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Controllable growth method of metal nanoparticles wrapped by DNA origami framework

A technology of metal nanoparticles and gold nanoparticles, which is applied in the biological field to achieve good assembly effect and enhanced effect of gold nanoparticles

Active Publication Date: 2022-06-28
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Currently, a method for the controlled growth of metal nanoparticles wrapped in DNA origami frameworks is lacking

Method used

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  • Controllable growth method of metal nanoparticles wrapped by DNA origami framework
  • Controllable growth method of metal nanoparticles wrapped by DNA origami framework
  • Controllable growth method of metal nanoparticles wrapped by DNA origami framework

Examples

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Effect test

Embodiment 1

[0024] This example is to control the growth of metal nanoparticles in DNA octahedral origami monomers.

[0025] A method for the controllable growth of metal nanoparticles wrapped in a DNA origami frame of the present invention includes the following steps: (1) Synthesizing the octahedral structure of DNA origami: M13mp18 DNA long chain and 112 kinds of DNA short chains in a ratio of 1:8 Mixed in 1×TAE, 12.5 mM MgAc 2 The solution was placed in a PCR machine for slow annealing at 95°C to 20°C for 22.5 h. Octahedral-shaped three-dimensional DNA origami nanostructures were prepared at a concentration of 10 nM;

[0026] (2) Modification of sulfhydryl DNA to the surface of gold nanoparticles: TCEP was added to the sulfhydryl-modified DNA single strand in a molar ratio of 200:9, and incubated at 0°C for 1 h, and then purified with a micro spin column; When using a micro spin column for purification, centrifuge in a centrifuge at 5000 rpm for 1.5 min; mix spherical gold nanoparti...

Embodiment 2

[0030] The difference between embodiment 2 and embodiment 1 is:

[0031] In this example, the controllable growth of metal nanoparticles in a two-dimensional plane formed by the connection of DNA octahedral origami.

[0032] A method for the controllable growth of metal nanoparticles wrapped in a DNA origami frame of the present invention includes the following steps: in step (1), synthesizing a DNA origami octahedral structure: combining the M13mp18 DNA long chain and 112 kinds of DNA short chains with 1 :10 in 1×TAE, 12.5 mM MgAc 2 The solution was placed in a PCR machine for slow annealing at 95°C to 20°C for 22.5 h. Octahedral-shaped three-dimensional DNA origami nanostructures were prepared at a concentration of 10 nM;

[0033]In step (2), modify the thiol DNA to the surface of gold nanoparticles: add TCEP to the thiol-modified DNA single-strand in a molar ratio of 200:8, conduct reduction incubation at 0 °C for 2 h, and then use microcentrifugation purify with a micro...

Embodiment 3

[0038] The difference between embodiment 3 and embodiment 2 is:

[0039] A method for the controllable growth of metal nanoparticles wrapped in a DNA origami frame of the present invention includes the following steps: in step (1), synthesizing a DNA origami octahedral structure: combining the M13mp18 DNA long chain and 112 kinds of DNA short chains with 1 : 12 in 1×TAE, 12.5 mM MgAc 2 in solution.

[0040] In step (2), modify the thiol DNA to the surface of gold nanoparticles: add TCEP to the thiol-modified DNA single-strand in a molar ratio of 200:8.5, conduct reduction incubation at 0°C for 1.5 h, and then use microcentrifugation Column purification; when using a micro spin column for purification, centrifuge in a centrifuge at 5000 rpm for 1 min; spherical gold nanoparticles with a particle size of 10 nm and purified thiol DNA in a molar ratio of 1:300 Proportional mixing reaction for 1.5 h, then adding PBbuffer to prepare a 10 mM PB buffer solution; then slowly adding 2...

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Abstract

The invention discloses a method for controllable growth of metal nanoparticles wrapped by a DNA origami framework. The method comprises the following steps: (1) synthesizing a DNA origami octahedral structure; (2) modifying sulfydryl DNA to the surfaces of the gold nanoparticles; (3) embedding the gold nanoparticles into the octahedron of the DNA origami: mixing the octahedron origami with the gold nanoparticles modified with the sulfydryl in a specific proportion, and putting the mixture into a PCR instrument for slow annealing to prepare metal nanoparticles wrapped by a DNA origami frame; connecting the two-dimensional planes; and (4) controllable growth of the gold nanoparticles: adding a gold enhancer Gold Enhancement into the octahedral-gold nanoparticle solution, so as to prepare the metal nanoparticles wrapped by the DNA origami framework after controllable growth. According to the invention, growth of the gold nanoparticles in the DNA origami octahedral framework is realized; quantitative control on particle size, form and the like of growth of the gold nanoparticles in the DNA framework is realized.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for the controllable growth of metal nanoparticles wrapped by a DNA origami frame. Background technique [0002] DNA origami is a technology that can build structures of various shapes based on the principle of complementary base pairing. In 2015, Tian Ye et al. constructed the regular octahedral origami structure for the first time (Article: Nature Nanotechnology, 2015, 10(7): 637-44.). In 2020, metal nanoparticles were carried on the interior cavity of octahedral origami (article: Angew.Chem. Int. Ed. 2020, 59, 6389.), and the origami octahedron was used as a structural unit through base pairing. The assembly and construction of two-dimensional and three-dimensional structures were carried out, and the construction of micro- and nano-scale systems was realized. This origami system has many advantages, such as high order and structural controllability, which make it have ...

Claims

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Application Information

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IPC IPC(8): B22F1/102B22F1/054B22F1/07B22F1/145B22F9/16C12N15/10
CPCB22F9/16C12N15/10C12Q2531/113C12Q2527/125C12Q2523/32
Inventor 田野曹文虹解晓琳季旻
Owner NANJING UNIV