steps:
[0065] ① The HPSCs were divided into the normal group, the TGF-β1 group and the tanshinone IIA sodium sulfonate-treated group. The normal group was only treated with DMEM medium containing 10% fetal bovine serum, and the TGF-β1 group and the drug-treated group were treated with transforming growth factor β1. (TGF-β1) at a dose of 10 ng / ml (diluted in the above medium) to stimulate HPSCs for 48 hours and then activate them. ...
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