Low-temperature-resistant saline-alkaline-tolerant phosphate solubilizing bacterium as well as culture method and application thereof

A low-temperature-resistant, salt-alkali-resistant technology, applied in the field of applied microorganisms, can solve the problems of ineffective phosphorus solubilization, inhibition of physiological activity, and microbial metabolic activities that are easily affected by temperature, so as to increase the content of soluble phosphate and reduce the amount of phosphate fertilizer Use, the effect of strong resistance to stress

Active Publication Date: 2022-06-17
浙江隆木环保科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In saline-alkali soil, the physiological activity of microorganisms will be inhibited due to saline-alkali stress, and most phosphate-solubilizing bacteria cannot effectively decompose phosphorus, and the metabolic activities of microorganisms are easily affected by temperature.

Method used

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  • Low-temperature-resistant saline-alkaline-tolerant phosphate solubilizing bacterium as well as culture method and application thereof
  • Low-temperature-resistant saline-alkaline-tolerant phosphate solubilizing bacterium as well as culture method and application thereof
  • Low-temperature-resistant saline-alkaline-tolerant phosphate solubilizing bacterium as well as culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] This example provides the application of a low-temperature-tolerant salt-alkali-tolerant phosphate-solubilizing bacterium (Acinetobacter calcoaceticus QY-3) to decompose inorganic phosphorus under low-temperature saline-alkali conditions.

[0026] The low-temperature-resistant salt-alkali-tolerant phosphorus-dissolving bacteria provided in this example grows on LB agar medium at 30°C for 24 hours, and its colony characteristics are: the colony is round, milky white in color, smooth in surface, sticky and easy to provoke, and the edge Complete; use scanning electron microscope to observe the morphology of the bacteria, such as figure 1 shown. The LB agar medium formula is as follows: 5 g of yeast extract, 10 g of NaCl, 10 g of tryptone, 16 g of agar powder, and 1000 ml of deionized water.

[0027] The present embodiment provides a basal medium for culturing the above-mentioned low-temperature-tolerant salt-alkali-tolerant phosphate-solubilizing bacteria, the basal mediu...

Embodiment 2

[0030] What this example provides is the screening and identification of Acinetobacter calcoaceticus QY-3 of the present invention:

[0031] (1) Screening of QY-3

[0032] Acinetobacter calcium acetate QY-3 provided in this example was isolated from the saline-alkali soil of Xiaokongtonggou, Qingyang City, Gansu Province, and was sampled in November 2020 and brought back to the laboratory for cryopreservation. Accurately weigh 20g of soil into a 250ml conical flask containing 160ml of sterile water, stir and mix well and let stand for 30min.

[0033]Take 20ml of supernatant in a 250ml conical flask containing 150ml of the inorganic phosphorus liquid basal medium described in Example 1, 15 ° C, 160rmp shaker culture for 3 days, the concentration of the cultured bacterial solution reaches 10 8 pcs / ml enriched bacterial solution.

[0034] Set the pH value 8, 10g / L NaCl in turn; pH value 8, 30g / L NaCl; pH value 8, 50g / L NaCl; pH value 9, 10g / L NaCl; pH value 9, 30g / L NaCl differ...

Embodiment 3

[0039] This example provides the determination of the ability of Acinetobacter calcoaceticus QY-3 to dissolve phosphorus under low-temperature alkaline conditions.

[0040] (1) Culture medium: an inorganic phosphorus liquid basal medium with a pH of 9 was used.

[0041] (2) Determination of the ability of strain QY-3 to dissolve phosphorus under low temperature and alkaline conditions

[0042] Activation and enrichment: After the strains are stored in cryopreservation tubes and activated, pick the strains and inoculate them into a 150ml conical flask containing 50ml of inorganic phosphorus liquid basal medium, cultivate at 15°C for 48h, and dilute to 10 cells. 8 pcs / ml.

[0043] According to the inoculum of 3%, it was inoculated into a 250ml Erlenmeyer flask containing 160ml of liquid basal medium. The strains were set up in three repeated shake flasks, and the sterile liquid medium was used as a control, and were cultured at 15°C and 160rmp. The content of soluble phosphoru...

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Abstract

The invention discloses a low-temperature-resistant saline-alkaline-tolerant phosphate solubilizing bacterium as well as a culture method and application thereof.The bacterium is separated from saline-alkaline soil in small Kongtong cave ditches in Qingyang City, Gansu Province, identified as Acinetobacter calcoaceticus and named as Acinetobacter calcoaceticus QY-3, the preservation unit of the bacterium is China Center for Type Culture Collection (CCTCC), the address is Wuhan University, Wuhan, China, the preservation date is 2021.1. 14, and the preservation number is CGMCC NO. The preservation number is CCTCC (China Center For Type Culture Collection) NO: M 2021067. The low-temperature-resistant saline-alkaline-tolerant phosphate solubilizing bacteria provided by the invention can tolerate saline-alkaline environments with pH values of 8 and 50 g / L NaCl, pH values of 9 and 30 g / L NaCl and pH values of 10 and 10 g / L NaCl to the maximum extent under a shaking table culture condition of 15 DEG C, and insoluble Ca3 (P04) 2 can be effectively converted into soluble phosphorus. The low-temperature-resistant saline-alkaline-tolerant phosphate solubilizing bacterium provided by the invention is high in stress resistance and has relatively high phosphate solubilizing capacity under low-temperature saline-alkaline conditions, so that the use of phosphate fertilizer in saline-alkaline land is reduced, and a solution is provided for saline-alkaline land treatment in China.

Description

【Technical field】 [0001] The invention relates to the technical field of applied microorganisms, in particular to a low-temperature-resistant salt-alkali-tolerant phosphorus-dissolving bacteria, a culture method and application thereof. [0002] 【technical background】 [0003] Saline-alkali land is an important reserve resource of cultivated land in my country, and its development and utilization have great potential in solving the shortage of cultivated land resources in my country. According to statistics, the total area of ​​saline-alkali land in my country is 99.13 million hectares. 2 , which has been reclaimed and utilized but has not reached 20%, and has the characteristics of large distribution area, wide range and large proportion of cultivated land. The salinization of the land deteriorates the physical and chemical properties of the soil and reduces the soil fertility, which in turn harms crop production. [0004] Phosphorus is one of the essential elements for pl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36C09K17/14C12R1/01
CPCC12N1/20C12N1/36C09K17/14
Inventor 翁为民刘永德陈春磊刘海张彦雄郝新伟沈锡辉毛婷婷
Owner 浙江隆木环保科技有限公司
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