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Application of CO2 in increasing activity of endogenous and exogenous digestive enzymes in intestinal tracts of termites

A technology for digestive enzymes and termites, applied in the field of insects, can solve problems such as affecting the degradation of lignocellulose and increasing the activity of endogenous and exogenous digestive enzymes of termites

Active Publication Date: 2022-07-15
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]Currently, it is unclear how CO2 concentration affects the activity of digestive enzymes in Coptotermes formosanus, thereby affecting the process of degrading lignocellulose
There is also no report that CO2 can increase the activity of endogenous and exogenous digestive enzymes in termite gut

Method used

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  • Application of CO2 in increasing activity of endogenous and exogenous digestive enzymes in intestinal tracts of termites
  • Application of CO2 in increasing activity of endogenous and exogenous digestive enzymes in intestinal tracts of termites
  • Application of CO2 in increasing activity of endogenous and exogenous digestive enzymes in intestinal tracts of termites

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Experimental program
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Effect test

Embodiment 1

[0018] 1. Experimental method

[0019] 1. Food handling

[0020] Sugarcane straw, straw straw and pine sawdust were ground into powder, sterilized in an autoclave at 121°C for 30 minutes, dried, and pressed into a solid powder for feeding termites.

[0021] 2. Feeding treatment of test termites

[0022] The test workers were fed after starvation for 24 hours, and 50 sterile fine sand, corresponding food powder and healthy workers were placed in a petri dish with a diameter of 35 mm. The petri dishes were placed in a carbon dioxide artificial climate incubator with a temperature of 30 ± 1 °C and a relative humidity of 70 ± 5%, and the above three kinds of food were fed to L. taiwanae for 10 days. The air treatment group did not set CO. 2 Concentration (CO 2 concentration is about 0.04%), CO 2 The concentrations of the treatment groups were set at 1%, 5% and 10%, respectively.

[0023] 3. Determination of enzyme activity

[0024] Select 20 worker ants, in SAB solution (ace...

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Abstract

The invention discloses an application of CO2 in increasing the activity of endogenous and exogenous digestive enzymes in intestinal tracts of termites. The invention finds that CO2 enhances endogenous and exogenous EG and EX enzyme activities of Coptotermes formosanus and promotes the decomposition of cellulose and hemicellulose of Coptotermes formosanus.

Description

technical field [0001] The present invention relates to the field of insects, in particular to CO 2 Use in increasing the activity of endogenous and exogenous digestive enzymes in the gut of termites. Background technique [0002] Lignocellulose is the most abundant renewable resource in the world (Zaldivar J, Nielsen J, Olsson L. Fuel ethanol production from lignocellulose: a challenge for metabolic engineering and process integration[J].Appl Microbiol Biotechnol,2001,56(1-2) : 17-34.), mainly composed of cellulose, hemicellulose and lignin (Madadi M, Abbas A, TuY.Pretreatment of lignocellullusic biomass based on improving enzymatichydrolysis[J].International Journal of Applied Sciences and Biotechnology, 2017, 5(1):1-11.). Enzymatic hydrolysis of lignocellulose is an indispensable way in the process of resource utilization of straw, and the degradation of lignocellulose requires the coordination of multiple enzymes. In the process of cellulose degradation to glucose, en...

Claims

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Application Information

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IPC IPC(8): A01K67/033C12Q1/34
CPCA01K67/033C12Q1/34G01N2333/924G01N2333/942Y02E50/10
Inventor 吴文静李志强张世军刘炳荣周虹冯淑怡
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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