Quantitative detection method for creatine and creatinine in whole blood

Abstract

The invention relates to a quantitative detection method for creatine and creatinine in whole blood, which comprises the following steps: detecting by using a creatine-creatinine combined detection test strip to obtain hemoglobin detection concentration, creatine detection concentration and creatinine detection concentration of a whole blood sample, and determining a correction coefficient through the hemoglobin detection concentration; the creatine detection concentration and the creatinine detection concentration are corrected, the creatine concentration of the whole blood sample is equal to the creatine detection concentration * the correction coefficient, the creatinine concentration of the whole blood sample is equal to the creatinine detection concentration * the correction coefficient, and the determination method of the correction coefficient comprises the following steps: setting a reference value of the hemoglobin detection concentration; the correction coefficient = (hemoglobin detection concentration / reference value) * correction factor alpha, and if the hemoglobin detection concentration is greater than or equal to the reference value, the correction factor alpha is less than 1; if the hemoglobin detection concentration is smaller than the reference value, the correction factor alpha is larger than 1. According to the method, the influence of endogenous creatine and hemoglobin on the detection of the creatinine concentration of the whole blood sample can be eliminated, and a more accurate detection result can be obtained in a short time.

Description

technical field [0001] The invention relates to a quantitative detection method for creatine and creatinine in whole blood. Background technique [0002] Creatinine is a product of muscle metabolism in the human body and is mainly excreted by glomerular filtration. Creatinine is a low molecular weight nitrogenous compound with a molecular weight of 116D. Blood creatinine comes from both exogenous and endogenous sources. Exogenous creatinine is the product of meat metabolism in the body; endogenous creatinine is the product of muscle tissue metabolism in the body. Serum creatinine is mainly excreted in the urine by the kidneys. Plasma creatinine comes from muscle tissue, and its concentration is proportional to muscle mass. Therefore, in acromegaly and gigantism, the plasma creatinine concentration increases; on the contrary, in muscular dystrophy diseases, the plasma creatinine concentration decreases. Before and after dialysis treatment, plasma creatinine measurement can...

AI Technical Summary

Problems solved by technology

[0003] The determination of creatinine concentration is easily interfered by various endogenous and exogenous factors

Creatine is an intermediate product of creatinine detection. Endogenous creatine will negatively interfere with the detection of low and high concentrations of creatinine at a certain concentration, affecting the accuracy of creatinine detection.

When the test sample is whole blood, hemoglobin will have an impact on the test results, which is unfavorable

The detection of plasma samples can avoid the interference of hemoglobin on the test results, but professional technicians are required to preprocess the whole blood samples, which is complicated and time-consuming

Although the existing creatinine detection test paper using whole blood samples adds a filter membrane that can remove red blood cells before the samples reach the reaction layer, the plasma content in different whole blood samples is different, so that the amount of samples that finally reach the reaction layer is different, resulting in test results large deviation

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