Unlock instant, AI-driven research and patent intelligence for your innovation.

Columnar achilles tendon repair material and preparation method thereof

A repair material and columnar technology, applied in the fields of medical science, tissue regeneration, prosthesis, etc., can solve the problems of difficult to meet the mechanical guarantee of Achilles tendon, poor repair effect of Achilles tendon injury, easy recurrence, etc., to improve the effect of injury repair, The effect of good cell microenvironment and high biomechanical strength

Pending Publication Date: 2022-05-27
THE AFFILIATED HOSPITAL OF MEDICAL SCHOOL NINGBO UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Acute Achilles tendon rupture and Achilles tendon defect are generally treated with surgery, which may cause side injuries such as Achilles tendon adhesion and scar contracture.
The treatment of chronic Achilles tendinopathy includes physical therapy, drugs, cell therapy, etc., but it can only relieve the pain to a certain extent, and it is easy to relapse
[0003] After years of exploration, the development of tissue engineering technology has provided new ideas for solving Achilles tendon injuries. However, polylactic acid and polyglycolic acid are the most commonly used synthetic materials in tissue engineering tendon, which have poor biocompatibility and are prone to degradation Afterwards, it is acidic, and its components must be completely remodeled in the body before they can be used, and the effect of Achilles tendon injury repair is not good.
Moreover, the Achilles tendon is the continuation of the gastrocnemius and soleus muscles, and finally converges into a bundle of connective tissue with an oval structure. After the Achilles tendon defect, the tissue-engineered tendon with low biomechanical strength is difficult to meet the mechanical guarantee after the Achilles tendon repair.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Columnar achilles tendon repair material and preparation method thereof
  • Columnar achilles tendon repair material and preparation method thereof
  • Columnar achilles tendon repair material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Preparation of columnar Achilles tendon repair material

[0048] The preparation method of the columnar Achilles tendon repair material includes the following steps:

[0049] S1. Preparation of SIS scaffold

[0050] S11. Collect pig small intestine, cut it into small sections of about 5cm-10cm after cleaning, perform mechanical treatment, tear off the serosa and muscular layer of the small intestine, scrape off the mucosal layer and the mucosal muscular layer to obtain the SIS of the submucosa of the small intestine, and soak the SIS in In PBS buffer, shake and wash 3 times on a shaker, 5-30 min each time.

[0051]S12, soak the SIS obtained in step S11 in a mixture of methanol and chloroform, the volume ratio of methanol and chloroform in the mixture is 1:1-1:3, and shake on a shaking table for 4-12 hours to extract bound esters, Take out the SIS and soak it in PBS buffer, shake it on a shaker for 3 times, 5-30 min each time, and repeat the above steps once....

Embodiment 2

[0069] Example 2 Comparison before and after decellularization of small intestinal submucosa

[0070] 1. Frozen section production

[0071] Take the SIS before and after decellularization obtained in Example 1 for comparison, cut the SIS before and after decellularization to a size of about 1 cm × 1 cm, put it into an embedding box, add OCT embedding medium to completely cover the tissue, and put it in an ice cutter. To complete coagulation, take out the embedding block, place it on a small freezing table, put the sample head to clamp it, fine-tune the sample head, trim off the excess tissue on the surface to the target position and start slicing, set the thickness to 5-10μm, turn the handle to cut out the tissue, A coverslip is attached to the tissue.

[0072] 2. H&E staining

[0073] Take the SIS frozen glass slides before and after decellularization, stained with hematoxylin for 10 min, rinsed with tap water for 10 s, dried the slides, dripped with differentiation solutio...

Embodiment 3

[0078] Example 3 Physical Characterization of SIS Scaffolds

[0079] This example determines the Young's modulus and stress level of the SIS scaffold. Four kinds of SIS stents were made by crimping SIS sheets with 1-4 layers of SIS regularly stacked respectively, and the Achilles tendon and the four kinds of SIS stents were put on the test bench of the universal testing machine as samples for testing. The tensile rate was 0.1 mm / min. When the sample ruptured, the test was stopped and the value was recorded. Stress-strain curves of SIS scaffolds with different thicknesses were obtained. The peak stress is calculated from the linear portion of the stress-strain curve to obtain the maximum stress level. The result is as Figure 7 As shown, with the increase of the number of SIS layers, the maximum load gradually increased, and the maximum load of the SIS scaffold made by crimping the SIS sheet with 4 layers of SIS was similar to that of the normal Achilles tendon.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a columnar achilles tendon repair material and a preparation method thereof, the columnar achilles tendon repair material comprises a columnar SIS stent, and tendon stem cells are planted on the surface of the SIS stent. The columnar achilles tendon repair material is similar to a normal achilles tendon in form, has high biomechanical strength, can meet the mechanical guarantee after achilles tendon repair, and improves the injury repair effect; the repair material takes the columnar SIS as a biological scaffold and the tendon stem cells as seed cells, and has good biocompatibility and strong tendon differentiation capability.

Description

technical field [0001] The invention relates to the technical field of biological materials, in particular to a columnar Achilles tendon repair material and a preparation method thereof. Background technique [0002] Achilles tendon injury is one of the most common sports injuries in clinical practice, ranking third in human tendon rupture injuries. According to statistics, the incidence of Achilles tendon injury is 18 / 10,000, and the missed diagnosis rate of acute Achilles tendon rupture can be as high as 27% due to misdiagnosis by doctors and negligence of patients. If the missed diagnosis cannot be timely and properly treated for more than 4 weeks, it will develop into an old Achilles tendon rupture. Occurs in athletes and people with high work intensity, which has seriously affected people's work and life. Common Achilles tendon injuries include acute Achilles tendon rupture, Achilles tendon defect, and chronic Achilles tendinopathy. Acute Achilles tendon rupture and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/38A61L27/50A61L27/36
CPCA61L27/3804A61L27/386A61L27/50A61L27/3629A61L27/3662A61L27/3687A61L27/3691A61L2430/10A61L2430/40
Inventor 毛海蛟赵基源毛旭锋姚立炜张西前
Owner THE AFFILIATED HOSPITAL OF MEDICAL SCHOOL NINGBO UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com