Sample preparation compositions, devices, systems, and methods

Abstract

The present specification relates to compositions, devices, apparatuses, methods, kits, and systems for sample preparation (e.g., isolation, reduction, or removal of small molecules from biomolecules in a sample). The molecular weight range lt of exemplary small molecules that can be separated, reduced or removed; 2,000 Da, and may include, but not limited to, dyes, biotins, affinity tags, cross-linkers, reducing agents, labels, nanoparticles, radioligands, mass tags, unreacted molecules, and combinations, intermediates, and derivatives of the foregoing. Exemplary biomolecules present in a sample include, but are not limited to, proteins, glycoproteins, antibodies, peptides, nucleic acids, polysaccharides, carbohydrates, and lipids. The methods, compositions, kits, devices, apparatuses, and systems of the present disclosure can advantageously provide excellent separation of small molecule contaminants and additionally can reduce the time and cost associated with separating small molecules from larger biomolecules in a sample. Isolated biomolecules as shown herein are suitable for better downstream processing.

Description

technical field [0001] The present specification relates to compositions, devices, devices, methods, kits and systems for sample preparation (eg, separation of small molecules from larger molecules). In some embodiments, the compositions, devices, devices, systems, methods, and kits described herein can be used to separate, extract, purify, reduce, or remove larger molecules, such as, but not limited to, biomolecules in a sample Small molecule. Background technique [0002] Sample preparation techniques for isolating biomolecules aim to extract biomolecules from other sample components and from sample processing components to enable downstream analysis and processing of the biomolecules. For example, during sample preparation of biomolecules such as proteins or nucleic acids, it is often necessary to label the biomolecules with dyes, affinity tags, radioactive labels, mass tags, and the like. In other cases, chemical modification of biomolecules is required, such as reduct...

AI Technical Summary

Problems solved by technology

However, in addition to the disadvantages of dialysis and desalting resin-based methods listed above, protein recovery is poor with ion-exchange resins because proteins must be eluted from the ion-exchanger

Ion exchange methods for protein elution require multiple steps that when combined with the additional need for dialysis or desalting resins make the process time-consuming and tedious

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