System for large-scale adherent cell culture
An adherent cell, large-scale technology, used in tissue cell/virus culture devices, specific-purpose bioreactors/fermenters, biomass post-processing, etc. Insufficient supply and other problems to achieve the effect of accelerating exchange, accelerating flow, and increasing gas exchange rate
Active Publication Date: 2022-07-05
山东壹瑞特生物科技有限公司
View PDF4 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0006] Cultivating bioactive hepatocytes is a prerequisite for artificial liver support therapy. Cell reactors are the main device for culturing hepatocytes. The current cell reactors have the following disadvantages: (1) The supply of nutrients, oxygen and other substances required for cell growth Insufficient; (2) The cultured cells are not easy to take out
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0038] Below in conjunction with the accompanying drawings, various specific embodiments of the present invention will be described in detail. The terms “upper, lower, left, right, front, rear” mentioned in the embodiments are for the convenience of describing one of the The relationship of an element or feature to another element or feature(s). These spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. The device may be turned in different orientations (rotated 90 degrees or otherwise) and the spatially relative terms used herein are to be interpreted in the same way.
[0039] figure 1 It is a front view structure schematic diagram of the present invention, figure 2 It is a schematic diagram of the overall structure of the present invention. This embodiment discloses a system for culturing adherent cells on a large scale, which includes a cell reactor 1 and a conn...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a large-scale adherent cell culture system which comprises a cell reactor and a connecting pipe, the cell reactor comprises an input port and an output port, the input port and the output port are both communicated with the connecting pipe, a circulating pump and an aerator are arranged on a pipeline of the connecting pipe, the circulating pump is arranged at one end of the input port, and the aerator is arranged at the other end of the input port. And the aerator is arranged at one end of the output port. The circulating pump is beneficial to accelerated flow of a culture solution in the bioreactor, components in the culture solution are kept uniform, and cell gas exchange and nutrient supply are accelerated; the use of the oxygen increasing device can greatly increase the gas exchange rate between the gas and the culture solution, and meanwhile, the generation of a large number of bubbles in a liquid loop caused by direct ventilation is avoided.
Description
technical field [0001] The invention belongs to the technical field of cell culture, in particular to a system for culturing adherent cells on a large scale. Background technique [0002] As one of the important organs of the human body, the liver is also called "processing factory" because of its functions of synthesis, detoxification, metabolism, secretion, biotransformation and immune defense. The liver is composed of liver cells and has a rich vascular network. It is reddish-brown, soft and brittle, and is easily ruptured by violent blows, causing fatal hemorrhage. Liver cells are so small that they are invisible to the naked eye and must be seen through a microscope. The human liver has about 2.5 billion hepatocytes, and 5,000 hepatocytes form a hepatic lobule, so the total number of hepatic lobules in the human liver is about 500,000. [0003] When serious damage is caused by various factors (such as viruses, alcohol, drugs, etc.), it will cause a large number of nec...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/04C12M1/34C12M1/04
CPCC12M25/10C12M29/00C12M29/18C12M29/26C12M41/00C12M41/32C12M41/12C12M41/26
Inventor 阮海斌D·贺J·布拉泽顿齐建国户家航张丛丛董雨蝶
Owner 山东壹瑞特生物科技有限公司