Flow cytometer based on confocal light path design

Abstract

The invention discloses a flow cytometer based on confocal light path design, and belongs to the technical field of life analysis scientific detection equipment. The flow cytometer comprises a liquid flow system, an optical system and a detection system, the liquid flow system comprises an injection pipeline and a sheath fluid pipeline which are respectively communicated with the quartz flow cell, the injection pipeline injects a cell sample into a central sheath fluid channel of the quartz flow cell, and the cell sample is wrapped and extruded by sheath fluid injected into the central sheath fluid channel of the quartz flow cell by the sheath fluid pipeline to form single cell flow; the optical system comprises an excitation light path and a fluorescence collection light path, a laser beam of the excitation light path is focused on a central sheath flow channel of the quartz flow cell and is used for exciting a cell sample in the central sheath flow channel of the quartz flow cell to generate fluorescence, and the fluorescence is collected through the fluorescence collection light path; the detection system comprises a photoelectric detector, a data acquisition card and an upper computer which are connected in sequence. The flow cytometer is compact in structure, simple and efficient in light path focusing and convenient to use.

Description

technical field [0001] The invention relates to the technical field of life analysis scientific detection equipment, in particular to a flow cytometer based on confocal optical path design. Background technique [0002] Cells are the most basic unit that constitutes life and completes life activities, and there is general heterogeneity between cells. Therefore, characterizing life activities at the single-cell level has become a life science research such as proteomics and metalloomics. The technical means necessary in the medical field such as the diagnosis and treatment of diseases and diseases. Flow cytometry is currently the most widely used high-throughput single-cell multi-parameter analysis technology. Flow cytometer can obtain quantitative information of intracellular biomolecules such as fluorescently labeled proteins, and information such as cell structure and morphology through the optical and fluorescent properties of single cells in sheath flow when they pass t...

AI Technical Summary

Problems solved by technology

[0006] In order to solve the technical problems of existing flow cytometers such as low sensitivity, complex optical path adjustment, cumbersome use process and test parameter setting, and high price, the present invention provides a flow cytometer based on confocal optical path design. Analyzer

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