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Automatic rapid detection instrument and detection method for Raman drug resistance of microorganisms

A Raman drug resistance and detection instrument technology, applied in the field of microbial detection, can solve the problems of inability to quickly locate the sample position, inability to detect plaques, and slow detection speed, so as to reduce the difficulty of focusing, reduce detection costs, and reduce detection. effect of time

Pending Publication Date: 2022-05-13
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing detection instruments using Raman technology can only detect single-cell samples, which are suitable for slow-growing and difficult-to-cultivate microorganisms, and cannot quickly locate the sample position. The detection speed is slow and the efficiency is low, and it cannot detect plaque

Method used

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  • Automatic rapid detection instrument and detection method for Raman drug resistance of microorganisms
  • Automatic rapid detection instrument and detection method for Raman drug resistance of microorganisms
  • Automatic rapid detection instrument and detection method for Raman drug resistance of microorganisms

Examples

Experimental program
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Effect test

Embodiment 1

[0056] Such as figure 1 As shown, the automated rapid detection instrument for microbial Raman drug resistance provided in this embodiment includes a motorized displacement platform, an imaging module, an excitation light module, a microfocusing module, a Raman main optical path and a transmission module, a coaxial lighting module, and Automatic acquisition control module;

[0057] The electric displacement platform is used to place the sample chip, cooperate with the imaging module to realize the automatic focus of the sample, and quickly determine the position of the plaque;

[0058] The imaging module is used to capture the panoramic information of the sample chip, obtain the position information of each bacterial plaque, and form the position correction information obtained by comparing each bacterial plaque with the preset bacterial plaque; at the same time, it is used to capture the microscopic image of the bacterial plaque, and cooperates with The electric translation ...

Embodiment 2

[0096] Such as Figure 4 As shown, the detection method of the automated microbial Raman drug resistance rapid detection instrument provided in Example 1 comprises the following steps:

[0097] S01, the imaging module takes an image of the entire sample chip, and obtains position information of each plaque to be tested;

[0098] Specifically, the sample is produced:

[0099] Mix the obtained monoclonal samples with the medium, establish a control group, add different concentrations and types of antibiotics and water; in addition, establish an experimental group, add different concentrations and types of antibiotics and the same water as the control group in the medium. The same volume of heavy water D 2 O. The control and experimental groups were incubated for the same time. The bacterial solution of the control group and the experimental group was pretreated and the detection chip was made, the detection chip was placed on the electric displacement platform, and the panor...

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Abstract

The invention relates to an automatic microorganism Raman drug resistance rapid detection instrument and method, and the device comprises an electric displacement platform, an imaging module, an excitation light module, a microscopic focusing module, a Raman main light path and transmission module, a coaxial illumination module, and an automatic collection control module. The electric displacement platform is used for placing a sample chip; the excitation light module is used for emitting laser; the microscopic focusing module is used for automatically focusing the laser to the to-be-detected bacterial plaque at the optimal acquisition position, so that the to-be-detected bacterial plaque generates a Raman signal; the coaxial illumination module is used for providing coaxial illumination light for the microscopic focusing module; the Raman main light path and transmission module is used for acquiring a Raman spectrum of the bacterial plaque to be detected; and the automatic acquisition control module is used for controlling the exciting light module, the Raman main light path and transmission module, the microscopic focusing module, the coaxial illumination module, the imaging module and the electric displacement platform, and processing the collected Raman spectrum of the to-be-detected bacterial plaque, so that automatic detection and rapid drug resistance identification of the to-be-detected bacterial plaque are realized.

Description

technical field [0001] The invention relates to the technical field of microorganism detection, in particular to an automatic rapid detection instrument and detection method for Raman drug resistance of microorganisms. Background technique [0002] The traditional clinical detection of drug resistance of pathogenic bacteria is mainly through the culture method, which has the disadvantages of long detection time, high professional requirements for experimental operators, and prone to false positives. To curb the spread of drug resistance, it is not only necessary to develop new antibiotics, but also to develop rapid detection instruments and methods for drug resistance to improve the pertinence and effectiveness of the use of existing antibiotics, thereby delaying and curbing the spread of drug resistance. [0003] At present, one of the most promising directions is the "single cell" drug resistance detection technology, which skips cell culture proliferation and directly cha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/01G01N21/65G01B11/00
CPCG01N21/01G01N21/65G01B11/00G01N2201/06113
Inventor 李远东任立辉刘晓颖籍月彤马波徐健
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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