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Culture medium for tissue culture of medicago ruthenica

A technology of tissue culture and culture medium, applied in the field of plant tissue culture, can solve the problems of low yield per unit area, achieve the effect of increasing the speed of culture, speeding up the speed of reproduction, and easy operation

Pending Publication Date: 2022-06-28
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, people's requirements for animal husbandry have been continuously improved, and the demand for alfalfa beans has gradually increased. However, in the prior art, the cultivation of alfalfa beans is all seed production, and its per unit yield level is low; therefore, the application of modern biological breeding Technology, establishment of tissue culture system to cultivate lentils has become the main research direction of development and utilization of lentils planting resources. Therefore, a medium for improving the reproductive speed and amount of lentils is needed

Method used

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  • Culture medium for tissue culture of medicago ruthenica
  • Culture medium for tissue culture of medicago ruthenica

Examples

Experimental program
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Effect test

Embodiment 1

[0023] The petioles of lentils with robust growth were selected as explants, and the explants were sterilized.

[0024] The sterilized explants were inoculated into the induction medium for callus culture, and the culture period was 12 days, the temperature was 20° C., and the humidity was 60%. The formula of the induction medium was: MS+0.18mg / L 6 -BA+0.48mg / L NAA+0.18mg / L 2,4-D+0.45g / L CH.

[0025] Preferably, the explant size is 0.27 mm.

[0026] Preferably, the disinfection method is as follows: 75% alcohol disinfection for 25s, mercuric solution disinfection for 2min, and finally using sterile water to wash for 4 times, each rinse for 25s.

[0027] The induction rate was measured, and the specific data are shown in Table 1.

Embodiment 2

[0029] The petioles of lentils with robust growth were selected as explants, and the explants were sterilized.

[0030] The sterilized explants were inoculated into the induction medium for callus culture, and the culture period was 17 days, the temperature was 28° C., and the humidity was 80%. The formula of the induction medium was: MS+0.22mg / L 6 -BA + 0.53 mg / L NAA + 0.22 mg / L 2,4-D + 0.55 g / L CH.

[0031] Preferably, the explant size is 0.30 mm.

[0032] Preferably, the disinfection method is as follows: 75% alcohol disinfection for 35s, mercuric solution disinfection for 4 minutes, and finally 6 times of sterile water cleaning, each rinse for 35s.

[0033] The induction rate was measured, and the specific data are shown in Table 1.

Embodiment 3

[0035] The petioles of lentils with robust growth were selected as explants, and the explants were sterilized.

[0036] The sterilized explants were inoculated into the induction medium for callus culture, and the culture period was 15 days, the temperature was 25° C., and the humidity was 70%. The formula of the induction medium was: MS+0.20mg / L 6 -BA + 0.50 mg / L NAA + 0.20 mg / L 2,4-D + 0.50 g / L CH.

[0037] Preferably, the explant size is 0.28 mm.

[0038] Preferably, the disinfection method is as follows: 75% alcohol disinfection for 30s, mercuric solution disinfection for 3 minutes, and finally using sterile water to wash for 5 times, each rinse for 30s.

[0039] The induction rate was measured, and the specific data are shown in Table 1.

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Abstract

A culture medium for inducing medicago ruthenica calluses comprises an induction culture medium and a subculture medium, the induction culture medium is prepared from MS, 0.18-0.22 mg / L of 6-BA, 0.48-0.53 mg / L of NAA, 0.18-0.22 mg / L of 2, 4-D and 0.45-0.55 g / L of CH, and the subculture medium is prepared from a culture medium, a subculture medium and a culture medium, the subculture medium is prepared from MS (Murashige and Skoog), 0.45 to 0.55 mg / L of 6-BA (Butyl Alcohol), 0.08 to 0.12 mg / L of NAA (Naphthalene Acetic Acid) and 0.45 to 0.55 g The invention provides the medium for inducing the medicago ruthenica calluses, the medicago ruthenica calluses can be rapidly obtained by adopting the medium, the inductivity and the proliferation rate of the medicago ruthenica calluses are improved, and the medium is a key link and a core technology for realizing tissue culture of the medicago ruthenica and construction of a genetic transformation system.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a medium for lentil tissue culture. Background technique [0002] Medicago ruthenica, belonging to the genus Leguminosae, is distributed in Northeast my country, Inner Mongolia, Ningxia, Gansu and other provinces. It is an asymmetrical "rhombus" and is a high-quality forage grass seed. It can be used as a mixed grass seed for artificial grassland or as a soil and water conservation plant. It is a leguminous forage with economical value. [0003] In recent years, people's requirements for animal husbandry have been continuously improved, and the demand for lentil beans has gradually increased. In the existing technology, the cultivation of lentil beans is seed production, and its unit yield is low; therefore, modern biological breeding is applied. Technology and establishment of tissue culture system to cultivate lentils have become the main research directions for th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002
Inventor 贾振宇马怀林张跃华刘亚玲屈璐璐闫士元白杨王晓龙杜聪郭秀芳
Owner INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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