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Hu sheep SLC27A6 gene SNP detection method and application of Hu sheep SLC27A6 gene SNP detection method in meat quality character early screening

A gene and genome technology, applied in the field of single nucleotide polymorphism sites of Hu sheep SLC27A6 gene, to achieve the effect of accelerating breeding

Pending Publication Date: 2022-07-08
甘肃润牧生物工程有限责任公司
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Problems solved by technology

[0005] Although SLC27A6 plays an important role in fatty acid transport and lipid metabolism, there is no report about the SNP in the sheep SLC27A6 gene affecting the fatty acid phenotype, and it is lacking that it can be used to breed the fatty acid phenotype of sheep and comprehensively improve meat quality Molecular markers

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  • Hu sheep SLC27A6 gene SNP detection method and application of Hu sheep SLC27A6 gene SNP detection method in meat quality character early screening
  • Hu sheep SLC27A6 gene SNP detection method and application of Hu sheep SLC27A6 gene SNP detection method in meat quality character early screening
  • Hu sheep SLC27A6 gene SNP detection method and application of Hu sheep SLC27A6 gene SNP detection method in meat quality character early screening

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Embodiment Construction

[0026] The present invention will be described in further detail below with reference to the accompanying drawings and embodiments. The embodiments are only used to explain the present invention, but not to limit the protection scope of the present invention.

[0027] 1. The SNP locus of Hu sheep SLC27A6 gene was obtained by DNA pool sequencing

[0028] S1 sample selection

[0029] From August 2018 to August 2021, a total of 1037 (5 batches) testicles of 6-month-old healthy Hu sheep were collected from Minqin Defu Agricultural Technology Co., Ltd. (Minqin County, Wuwei, Gansu Province) under the same feeding conditions. tissue and longissimus dorsi muscle tissue, and determine the fatty acid composition and content of the longissimus dorsi muscle tissue.

[0030] S2 constructs DNA pool

[0031] a) Use the phenol-chloroform method to extract the genomic DNA of the testis tissue of 1037 Hu sheep individuals, and the specific steps are as follows:

[0032] 1) get 10mg ground ...

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Abstract

The invention discloses a Hu sheep SLC27A6 gene SNP (Single Nucleotide Polymorphism) detection method and application of the Hu sheep SLC27A6 gene SNP detection method in early screening of meat quality characters. According to the invention, Hu sheep tissue DNA is extracted, an iMLDR technology is used for typing, allele frequency and genotype frequency are calculated, and Hu sheep back longissimus fatty acid percentage and SLC27A6 gene mutation site g.504Ggt; and C, carrying out correlation analysis to reveal the polymorphism of the site and the existing molecular marker significantly related to the fatty acid phenotype, and providing a basis for selecting and breeding a new sheep strain with excellent meat quality through marker-assisted selection.

Description

technical field [0001] The invention belongs to the field of molecular genetic testing and breeding, and in particular relates to a Hu sheep SLC27A6 gene single nucleotide polymorphism (Single nucleotide polymorphism, SNP) site detected by DNA mixed pool sequencing, combined with a fluorescent multiple enzyme ligation reaction ( Improved multiplex ligation detection reaction, iMLDR) technology was used to genotype the locus and use the genotype results to conduct correlation analysis with fatty acid percentage. Background technique [0002] SNPs are widely present in the genome, with approximately one SNP occurring every 300 bp of sequence length. SNPs can be divided into coding region SNPs and non-coding region SNPs according to the location of mutation. The former mainly affects the phenotype by affecting the structure of the protein. At present, screening SNPs related to important traits plays an important role in genetic improvement. iMLDR technology is a new high-thro...

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2600/124C12Q2531/113Y02P60/87
Inventor 乐祥鹏孔园园张兆才李建栋李宝胜叶炜董彦琴赵锦龙赵海庆
Owner 甘肃润牧生物工程有限责任公司
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